Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

Akt1 S291-p
CDK1 S291-p
 Putative in vivo kinases: 

An enzyme-substrate reaction that occurs within living cells; includes cultured cells, ex vivo samples, and intact organisms. In the case of kinases, the large number of protein kinases in intact cells makes exact identification of the responsible kinase challenging.

Akt1 S291-p
Treatment:
Akt_inhibitor_VIII S291-p
calyculin_A S291-p
Cdk2_inhibitor_II S291-p
nocodazole S291-p
okadaic_acid S291-p
RO-3306 S291-p