Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

ERK2 S247-p
Treatment:
insulin S243-p , S383-p
LY294002 S383-p
MK-2206 S383-p
Trametinib S247-p