HSP27 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

R5-m1

___MTERrVPFsLLr

HSP27 (human)	___MTERrVPFsLLr	R5-m1
HSP27 (mouse)	___MTERRVPFsLLr	R5
HSP27 (rat)	___MTERRVPFsLLR	R5
HSP27 (pig)	___MTERRVPFSLLR	R5
HSP27 (hamster)	___MTERRVPFSLLR	R5
HSP27 (chicken)	___MAERRVPFTFLT	R5
HSP27 (dog)	___MTERRVPFSLLR	R5
HSP27 (cow)	___MAERRVPFSLLR	R5

S9-p

TERrVPFsLLrGPsW

HSP27 (human)	TERrVPFsLLrGPsW	S9-p
HSP27 (mouse)	TERRVPFsLLrsPsW	S9-p
HSP27 (rat)	TERRVPFsLLRsPsW	S9-p
HSP27 (pig)	TERRVPFSLLRSPSW	S9
HSP27 (hamster)	TERRVPFSLLRSPsW	S9
HSP27 (chicken)	AERRVPFTFLTSPsW	T9
HSP27 (dog)	TERRVPFSLLRSPsW	S9
HSP27 (cow)	AERRVPFSLLRGPSW	S9

R12-m1

rVPFsLLrGPsWDPF

HSP27 (human)	rVPFsLLrGPsWDPF	R12-m1
HSP27 (mouse)	RVPFsLLrsPsWEPF	R12-m1
HSP27 (rat)	RVPFsLLRsPsWEPF	R12
HSP27 (pig)	RVPFSLLRSPSWDPF	R12
HSP27 (hamster)	RVPFSLLRSPsWEPF	R12
HSP27 (chicken)	RVPFTFLTSPsWEPF	T12
HSP27 (dog)	RVPFSLLRSPsWDPF	R12
HSP27 (cow)	RVPFSLLRGPSWDPF	R12

G13

VPFsLLrGPsWDPFr

HSP27 (human)	VPFsLLrGPsWDPFr	G13
HSP27 (mouse)	VPFsLLrsPsWEPFR	S13-p
HSP27 (rat)	VPFsLLRsPsWEPFR	S13-p
HSP27 (pig)	VPFSLLRSPSWDPFR	S13
HSP27 (hamster)	VPFSLLRSPsWEPFR	S13
HSP27 (chicken)	VPFTFLTSPsWEPFR	S13
HSP27 (dog)	VPFSLLRSPsWDPFR	S13
HSP27 (cow)	VPFSLLRGPSWDPFR	G13

S15-p

FsLLrGPsWDPFrDW

Upstream

Downstream

111

HSP27 (human)
S15-p

2404 : Phospho-HSP27 (Ser15) Antibody

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein conformation
protein degradation
protein stabilization
sumoylation

Effects on Biological Processes:

apoptosis, altered
apoptosis, inhibited
autophagy, induced
autophagy, inhibited
carcinogenesis, induced
cell growth, altered
cell growth, induced
cytoskeletal reorganization
signaling pathway regulation
transcription, altered
transcription, inhibited

Kinase, in vitro:

MAPKAPK2 (human)
PKG1 iso2 (human)

Putative in vivo kinases:

MAPKAPK2 (human)
PKCA (human)

Regulatory protein:

CD38 (human)
FAM83G (human)
MAPKAPK3 (human)
P38A (no information)

Treatment

adriamycin
ADT
arsenite
ATI-450
bacterial infection
CID755673
circRNA
cisplatin
D3T
dasatinib
EGF
FIRINOX
fluorouracil
Go_6983
heat_shock
heating
histamine
IL-1b
ionizing_radiation
ischemia
J2
lapatinib
LLOMe
LRRK2-IN-1
LY2228820
Lys05
menadione
MG132_withdrawal
MK2-I
mutation
OSU-03012
PAR1-activating_peptide
PF3644022
SB202190
SB203580
siRNA
SN-38
thiolutin
thrombin
trastuzumab
U0126
vorapaxar
ZK-Thiazolidinone

HSP27 (human)	FsLLrGPsWDPFrDW	S15-p
HSP27 (mouse)	FsLLrsPsWEPFRDW	S15-p
HSP27 (rat)	FsLLRsPsWEPFRDW	S15-p
HSP27 (pig)	FSLLRSPSWDPFRDW	S15
HSP27 (hamster)	FSLLRSPsWEPFRDW	S15-p
HSP27 (chicken)	FTFLTSPsWEPFRDW	S15-p
HSP27 (dog)	FSLLRSPsWDPFRDW	S15-p
HSP27 (cow)	FSLLRGPSWDPFRDW	S15

