ER-alpha (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

T7-p

_MTMTLHtKAsGMAL

ER-alpha (human)	_MTMTLHtKAsGMAL	T7-p
ER-alpha (mouse)	_MTMTLHTKAsGMAL	T7
ER-alpha (rat)	_MTMTLHTKASGMAL	T7

S10-p

MTLHtKAsGMALLHQ

ER-alpha (human)	MTLHtKAsGMALLHQ	S10-p
ER-alpha (mouse)	MTLHTKASGMALLHQ	S10
ER-alpha (rat)	MTLHTKASGMALLHQ	S10

S10

MTLHtKASGMALLHQ

ER-alpha (human)	MTLHtKASGMALLHQ	S10
ER-alpha (mouse)	MTLHTKAsGMALLHQ	S10-gl
ER-alpha (rat)	MTLHTKASGMALLHQ	S10

K32-ac

PLNRPQLkIPLERPL

ER-alpha (human)	PLNRPQLkIPLERPL	K32-ac
ER-alpha (mouse)	PLNRPQLKMPMERAL	K32
ER-alpha (rat)	PLNRPQLKMPMERAL	K32

S46-p

LGEVYLDssKPAVyN

Downstream

Effects on Modified Protein

activity, induced

Effects on Biological Processes:

transcription, altered

ER-alpha (human)	LGEVYLDssKPAVyN	S46-p
ER-alpha (mouse)	LGEVYVDNSKPtVFN	N46
ER-alpha (rat)	LGEVYVDNSKPAVFN	N46

S47-p

GEVYLDssKPAVyNY

Downstream

Effects on Modified Protein

activity, induced

Effects on Biological Processes:

transcription, altered

ER-alpha (human)	GEVYLDssKPAVyNY	S47-p
ER-alpha (mouse)	GEVYVDNSKPtVFNY	S47
ER-alpha (rat)	GEVYVDNSKPAVFNY	S47

A50

YLDssKPAVyNYPEG

ER-alpha (human)	YLDssKPAVyNYPEG	A50
ER-alpha (mouse)	YVDNSKPtVFNYPEG	T50-gl
ER-alpha (rat)	YVDNSKPAVFNYPEG	A50

Y52-p

DssKPAVyNYPEGAA

Upstream

Downstream

Effects on Modified Protein

protein stabilization

Effects on Biological Processes:

carcinogenesis, induced
cell growth, induced
transcription, induced

Kinase, in vitro:

Abl (human)

Putative in vivo kinases:

Abl (human)

Treatment

imatinib

ER-alpha (human)	DssKPAVyNYPEGAA	Y52-p
ER-alpha (mouse)	DNSKPtVFNYPEGAA	F52
ER-alpha (rat)	DNSKPAVFNYPEGAA	F52

S102-p

GGFPPLNsVsPsPLM

Upstream

Downstream

Effects on Modified Protein

activity, induced

Effects on Biological Processes:

transcription, induced

Kinase, in vitro:

GSK3B (human)

Putative in vivo kinases:

GSK3B (human)

Treatment

estradiol
lithium

ER-alpha (human)	GGFPPLNsVsPsPLM	S102-p
ER-alpha (mouse)	GAFPQLNSVSPSPLM	S106
ER-alpha (rat)	GAFPQLNSVSPSPLM	S107

S104-p

FPPLNsVsPsPLMLL

Upstream

Downstream

Effects on Modified Protein

activity, induced
molecular association, regulation

Effects on Biological Processes:

carcinogenesis, induced
cell growth, induced
cell motility, induced
transcription, altered
transcription, induced

Kinase, in vitro:

CDK2 (human)
CDK3 (human)
ERK2 (human)
GSK3B (human)
mTOR (human)

Putative in vivo kinases:

CDK3 (human)
ERK1 (human)
ERK2 (human)
GSK3B (human)
mTOR (human)

Regulatory protein:

HER2 (human)
HRas (human)
IGF1R (human)
MEMO1 (human)
MLL3 (human)
RAF1 (human)
Raptor (mouse)

Treatment

17-beta-estradiol
4-HT
cholera_toxin
EGF
estradiol
faslodex
GDNF
IBMX
ICI164,384
lithium
LY294002
PD98059
phorbol_ester
siRNA
TOT
U0126

ER-alpha (human)	FPPLNsVsPsPLMLL	S104-p
ER-alpha (mouse)	FPQLNSVSPSPLMLL	S108
ER-alpha (rat)	FPQLNSVSPSPLMLL	S109

S106-p

PLNsVsPsPLMLLHP

Upstream

Downstream

Effects on Modified Protein

activity, induced
molecular association, regulation

Effects on Biological Processes:

carcinogenesis, induced
cell growth, induced
cell motility, induced
transcription, altered
transcription, induced

Kinase, in vitro:

