IkB-alpha (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

K21-ub

EGPRDGLkkERLLDD

Downstream

Effects on Modified Protein

activity, induced
intracellular localization
protein degradation
ubiquitination

Effects on Biological Processes:

transcription, induced

IkB-alpha (human)	EGPRDGLkkERLLDD	K21-ub
IkB-alpha (mouse)	EGPRDGLkkERLVDD	K21-ub
IkB-alpha (rat)	EGPRDGLKKERLVDD	K21

K21-sm

EGPRDGLkkERLLDD

Upstream

Downstream

Effects on Modified Protein

activity, induced
protein stabilization
ubiquitination

Effects on Biological Processes:

transcription, induced
transcription, inhibited

Regulatory protein:

RWDD3 (human)

Treatment

hypoxia
siRNA

IkB-alpha (human)	EGPRDGLkkERLLDD	K21-sm
IkB-alpha (mouse)	EGPRDGLkkERLVDD	K21-sm
IkB-alpha (rat)	EGPRDGLKKERLVDD	K21

K22-ub

GPRDGLkkERLLDDR

Downstream

Effects on Modified Protein

activity, induced
intracellular localization
protein degradation
ubiquitination

Effects on Biological Processes:

transcription, induced

IkB-alpha (human)	GPRDGLkkERLLDDR	K22-ub
IkB-alpha (mouse)	GPRDGLkkERLVDDR	K22-ub
IkB-alpha (rat)	GPRDGLKKERLVDDR	K22

K22-sm

GPRDGLkkERLLDDR

Upstream

Downstream

Effects on Modified Protein

protein stabilization

Effects on Biological Processes:

transcription, inhibited

Regulatory protein:

RWDD3 (human)

IkB-alpha (human)	GPRDGLkkERLLDDR	K22-sm
IkB-alpha (mouse)	GPRDGLkKERLVDDR	K22
IkB-alpha (rat)	GPRDGLKKERLVDDR	K22

S32-p

LLDDRHDsGLDsMkD

Upstream

Downstream

173

IkB-alpha (human)
S32-p

IkB-alpha (mouse)
S32-p

IkB-alpha (rat)
S32-p

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
phosphorylation
protein degradation
protein stabilization
sumoylation
ubiquitination

Effects on Biological Processes:

apoptosis, altered
apoptosis, induced
apoptosis, inhibited
cell growth, induced
signaling pathway regulation
transcription, altered
transcription, induced

Kinase, in vitro:

AurA (human)
AurB (human)
AurC (human)
CK2A1 (human)
ERK7 (human)
IKKA (human)
IKKB (human)
Nik (human)
p90RSK (human)
RSK2 (human)
SGK1 (human)

Putative in vivo kinases:

AurC (human)
ERK7 (human)
IKKA (human)
IKKB (human)
IKKE (human)
RSK2 (human)

Regulatory protein:

AGK (human)
AlphaK3 (human)
Bcl-10 (human)
CAMK2D (human)
CARD11 (human)
CK2A1 (human)
CYBB (human)
DDX3 (human)
DHX58 (human)
EGFR (human)
EGLN1 (human)
EGLN2 (human)
EGLN3 (human)
FABP5 (human)
FGFR1 (human)
HIF1A (human)
HOOK3 (human)
IkB-alpha (human)
IKKB (human)
IKKG (human)
LMP1 (herpesvirus)
MARCH3 (human)
MAVS (human)
MDA5 (human)
p53 (human)
PKR (mouse)
PLCE1 (human)
PLK1 (human)
PRKD2 (human)
PTCH1 (human)
RBCK1 (mouse)
RIGI (human)
RNF31 (human)
SGK1 (human)
SHIP (human)
TAK1 (human)
TIFA (human)
TR-beta1 (human)
TRAF2 (human)
TRAF3 (human)
TRAF6 (human)
TRIM37 (human)
UBE2D1 (human)
UBE2N (human)