R20-m1

GPsWDPFrDWyPHsR

HSP27 (human)	GPsWDPFrDWyPHsR	R20-m1
HSP27 (mouse)	sPsWEPFRDWYPAHS	R20
HSP27 (rat)	sPsWEPFRDWYPAHs	R20
HSP27 (pig)	SPSWDPFRDWYPAHS	R20
HSP27 (hamster)	SPsWEPFRDWYPAHS	R20
HSP27 (chicken)	SPsWEPFRDWYHGSR	R20
HSP27 (dog)	SPsWDPFRDWYPAHS	R20
HSP27 (cow)	GPSWDPFRDWYPAHS	R20

Y23-p

WDPFrDWyPHsRLFD

HSP27 (human)	WDPFrDWyPHsRLFD	Y23-p
HSP27 (mouse)	WEPFRDWYPAHSRLF	Y23
HSP27 (rat)	WEPFRDWYPAHsRLF	Y23
HSP27 (pig)	WDPFRDWYPAHSRLF	Y23
HSP27 (hamster)	WEPFRDWYPAHSRLF	Y23
HSP27 (chicken)	WEPFRDWYHGSRLFD	Y23
HSP27 (dog)	WDPFRDWYPAHSRLF	Y23
HSP27 (cow)	WDPFRDWYPAHSRLF	Y23

S26-p

FrDWyPHsRLFDQAF

HSP27 (human)	FrDWyPHsRLFDQAF	S26-p
HSP27 (mouse)	RDWYPAHSRLFDQAF	S27
HSP27 (rat)	RDWYPAHsRLFDQAF	S27-p
HSP27 (pig)	RDWYPAHSRLFDQAF	S27
HSP27 (hamster)	RDWYPAHSRLFDQAF	S27
HSP27 (chicken)	FRDWYHGSRLFDQSF	S26
HSP27 (dog)	RDWYPAHSRLFDQAF	S27
HSP27 (cow)	RDWYPAHSRLFDQAF	S27

R37-m1

DQAFGLPrLPEEWSQ

HSP27 (human)	DQAFGLPrLPEEWSQ	R37-m1
HSP27 (mouse)	DQAFGVPRLPDEWSQ	R38
HSP27 (rat)	DQAFGVPrFPDEWSQ	R38-m1
HSP27 (pig)	DQAFGLPRLPEEWSQ	R38
HSP27 (hamster)	DQAFGVPRLPDEWSQ	R38
HSP27 (chicken)	DQSFGMPHIPEDWYK	H37
HSP27 (dog)	DQAFGLPRLPEEWAQ	R38
HSP27 (cow)	DQAFGLPRLPEEWSQ	R38

Y54-p

GGSSWPGyVrPLPPA

HSP27 (human)	GGSSWPGyVrPLPPA	Y54-p
HSP27 (mouse)	SAAGWPGYVRPLPAA	Y55
HSP27 (rat)	SSAGWPGYVRPLPAA	Y55
HSP27 (pig)	SHSGWPGYVRPLPPP	Y55
HSP27 (hamster)	SAAGWPGYVRPLPAA	Y55
HSP27 (chicken)	SGSAWPGYFRLLPSE	Y54
HSP27 (dog)	GHSGWPGYVRPIPPA	Y55
HSP27 (cow)	SHSGWPGYVRALPAA	Y55

R56-m1

SSWPGyVrPLPPAAI

HSP27 (human)	SSWPGyVrPLPPAAI	R56-m1
HSP27 (mouse)	AGWPGYVRPLPAATA	R57
HSP27 (rat)	AGWPGYVRPLPAATA	R57
HSP27 (pig)	SGWPGYVRPLPPPAI	R57
HSP27 (hamster)	AGWPGYVRPLPAATA	R57
HSP27 (chicken)	SAWPGYFRLLPSESA	R56
HSP27 (dog)	SGWPGYVRPIPPAVE	R57
HSP27 (cow)	SGWPGYVRALPAAAI	R57

S65-p

LPPAAIEsPAVAAPA

Upstream

Regulatory protein:

CD38 (human)

Treatment

LRRK2-IN-1
nocodazole
selumetinib
vemurafenib
ZK-Thiazolidinone

HSP27 (human)	LPPAAIEsPAVAAPA	S65-p
HSP27 (mouse)	LPAATAEGPAAVtLA	G66
HSP27 (rat)	LPAATAEGPAAVTLA	G66
HSP27 (pig)	LPPPAIEGPAAVAAP	G66
HSP27 (hamster)	LPAATAEGPAAVALA	G66
HSP27 (chicken)	GYFRLLPSESALLPA	S60
HSP27 (dog)	PIPPAVEGPAAAAAA	G65
HSP27 (cow)	gap	-

A67

PAAIEsPAVAAPAys

HSP27 (human)	PAAIEsPAVAAPAys	A67
HSP27 (mouse)	AEGPAAVtLAAPAFs	T71-p
HSP27 (rat)	AEGPAAVTLARPAFS	T71
HSP27 (pig)	gap	-
HSP27 (hamster)	AEGPAAVALAAPLAA	A71
HSP27 (chicken)	LPSESALLPAPGSPY	L65
HSP27 (dog)	VEGPAAAAAAAAPAY	A70
HSP27 (cow)	gap	-

