Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

PDK1 S381-p
 Putative in vivo kinases: 

An enzyme-substrate reaction that occurs within living cells; includes cultured cells, ex vivo samples, and intact organisms. In the case of kinases, the large number of protein kinases in intact cells makes exact identification of the responsible kinase challenging.

PDK1 S381-p
Treatment:
EGF S381-p
serum S381-p , S398-p
U0126 S381-p , S398-p