Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

PRKX S4166-p
 Putative in vivo kinases: 

An enzyme-substrate reaction that occurs within living cells; includes cultured cells, ex vivo samples, and intact organisms. In the case of kinases, the large number of protein kinases in intact cells makes exact identification of the responsible kinase challenging.

PKACA S4252-p
PRKX S4166-p
Src Y4237-p
Putative upstream phosphatases:
PPP1CA S4169-p
PTPRG Y4237-p
Treatment:
BI2536 S843-p , T856-p , T858-p