Kinase, in vitro: 

An enzyme-substrate reaction that occurs in non-living experimental conditions such as a test tube. For example, a purified enzyme is reacted with a substrate protein or mixture of proteins or peptides.

MEKK1 S271-p , T275-p
Regulatory protein:
Akt1 S271-p
Treatment:
anisomycin S271-p
ischemia S35-p , S271-p
MG132 K373-ub
nocodazole S35-p