Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

Akt1 T70-p
 Putative in vivo kinases: 

An enzyme-substrate reaction that occurs within living cells; includes cultured cells, ex vivo samples, and intact organisms. In the case of kinases, the large number of protein kinases in intact cells makes exact identification of the responsible kinase challenging.

ERK2 T221-p
Regulatory protein:
leptin T221-p
insulin S209-p
long-term_potentiation T221-p
okadaic_acid T220-p , T221-p
SB202190 S224-p
starvation T70-p