Treatment

(5Z)-7-oxozeaenol
15d-PGJ2
4miRs
actinomycin_D
antibody
aspirin
bacterial infection
Bay_11-7082
betulinic_acid
bisindolylmaleimide
bortezomib
bradykinin
BSO
cAMP_analog
carrageenan
ciclosporin
cisplatin
cycloheximide
D4
deferoxamine
dexamethasone
dimethylfumarate
DPI
EGCG
EGF
Erastin
estradiol
etoposide
FeTPPS
FIR
GBS
GM-CSF
gomisin_A
gomisin_N
H2O2
heregulin
hypoxia
hypoxia/reoxygenation
IGF-1
IL-1a
IL-1b
IL-4
ionizing_radiation
ionomycin
KN-93
KP-392
LLnL
LPA
LPS
MG115
MG132
MLN4924
monomethyl fumarate
NAC
NGF
nocodazole
okadaic_acid
ONOO(-)
osmotic_stress
PAR1-activating_peptide
PAR2-activating_peptide
parthenolide
PD98059
PEITC
peptide
phorbol_ester
piceatannol
poly(I-C)
pomegranate_wine
RSL3
SB203580
SBHA
SDR
seliciclib
siRNA
staurosporine
sulforaphane
taxol
tempol
testosterone
thapsigargin
thrombin
TNF
TRAIL
U0126
UV
vanadate
virus infection
Z-VAD-FMK

IkB-alpha (human)	LLDDRHDsGLDsMkD	S32-p
IkB-alpha (mouse)	LVDDRHDsGLDsMKD	S32-p
IkB-alpha (rat)	LVDDRHDsGLDsMKD	S32-p

S36-p

RHDsGLDsMkDEEyE

Upstream

Downstream

104

IkB-alpha (human)
S36-p

IkB-alpha (mouse)
S36-p

IkB-alpha (rat)
S36-p

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
phosphorylation
protein degradation
protein stabilization
sumoylation
ubiquitination

Effects on Biological Processes:

apoptosis, altered
apoptosis, induced
apoptosis, inhibited
carcinogenesis, induced
cell growth, induced
cell motility, induced
transcription, altered
transcription, induced

Kinase, in vitro:

CK2A1 (human)
ERK7 (human)
IKKA (human)
IKKB (human)
Nik (human)

Putative in vivo kinases:

ERK7 (human)
IKKA (human)
IKKB (human)
IKKE (human)

Regulatory protein:

abLIM (human)
CAMK2D (human)
CARD11 (human)
CK2A1 (human)
EGFR (human)
EGLN1 (human)
EGLN2 (human)
EGLN3 (human)
HIF1A (human)
IKKG (human)
LMP1 (herpesvirus)
PLK1 (human)
PRKD2 (human)
RBCK1 (mouse)
RNF31 (human)
SHIP (human)
TAK1 (human)
TR-beta1 (human)
TRIM37 (human)
UBE2D1 (human)
UBE2N (human)

Treatment

15d-PGJ2
4miRs
actinomycin_D
antibody
bacterial infection
Bay_11-7082
betulinic_acid
bisindolylmaleimide
BSO
cisplatin
cycloheximide
deferoxamine
dexamethasone
dimethylfumarate
EGCG
EGF
Erastin
etoposide
FeTPPS
gomisin_A
gomisin_N
H2O2
hypoxia
hypoxia/reoxygenation
IL-1a
IL-1b
ionizing_radiation
ionomycin
KN-93
LLnL
LPA
LPS
MG132
monomethyl fumarate
NAC
nocodazole
okadaic_acid
ONOO(-)
PAR1-activating_peptide
PAR2-activating_peptide
parthenolide
peptide
phorbol_ester
pomegranate_wine
RSL3
SBHA
seliciclib
siRNA
taxol
thapsigargin
thrombin
TNF
U0126
UV
vanadate
virus infection

IkB-alpha (human)	RHDsGLDsMkDEEyE	S36-p
IkB-alpha (mouse)	RHDsGLDsMKDEEYE	S36-p
IkB-alpha (rat)	RHDsGLDsMKDEDyE	S36-p

K38-ub

DsGLDsMkDEEyEQM

IkB-alpha (human)	DsGLDsMkDEEyEQM	K38-ub
IkB-alpha (mouse)	DsGLDsMKDEEYEQM	K38
IkB-alpha (rat)	DsGLDsMKDEDyEQM	K38