Y73-p

PAVAAPAysRALsRQ

HSP27 (human)	PAVAAPAysRALsRQ	Y73-p
HSP27 (mouse)	VtLAAPAFsRALNRQ	F77
HSP27 (rat)	VTLARPAFSRALNRQ	F77
HSP27 (pig)	AAVAAPAYSRLLSRQ	Y75
HSP27 (hamster)	APLAAPAFHRALNRQ	F81
HSP27 (chicken)	LPAPGSPYGRALsEL	Y72
HSP27 (dog)	AAAAAPAYsRALSRQ	Y77
HSP27 (cow)	AAIEGPAYNRALSRQ	Y69

S74-p

AVAAPAysRALsRQL

HSP27 (human)	AVAAPAysRALsRQL	S74-p
HSP27 (mouse)	tLAAPAFsRALNRQL	S78-p
HSP27 (rat)	TLARPAFSRALNRQL	S78
HSP27 (pig)	AVAAPAYSRLLSRQL	S76
HSP27 (hamster)	PLAAPAFHRALNRQL	H82
HSP27 (chicken)	PAPGSPYGRALsELS	G73
HSP27 (dog)	AAAAPAYsRALSRQL	S78-p
HSP27 (cow)	AIEGPAYNRALSRQL	N70

S78-p

PAysRALsRQLssGV

Upstream

Downstream

HSP27 (human)
S78-p

Effects on Modified Protein

activity, induced
enzymatic activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein conformation
protein degradation
sumoylation
ubiquitination

Effects on Biological Processes:

apoptosis, altered
apoptosis, inhibited
autophagy, induced
autophagy, inhibited
carcinogenesis, induced
cell cycle regulation
cell differentiation, induced
cell growth, altered
cell growth, induced
cell motility, induced
cytoskeletal reorganization
signaling pathway regulation
transcription, altered
transcription, inhibited

Kinase, in vitro:

MAPKAPK2 (human)
PKACA (human)
PKG1 iso2 (human)

Putative in vivo kinases:

MAPKAPK2 (human)
P70S6KB (human)

Regulatory protein:

AR (human)
BMP10 (human)
CD38 (human)
CLN3 (human)
CLPB (human)
DSG3 (human)
GADD45B (human)
GDF2 (human)
HAX1 (human)
MAPKAPK2 (human)
MAPKAPK3 (human)
P38A (human)
P38A (no information)
SMAD4 (human)

Treatment

5-iodotubercidin
ACh
actinomycin_D
adriamycin
ADT
ara-a
arsenite
ATI-450
bacterial infection
Bay61-3606
bicalutamide
BIRB-0796
calyculin_A
CID755673
circRNA
cisplatin
collagen
CXCL12
D3T
diclofenac
DRB
FGF2
FIRINOX
FLUVOXAMINE_MALEATE
Go_6983
H2O2
HA-1077
heat_shock
heating
heregulin
histamine
IL-1b
J2
LLOMe
LY2228820
menadione
metribolone
mutation
nerve_damage
nocodazole
NSC23766
OSU-03012
PAR1-activating_peptide
PD98059
PF3622044
PF3644022
phorbol_ester
S-diclofenac
S-sulindac
S-valproate
SB202190
SB203347
SB203580
serum
siRNA
SN-38
sodium_hydrosulfide
SP600125
spermine-NONOate
sulindac
taxol
thiolutin
thrombin
TNF
Tramadol
trastuzumab
UVB
valproic acid
vorapaxar
Y27632

HSP27 (human)	PAysRALsRQLssGV	S78-p
HSP27 (mouse)	PAFsRALNRQLssGV	N82
HSP27 (rat)	PAFSRALNRQLssGV	N82
HSP27 (pig)	PAYSRLLSRQLsSGV	S80
HSP27 (hamster)	PAFHRALNRQLsSGV	N86
HSP27 (chicken)	SPYGRALsELSsGIS	S77-p
HSP27 (dog)	PAYsRALSRQLsSGV	S82
HSP27 (cow)	PAYNRALSRQLsSGV	S74

S82-p

RALsRQLssGVsEIr

Upstream

Downstream

395

HSP27 (human)
S82-p

HSP27 (mouse)
S86-p

HSP27 (rat)
S86-p

HSP27 (hamster)
S90-p

HSP27 (dog)
S86-p

Effects on Modified Protein

activity, induced
enzymatic activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein conformation
protein degradation
sumoylation
ubiquitination

