Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

JAK2 Y204-p , Y221-p
 Putative in vivo kinases: 

An enzyme-substrate reaction that occurs within living cells; includes cultured cells, ex vivo samples, and intact organisms. In the case of kinases, the large number of protein kinases in intact cells makes exact identification of the responsible kinase challenging.

Lck Y204-p , Y221-p
Treatment:
cycloheximide Y204-p , Y221-p
EGF Y204-p , Y221-p
IL-10 Y204-p
imatinib Y204-p
LPS Y204-p , Y221-p
PDGF Y221-p
vanadate Y204-p , Y221-p