Y42-p

DsMkDEEyEQMVkEL

Upstream

Downstream

Effects on Modified Protein

activity, induced
molecular association, regulation
protein degradation

Effects on Biological Processes:

apoptosis, inhibited
transcription, altered
transcription, induced

Kinase, in vitro:

INSR (human)
Lck (human)

Putative in vivo kinases:

Lck (human)
Src iso1 (mouse)

Regulatory protein:

Bcr (human)
LKB1 (human)

Treatment

EGF
H2O2
hypoxia
hypoxia/reoxygenation
piceatannol
PP1
siRNA
TNF
UV
vanadate

IkB-alpha (human)	DsMkDEEyEQMVkEL	Y42-p
IkB-alpha (mouse)	DsMKDEEYEQMVKEL	Y42
IkB-alpha (rat)	DsMKDEDyEQMVKEL	Y42-p

K47-ub

EEyEQMVkELQEIRL

Downstream

Effects on Modified Protein

protein degradation

Effects on Biological Processes:

transcription, induced

IkB-alpha (human)	EEyEQMVkELQEIRL	K47-ub
IkB-alpha (mouse)	EEYEQMVKELREIRL	K47
IkB-alpha (rat)	EDyEQMVKELREIRL	K47

K67-ub

PRGsEPWkQQLTEDG

Downstream

Effects on Modified Protein

activity, induced
protein degradation

IkB-alpha (human)	PRGsEPWkQQLTEDG	K67-ub
IkB-alpha (mouse)	PLAAEPWKQQLTEDG	K67
IkB-alpha (rat)	PLAAEPWKQQLTEDG	K67

K87-ub

LAIIHEEkALtMEVI

IkB-alpha (human)	LAIIHEEkALtMEVI	K87-ub
IkB-alpha (mouse)	LAIIHEEKPLTMEVI	K87
IkB-alpha (rat)	LAIIHEEKTLTMEVI	K87

T90-p

IHEEkALtMEVIRQV

IkB-alpha (human)	IHEEkALtMEVIRQV	T90-p
IkB-alpha (mouse)	IHEEKPLTMEVIGQV	T90
IkB-alpha (rat)	IHEEKTLTMEVIGQV	T90

K98-ub

MEVIRQVkGDLAFLN

IkB-alpha (human)	MEVIRQVkGDLAFLN	K98-ub
IkB-alpha (mouse)	MEVIGQVKGDLAFLN	K98
IkB-alpha (rat)	MEVIGQVKGDLAFLN	K98

S166-p

SVGVLTQsCTTPHLH

IkB-alpha (human)	SVGVLTQsCTTPHLH	S166-p
IkB-alpha (mouse)	SVAVLTQTCTPQHLH	T166
IkB-alpha (rat)	SVAVLTQTCTPQHLH	T166

K238-ub

DLVSLLLkCGADVNR

IkB-alpha (human)	DLVSLLLkCGADVNR	K238-ub
IkB-alpha (mouse)	DLVSLLLKCGADVNR	K238
IkB-alpha (rat)	DLVSLLLKCGADVNR	K238

Y248

ADVNRVTYQGYSPYQ

IkB-alpha (human)	ADVNRVTYQGYSPYQ	Y248
IkB-alpha (mouse)	ADVNRVTyQGysPYQ	Y248-p
IkB-alpha (rat)	ADVNRVTYQGYSPYQ	Y248

Y251

NRVTYQGYSPYQLTW

IkB-alpha (human)	NRVTYQGYSPYQLTW	Y251
IkB-alpha (mouse)	NRVTyQGysPYQLTW	Y251-p
IkB-alpha (rat)	NRVTYQGYSPYQLTW	Y251

S252

RVTYQGYSPYQLTWG

IkB-alpha (human)	RVTYQGYSPYQLTWG	S252
IkB-alpha (mouse)	RVTyQGysPYQLTWG	S252-p
IkB-alpha (rat)	RVTYQGYSPYQLTWG	S252

S283-p

NLQMLPEsEDEEsyD

Upstream

Downstream

Effects on Modified Protein

protein degradation

Kinase, in vitro:

CK2A1 (human)

Putative in vivo kinases:

CK2A1 (human)