Effects on Biological Processes:

apoptosis, altered
apoptosis, inhibited
autophagy, induced
autophagy, inhibited
carcinogenesis, induced
cell differentiation, induced
cell growth, altered
cell growth, induced
cell motility, induced
cytoskeletal reorganization
signaling pathway regulation
transcription, altered
transcription, inhibited

Kinase, in vitro:

Akt1 (human)
MAPKAPK2 (human)
PKACA (human)
PKG1 iso2 (human)
PRKD1 (human)

Putative in vivo kinases:

Akt1 (human)
MAPKAPK2 (human)
MAPKAPK3 (human)
P70S6KB (human)
PRKD1 (human)

Regulatory protein:

ANXA1 (human)
AR (human)
CD38 (human)
Chk1 (human)
CLN3 (human)
CLPB (human)
CYBA (human)
DSG3 (human)
FAM83G (human)
HAX1 (human)
IKKA (human)
MAPKAPK2 (human)
MKK3 (human)
MKK6 (human)
MLC1 (human)
P38A (human)
PRKD1 (human)
SMAD4 (human)
SPARC (human)
TNF-R1 (human)
TNF-R2 (human)

Treatment

(5Z)-7-oxozeaenol
ACh
adriamycin
ADT
angiotensin-(1-7)
anisomycin
apocynin
arsenite
ATI-450
BI605906
bicalutamide
BIRB-0796
bombesin
bryostatin_1
Candidalysin
CID755673
circRNA
cisplatin
CRT0066101
D3T
dasatinib
EGF
extract
FGF2
FIRINOX
fluorouracil
gefitinib
Go_6976
Go_6983
GSK963
H2O2
heat_shock
heating
heme
heregulin
histamine
IL-1b
ionizing_radiation
ischemia
J2
lapatinib
LLOMe
LPA
LY2228820
Lys05
menadione
metastatic potential
metribolone
MG132_withdrawal
microcystin-LR
MK2-I
mutation
NAC
neurotensin
nocodazole
OSU-03012
oxaliplatin
PAR1-activating_peptide
PD98059
PF-3604422
PF3644022
phorbol_ester
PP1
Ro6842262
salicylaldiminato)Pt(II) complex with dimethylpropylene linkage
SB202190
SB203347
SB203580
serum
siRNA
SN-38
sorbitol
SP600125
taxol
tert-butylhydroperoxide
thiolutin
thrombin
TNF
U0126
UCN-01
UV
UVB
vorapaxar
WKYMVm
Y27632

HSP27 (human)	RALsRQLssGVsEIr	S82-p
HSP27 (mouse)	RALNRQLssGVsEIR	S86-p
HSP27 (rat)	RALNRQLssGVsEIR	S86-p
HSP27 (pig)	RLLSRQLsSGVSEIQ	S84-p
HSP27 (hamster)	RALNRQLsSGVSEIR	S90-p
HSP27 (chicken)	GRALsELSsGISEIR	S80
HSP27 (dog)	RALSRQLsSGVSEIR	S86-p
HSP27 (cow)	RALSRQLsSGVSEIQ	S78-p

S83-p

ALsRQLssGVsEIrH

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

cell differentiation, induced

Regulatory protein:

CD38 (human)
MS4A3 (human)

Treatment

H2O2
ionizing_radiation
metformin
NaB
T-2 toxin

HSP27 (human)	ALsRQLssGVsEIrH	S83-p
HSP27 (mouse)	ALNRQLssGVsEIRQ	S87-p
HSP27 (rat)	ALNRQLssGVsEIRQ	S87-p
HSP27 (pig)	LLSRQLsSGVSEIQQ	S85
HSP27 (hamster)	ALNRQLsSGVSEIRQ	S91
HSP27 (chicken)	RALsELSsGISEIRQ	S81-p
HSP27 (dog)	ALSRQLsSGVSEIRQ	S87
HSP27 (cow)	ALSRQLsSGVSEIQQ	S79

S86-p

RQLssGVsEIrHtAD

HSP27 (human)	RQLssGVsEIrHtAD	S86-p
HSP27 (mouse)	RQLssGVsEIRQTAD	S90-p
HSP27 (rat)	RQLssGVsEIRQTAD	S90-p
HSP27 (pig)	RQLsSGVSEIQQTAD	S88
HSP27 (hamster)	RQLsSGVSEIRQTAD	S94
HSP27 (chicken)	sELSsGISEIRQSAD	S84
HSP27 (dog)	RQLsSGVSEIRQTAD	S90
HSP27 (cow)	RQLsSGVSEIQQTAD	S82

R89-m1

ssGVsEIrHtADRWR

HSP27 (human)	ssGVsEIrHtADRWR	R89-m1
HSP27 (mouse)	ssGVsEIRQTADRWR	R93
HSP27 (rat)	ssGVsEIRQTADRWR	R93
HSP27 (pig)	sSGVSEIQQTADRWR	Q91
HSP27 (hamster)	sSGVSEIRQTADRWR	R97
HSP27 (chicken)	SsGISEIRQSADSWK	R87
HSP27 (dog)	sSGVSEIRQTADRWR	R93
HSP27 (cow)	sSGVSEIQQTADRWR	Q85

