Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

PIK3CA Y515-p
Treatment:
IL-33 Y515-p
insulin Y488-p
vanadate Y488-p , Y515-p
wortmannin Y515-p