Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

Lck Y340-p
Putative upstream phosphatases:
SHP-1 Y340-p
SHP-2 Y340-p
Treatment:
vanadate Y340-p