IkB-alpha (human)	NLQMLPEsEDEEsyD	S283-p
IkB-alpha (mouse)	NLQMLPESEDEESYD	S283
IkB-alpha (rat)	NLQTLPESEDEESYD	S283

S288-p

PEsEDEEsyDtEsEF

Upstream

Kinase, in vitro:

CK2A1 (human)

Putative in vivo kinases:

CK2A1 (human)

IkB-alpha (human)	PEsEDEEsyDtEsEF	S288-p
IkB-alpha (mouse)	PESEDEESYDTEsEF	S288
IkB-alpha (rat)	PESEDEESYDTESEF	S288

Y289-p

EsEDEEsyDtEsEFT

Upstream

Downstream

Effects on Biological Processes:

signaling pathway regulation

Kinase, in vitro:

Btk (human)

IkB-alpha (human)	EsEDEEsyDtEsEFT	Y289-p
IkB-alpha (mouse)	ESEDEESYDTEsEFT	Y289
IkB-alpha (rat)	ESEDEESYDTESEFT	Y289

T291-p

EDEEsyDtEsEFTEF

Upstream

Downstream

Effects on Modified Protein

intracellular localization
protein degradation

Effects on Biological Processes:

carcinogenesis, induced

Kinase, in vitro:

CK2A1 (human)

Putative in vivo kinases:

CK2A1 (human)

Treatment

high_glucose

IkB-alpha (human)	EDEEsyDtEsEFTEF	T291-p
IkB-alpha (mouse)	EDEESYDTEsEFTED	T291
IkB-alpha (rat)	EDEESYDTESEFTED	T291

S293-p

EEsyDtEsEFTEFtE

Upstream

Downstream

Effects on Modified Protein

protein degradation

Kinase, in vitro:

CK2A1 (human)

Putative in vivo kinases:

CK2A1 (human)

IkB-alpha (human)	EEsyDtEsEFTEFtE	S293-p
IkB-alpha (mouse)	EESYDTEsEFTEDEL	S293-p
IkB-alpha (rat)	EESYDTESEFTEDEL	S293

T299-p

EsEFTEFtEDELPyD

Upstream

Downstream

Effects on Modified Protein

protein degradation

Kinase, in vitro:

CK2A1 (human)

IkB-alpha (human)	EsEFTEFtEDELPyD	T299-p
IkB-alpha (mouse)	gap	-
IkB-alpha (rat)	gap	-

Y305-p

FtEDELPyDDCVFGG

Upstream

Downstream

Effects on Modified Protein

activity, inhibited
intracellular localization
protein degradation
protein stabilization

Effects on Biological Processes:

apoptosis, induced
signaling pathway regulation
transcription, inhibited

Kinase, in vitro:

Btk (human)

Putative in vivo kinases:

Abl (human)
Btk (human)

Regulatory protein:

Bcr (human)

IkB-alpha (human)	FtEDELPyDDCVFGG	Y305-p
IkB-alpha (mouse)	FTEDELPYDDCVFGG	Y302
IkB-alpha (rat)	FTEDELPYDDCVFGG	Y302

S63-p

PQEVPRGsEPWkQQL

Upstream

Downstream

Effects on Modified Protein

phosphorylation

Effects on Biological Processes:

apoptosis, inhibited
carcinogenesis, induced
cell growth, induced
transcription, induced

Kinase, in vitro:

AurA (human)
AurB (human)
AurC (human)

Putative in vivo kinases:

AurC (human)

IkB-alpha (human)	PQEVPRGsEPWkQQL	S63-p
IkB-alpha (mouse)	PQEAPLAAEPWKQQL	A63
IkB-alpha (rat)	PQEAPLAAEPWKQQL	A63

S262-p

QLTWGRPsTRIQQQL

Upstream

Downstream

Effects on Modified Protein

phosphorylation

Effects on Biological Processes:

apoptosis, inhibited
carcinogenesis, induced
cell growth, induced
transcription, induced

Kinase, in vitro:

AurA (human)
AurB (human)
AurC (human)

Putative in vivo kinases:

AurC (human)

IkB-alpha (human)	QLTWGRPsTRIQQQL	S262-p
IkB-alpha (mouse)	QLTWGRPSTRIQQQL	S262
IkB-alpha (rat)	QLTWGRPSTRIQQQL	S262