T91-p

GVsEIrHtADRWRVs

Upstream

Regulatory protein:

CD38 (human)

HSP27 (human)	GVsEIrHtADRWRVs	T91-p
HSP27 (mouse)	GVsEIRQTADRWRVS	T95
HSP27 (rat)	GVsEIRQTADRWRVs	T95
HSP27 (pig)	GVSEIQQTADRWRVS	T93
HSP27 (hamster)	GVSEIRQTADRWRVS	T99
HSP27 (chicken)	GISEIRQSADSWKVT	S89
HSP27 (dog)	GVSEIRQTADRWRVS	T95
HSP27 (cow)	GVSEIQQTADRWRVS	T87

S98-p

tADRWRVsLDVNHFA

HSP27 (human)	tADRWRVsLDVNHFA	S98-p
HSP27 (mouse)	TADRWRVSLDVNHFA	S102
HSP27 (rat)	TADRWRVsLDVNHFA	S102-p
HSP27 (pig)	TADRWRVSLDVNHFA	S100
HSP27 (hamster)	TADRWRVSLDVNHFA	S106
HSP27 (chicken)	SADSWKVTLDVNHFA	T96
HSP27 (dog)	TADRWRVSLDVNHFA	S102
HSP27 (cow)	TADRWRVSLDVNHFA	S94

K112-ub

APDELTVktkDGVVE

HSP27 (human)	APDELTVktkDGVVE	K112-ub
HSP27 (mouse)	APEELTVkTkEGVVE	K116-ub
HSP27 (rat)	APEELTVKtKEGVVE	K116
HSP27 (pig)	APEELTVKTKDGVVE	K114
HSP27 (hamster)	APEELTVKTKEGVVE	K120
HSP27 (chicken)	APEELVVKTKDNIVE	K110
HSP27 (dog)	APEELTVKTKDGVVE	K116
HSP27 (cow)	APEELTVKTKDGVVE	K108

T113-p

PDELTVktkDGVVEI

HSP27 (human)	PDELTVktkDGVVEI	T113-p
HSP27 (mouse)	PEELTVkTkEGVVEI	T117
HSP27 (rat)	PEELTVKtKEGVVEI	T117-p
HSP27 (pig)	PEELTVKTKDGVVEI	T115
HSP27 (hamster)	PEELTVKTKEGVVEI	T121
HSP27 (chicken)	PEELVVKTKDNIVEI	T111
HSP27 (dog)	PEELTVKTKDGVVEI	T117
HSP27 (cow)	PEELTVKTKDGVVEI	T109

K114-ub

DELTVktkDGVVEIt

HSP27 (human)	DELTVktkDGVVEIt	K114-ub
HSP27 (mouse)	EELTVkTkEGVVEIT	K118-ub
HSP27 (rat)	EELTVKtKEGVVEIT	K118
HSP27 (pig)	EELTVKTKDGVVEIT	K116
HSP27 (hamster)	EELTVKTKEGVVEIT	K122
HSP27 (chicken)	EELVVKTKDNIVEIT	K112
HSP27 (dog)	EELTVKTKDGVVEIT	K118
HSP27 (cow)	EELTVKTKDGVVEIT	K110

K114-sm

DELTVktkDGVVEIt

HSP27 (human)	DELTVktkDGVVEIt	K114-sm
HSP27 (mouse)	EELTVkTKEGVVEIT	K118
HSP27 (rat)	EELTVKtKEGVVEIT	K118
HSP27 (pig)	EELTVKTKDGVVEIT	K116
HSP27 (hamster)	EELTVKTKEGVVEIT	K122
HSP27 (chicken)	EELVVKTKDNIVEIT	K112
HSP27 (dog)	EELTVKTKDGVVEIT	K118
HSP27 (cow)	EELTVKTKDGVVEIT	K110

T121-p

kDGVVEItGkHEERQ

HSP27 (human)	kDGVVEItGkHEERQ	T121-p
HSP27 (mouse)	kEGVVEITGKHEERQ	T125
HSP27 (rat)	KEGVVEITGkHEERQ	T125
HSP27 (pig)	KDGVVEITGKHEERQ	T123
HSP27 (hamster)	KEGVVEITGKHEERQ	T129
HSP27 (chicken)	KDNIVEITGKHEEKQ	T119
HSP27 (dog)	KDGVVEITGKHEERQ	T125
HSP27 (cow)	KDGVVEITGKHEERQ	T117

K123-ac

GVVEItGkHEERQDE

Upstream

Regulatory protein:

ING5 (human)

