Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

ERK1 T321-p , S346-p , S376-p
ERK2 T321-p , S346-p , S376-p
Treatment:
serum T321-p , S346-p , S376-p
U0126 T321-p , S346-p , S376-p