Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

Chk1 T140-p , S141-p
 Putative in vivo kinases: 

An enzyme-substrate reaction that occurs within living cells; includes cultured cells, ex vivo samples, and intact organisms. In the case of kinases, the large number of protein kinases in intact cells makes exact identification of the responsible kinase challenging.

Abl Y76-p
Akt1 T140-p , S141-p
Chk1 T140-p , S141-p
NuaK1 S78-p , S141-p