Treatment

vorinostat

HSP27 (human)	GVVEItGkHEERQDE	K123-ac
HSP27 (mouse)	GVVEITGKHEERQDE	K127
HSP27 (rat)	GVVEITGkHEERQDE	K127-ac
HSP27 (pig)	GVVEITGKHEERQDE	K125
HSP27 (hamster)	GVVEITGKHEERQDE	K131
HSP27 (chicken)	NIVEITGKHEEKQDE	K121
HSP27 (dog)	GVVEITGKHEERQDE	K127
HSP27 (cow)	GVVEITGKHEERQDE	K119

K123-ub

GVVEItGkHEERQDE

HSP27 (human)	GVVEItGkHEERQDE	K123-ub
HSP27 (mouse)	GVVEITGKHEERQDE	K127
HSP27 (rat)	GVVEITGKHEERQDE	K127
HSP27 (pig)	GVVEITGKHEERQDE	K125
HSP27 (hamster)	GVVEITGKHEERQDE	K131
HSP27 (chicken)	NIVEITGKHEEKQDE	K121
HSP27 (dog)	GVVEITGKHEERQDE	K127
HSP27 (cow)	GVVEITGKHEERQDE	K119

Y133-p

ERQDEHGyIsRCFtR

HSP27 (human)	ERQDEHGyIsRCFtR	Y133-p
HSP27 (mouse)	ERQDEHGYIsRCFTR	Y137
HSP27 (rat)	ERQDEHGYISRCFTR	Y137
HSP27 (pig)	ERQDEHGFISRCFTR	F135
HSP27 (hamster)	ERQDEHGYISRCFTR	Y141
HSP27 (chicken)	EKQDEHGFISRCFTR	F131
HSP27 (dog)	ERQDEHGYISRRLTP	Y137
HSP27 (cow)	ERQDEHGYISRCFTR	Y129

S135-p

QDEHGyIsRCFtRky

Upstream

Treatment

EGF
U0126

HSP27 (human)	QDEHGyIsRCFtRky	S135-p
HSP27 (mouse)	QDEHGYIsRCFTRkY	S139-p
HSP27 (rat)	QDEHGYISRCFTRKY	S139
HSP27 (pig)	QDEHGFISRCFTRKY	S137
HSP27 (hamster)	QDEHGYISRCFTRKY	S143
HSP27 (chicken)	QDEHGFISRCFTRKY	S133
HSP27 (dog)	QDEHGYISRRLTPKY	S139
HSP27 (cow)	QDEHGYISRCFTRKY	S131

T139-p

GyIsRCFtRkytLPP

HSP27 (human)	GyIsRCFtRkytLPP	T139-p
HSP27 (mouse)	GYIsRCFTRkYtLPP	T143
HSP27 (rat)	GYISRCFTRKYtLPP	T143
HSP27 (pig)	GFISRCFTRKYTLPP	T141
HSP27 (hamster)	GYISRCFTRKYTLPP	T147
HSP27 (chicken)	GFISRCFTRKYTLPP	T137
HSP27 (dog)	GYISRRLTPKYTLPP	T143
HSP27 (cow)	GYISRCFTRKYTLPP	T135

K141-ub

IsRCFtRkytLPPGV

HSP27 (human)	IsRCFtRkytLPPGV	K141-ub
HSP27 (mouse)	IsRCFTRkYtLPPGV	K145-ub
HSP27 (rat)	ISRCFTRKYtLPPGV	K145
HSP27 (pig)	ISRCFTRKYTLPPGV	K143
HSP27 (hamster)	ISRCFTRKYTLPPGV	K149
HSP27 (chicken)	ISRCFTRKYTLPPGV	K139
HSP27 (dog)	ISRRLTPKYTLPPGV	K145
HSP27 (cow)	ISRCFTRKYTLPPGV	K137

K141-ac

IsRCFtRkytLPPGV

HSP27 (human)	IsRCFtRkytLPPGV	K141-ac
HSP27 (mouse)	IsRCFTRKYtLPPGV	K145
HSP27 (rat)	ISRCFTRKYtLPPGV	K145
HSP27 (pig)	ISRCFTRKYTLPPGV	K143
HSP27 (hamster)	ISRCFTRKYTLPPGV	K149
HSP27 (chicken)	ISRCFTRKYTLPPGV	K139
HSP27 (dog)	ISRRLTPKYTLPPGV	K145
HSP27 (cow)	ISRCFTRKYTLPPGV	K137

Y142-p

sRCFtRkytLPPGVD

HSP27 (human)	sRCFtRkytLPPGVD	Y142-p
HSP27 (mouse)	sRCFTRkYtLPPGVD	Y146
HSP27 (rat)	SRCFTRKYtLPPGVD	Y146
HSP27 (pig)	SRCFTRKYTLPPGVD	Y144
HSP27 (hamster)	SRCFTRKYTLPPGVD	Y150
HSP27 (chicken)	SRCFTRKYTLPPGVE	Y140
HSP27 (dog)	SRRLTPKYTLPPGVD	Y146
HSP27 (cow)	SRCFTRKYTLPPGVD	Y138

T143-p

RCFtRkytLPPGVDP

Upstream

Kinase, in vitro:

PKACA (human)
PKG1 iso2 (human)

HSP27 (human)	RCFtRkytLPPGVDP	T143-p
HSP27 (mouse)	RCFTRkYtLPPGVDP	T147-p
HSP27 (rat)	RCFTRKYtLPPGVDP	T147-p
HSP27 (pig)	RCFTRKYTLPPGVDP	T145
HSP27 (hamster)	RCFTRKYTLPPGVDP	T151
HSP27 (chicken)	RCFTRKYTLPPGVEA	T141
HSP27 (dog)	RRLTPKYTLPPGVDP	T147
HSP27 (cow)	RCFTRKYTLPPGVDP	T139

T162-p

SSLSPEGtLtVEAPM

HSP27 (human)	SSLSPEGtLtVEAPM	T162-p
HSP27 (mouse)	SSLSPEGTLTVEAPL	T166
HSP27 (rat)	SSLSPEGTLTVEAPL	T166
HSP27 (pig)	SSLSPEGTLSVEAPL	T164
HSP27 (hamster)	SSLSPEGTLTVEAPL	T170
HSP27 (chicken)	SSLSPDGMLTVEAPL	M160
HSP27 (dog)	SSLSPEGTLTVEAPM	T166
HSP27 (cow)	SSLSPEGTLTVEAPL	T158

T164-p

LSPEGtLtVEAPMPk

HSP27 (human)	LSPEGtLtVEAPMPk	T164-p
HSP27 (mouse)	LSPEGTLTVEAPLPK	T168
HSP27 (rat)	LSPEGTLTVEAPLPK	T168
HSP27 (pig)	LSPEGTLSVEAPLPK	S166
HSP27 (hamster)	LSPEGTLTVEAPLPK	T172
HSP27 (chicken)	LSPDGMLTVEAPLPK	T162
HSP27 (dog)	LSPEGTLTVEAPMPK	T168
HSP27 (cow)	LSPEGTLTVEAPLPK	T160

K171-ub

tVEAPMPkLAtQsNE

HSP27 (human)	tVEAPMPkLAtQsNE	K171-ub
HSP27 (mouse)	TVEAPLPKAVTQsAE	K175
HSP27 (rat)	TVEAPLPKAVTQSAE	K175
HSP27 (pig)	SVEAPLPKPATQSAE	K173
HSP27 (hamster)	TVEAPLPKTATQSAE	K179
HSP27 (chicken)	TVEAPLPKPAIQSSE	K169
HSP27 (dog)	TVEAPMPKPATQSAE	K175
HSP27 (cow)	TVEAPLPKSATQSAE	K167

T174-p

APMPkLAtQsNEItI

HSP27 (human)	APMPkLAtQsNEItI	T174-p
HSP27 (mouse)	APLPKAVTQsAEITI	T178
HSP27 (rat)	APLPKAVTQSAEITI	T178
HSP27 (pig)	APLPKPATQSAEITI	T176
HSP27 (hamster)	APLPKTATQSAEITI	T182
HSP27 (chicken)	APLPKPAIQSSEITI	I172
HSP27 (dog)	APMPKPATQSAEITI	T178
HSP27 (cow)	APLPKSATQSAEITI	T170

S176-p

MPkLAtQsNEItIPV

Upstream

Kinase, in vitro:

P38A (human)

Treatment

ischemia

HSP27 (human)	MPkLAtQsNEItIPV	S176-p
HSP27 (mouse)	LPKAVTQsAEITIPV	S180-p
HSP27 (rat)	LPKAVTQSAEITIPV	S180
HSP27 (pig)	LPKPATQSAEITIPV	S178
HSP27 (hamster)	LPKTATQSAEITIPV	S184
HSP27 (chicken)	LPKPAIQSSEITIPV	S174
HSP27 (dog)	MPKPATQSAEITIPV	S180
HSP27 (cow)	LPKSATQSAEITIPV	S172

T180-p

AtQsNEItIPVtFEs

HSP27 (human)	AtQsNEItIPVtFEs	T180-p
HSP27 (mouse)	VTQsAEITIPVTFEA	T184
HSP27 (rat)	VTQSAEITIPVTFEA	T184
HSP27 (pig)	ATQSAEITIPVTFEA	T182
HSP27 (hamster)	ATQSAEITIPVTFEA	T188
HSP27 (chicken)	AIQSSEITIPVTVEA	T178
HSP27 (dog)	ATQSAEITIPVTFEA	T184
HSP27 (cow)	ATQSAEITIPVTFQA	T176

T184-p

NEItIPVtFEsRAQL

HSP27 (human)	NEItIPVtFEsRAQL	T184-p
HSP27 (mouse)	AEITIPVTFEARAQI	T188
HSP27 (rat)	AEITIPVTFEARAQI	T188
HSP27 (pig)	AEITIPVTFEARAQL	T186
HSP27 (hamster)	AEITIPVTFEARAQI	T192
HSP27 (chicken)	SEITIPVTVEAKKEE	T182
HSP27 (dog)	AEITIPVTFEARAQI	T188
HSP27 (cow)	AEITIPVTFQARAQL	T180

S187-p

tIPVtFEsRAQLGGP

HSP27 (human)	tIPVtFEsRAQLGGP	S187-p
HSP27 (mouse)	TIPVTFEARAQIGGP	A191
HSP27 (rat)	TIPVTFEARAQIGGP	A191
HSP27 (pig)	TIPVTFEARAQLGGT	A189
HSP27 (hamster)	TIPVTFEARAQIGGQ	A195
HSP27 (chicken)	gap	-
HSP27 (dog)	TIPVTFEARAQIGGP	A191
HSP27 (cow)	TIPVTFQARAQLGGP	A183

K198-ub

LGGPEAAksDEtAAk

HSP27 (human)	LGGPEAAksDEtAAk	K198-ub
HSP27 (mouse)	IGGPEAGksEQsGAK	K202-ub
HSP27 (rat)	gap	-
HSP27 (pig)	LGGTEAGKSEKPGTK	K200
HSP27 (hamster)	IGGQEAGKSEQSGAK	K206
HSP27 (chicken)	IPVTVEAKKEEPAKK	K186
HSP27 (dog)	IGGPEAGKSEQSGAK	K202
HSP27 (cow)	LGGPEAGKSEQPENK	K194

K198-sm

LGGPEAAksDEtAAk

HSP27 (human)	LGGPEAAksDEtAAk	K198-sm
HSP27 (mouse)	IGGPEAGKsEQsGAK	K202
HSP27 (rat)	gap	-
HSP27 (pig)	LGGTEAGKSEKPGTK	K200
HSP27 (hamster)	IGGQEAGKSEQSGAK	K206
HSP27 (chicken)	IPVTVEAKKEEPAKK	K186
HSP27 (dog)	IGGPEAGKSEQSGAK	K202
HSP27 (cow)	LGGPEAGKSEQPENK	K194

S199-p

GGPEAAksDEtAAk_

Upstream

Regulatory protein:

CD38 (human)

Treatment

EGF
metformin
ZK-Thiazolidinone

HSP27 (human)	GGPEAAksDEtAAk_	S199-p
HSP27 (mouse)	GGPEAGksEQsGAK_	S203-p
HSP27 (rat)	AQIGGPESEQSGAK_	S200
HSP27 (pig)	GGTEAGKSEKPGTK_	S201
HSP27 (hamster)	GGQEAGKSEQSGAK_	S207
HSP27 (chicken)	PVTVEAKKEEPAKK_	K187
HSP27 (dog)	GGPEAGKSEQSGAK_	S203
HSP27 (cow)	GGPEAGKSEQPENK_	S195

T202-p

EAAksDEtAAk____

Upstream

Treatment

metformin

HSP27 (human)	EAAksDEtAAk____	T202-p
HSP27 (mouse)	EAGksEQsGAK____	S206-p
HSP27 (rat)	GGPESEQSGAK____	S203
HSP27 (pig)	EAGKSEKPGTK____	P204
HSP27 (hamster)	EAGKSEQSGAK____	S210
HSP27 (chicken)	VEAKKEEPAKK____	P190
HSP27 (dog)	EAGKSEQSGAK____	S206
HSP27 (cow)	EAGKSEQPENK____	P198

K205-ac

ksDEtAAk_______

HSP27 (human)	ksDEtAAk_______	K205-ac
HSP27 (mouse)	ksEQsGAK_______	K209
HSP27 (rat)	ESEQSGAK_______	K206
HSP27 (pig)	KSEKPGTK_______	K207
HSP27 (hamster)	KSEQSGAK_______	K213
HSP27 (chicken)	KKEEPAKK_______	K193
HSP27 (dog)	KSEQSGAK_______	K209
HSP27 (cow)	KSEQPENK_______	K201

K205-ub

ksDEtAAk_______

HSP27 (human)	ksDEtAAk_______	K205-ub
HSP27 (mouse)	ksEQsGAK_______	K209
HSP27 (rat)	ESEQSGAK_______	K206
HSP27 (pig)	KSEKPGTK_______	K207
HSP27 (hamster)	KSEQSGAK_______	K213
HSP27 (chicken)	KKEEPAKK_______	K193
HSP27 (dog)	KSEQSGAK_______	K209
HSP27 (cow)	KSEQPENK_______	K201