CDK2 (human)
CDK3 (human)
ERK2 (human)
GSK3B (human)
mTOR (human)

Putative in vivo kinases:

CDK3 (human)
ERK1 (human)
ERK2 (human)
GSK3B (human)
mTOR (human)

Regulatory protein:

AREG (human)
HER2 (human)
HRas (human)
IGF1R (human)
MEMO1 (human)
MLL3 (human)
RAF1 (human)
Raptor (mouse)

Treatment

17-beta-estradiol
4-HT
bisphenol_A
cholera_toxin
EGF
estradiol
faslodex
GDNF
IBMX
ICI164,384
lithium
LY294002
PD98059
phorbol_ester
siRNA
TOT
U0126

ER-alpha (human)	PLNsVsPsPLMLLHP	S106-p
ER-alpha (mouse)	QLNSVSPSPLMLLHP	S110
ER-alpha (rat)	QLNSVSPSPLMLLHP	S111

S118-p

LHPPPQLsPFLQPHG

Upstream

Downstream

ER-alpha (human)
S118-p

2511 : Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb

ER-alpha (mouse)
S122-p

2511 : Phospho-Estrogen Receptor alpha (Ser118) (16J4) Mouse mAb

Effects on Modified Protein

acetylation
activity, induced
molecular association, regulation
phosphorylation
protein degradation

Effects on Biological Processes:

carcinogenesis, altered
carcinogenesis, induced
cell growth, altered
cell growth, induced
signaling pathway regulation
transcription, altered
transcription, induced

Kinase, in vitro:

CDK2 (human)
CDK3 (human)
CDK7 (human)
ERK1 (human)
ERK2 (human)
GSK3B (human)
IKKA (human)

Phosphatases, in vitro:

PPP2CB (human)

Putative in vivo kinases:

CDK3 (human)
CDK7 (human)
ERK1 (human)
ERK2 (human)
GSK3B (human)
P38A (human)

Putative upstream phosphatases:

PPP2CA (human)
PPP2CB (human)
PPP5C (human)

Regulatory protein:

Akt1 (human)
DUSP22 (human)
DUSP6 (human)
EGFR (human)
ER-alpha (human)
FAS (human)
FKBPL (human)
HER2 (human)
HRas (human)
IGF1R (human)
ILK (human)
Lmr3 (human)
MEK1 (human)
MEMO1 (human)
MKK6 (human)
MLL3 (human)
NR2F1 (human)
P38A (human)
PAK1 (human)
RAF1 (human)
Ret (human)
TGFBR2 (human)
XPG (human)

Treatment

17-beta-estradiol
3-aminobenzamide
4-HT
AG490
Akt_inhibitor_VIII
AMP-PNP
ATP
Bay_11-7082
black_tea_polyphenols
catalase
cholera_toxin
colforsin
DRB
EGF
estradiol
ethanol
faslodex
GDNF
geldanamycin
genistein
glucose_oxidase
IBMX
ICI164,384
ICI_164384
IGF-1
insulin
KU60648
levothyroxine sodium
lithium
LY294002
Norcantharidin
NU7441
okadaic_acid
PD184352
PD98059
pertuzumab
phorbol_ester
PP2
PRL
QLT0267
raloxifene
rapamycin
SB203580
seliciclib
SERM
serum
siRNA
SP600125
SU6656
tamoxifen
tectoridin
TGF-beta
THZ1
TOT
U0126
wortmannin

ER-alpha (human)	LHPPPQLsPFLQPHG	S118-p
ER-alpha (mouse)	LHPPPQLsPFLHPHG	S122-p
ER-alpha (rat)	LHPPPHVsPFLHPHG	S123-p

P152

GPPAFYRPNsDNRRQ

ER-alpha (human)	GPPAFYRPNsDNRRQ	P152
ER-alpha (mouse)	GPPAFYRsNsDNRRQ	S156-p
ER-alpha (rat)	GPPAFYRSNSDNRRQ	S157

S154-p

PAFYRPNsDNRRQGG

Upstream

Treatment

EGF
estradiol

ER-alpha (human)	PAFYRPNsDNRRQGG	S154-p
ER-alpha (mouse)	PAFYRsNsDNRRQNG	S158-p
ER-alpha (rat)	PAFYRSNSDNRRQNG	S159

S167-p

GGRERLAsTNDkGSM

Upstream

Downstream

ER-alpha (human)
S167-p

Effects on Modified Protein

acetylation
activity, induced
molecular association, regulation
phosphorylation

Effects on Biological Processes:

apoptosis, inhibited
cell growth, altered
signaling pathway regulation
transcription, altered
transcription, induced

Kinase, in vitro:

Akt1 (human)
AurA (human)
CK2A1 (human)
IKKE (human)
p70S6K (human)
p90RSK (human)

Putative in vivo kinases:

Akt1 (human)
Akt2 (human)
AurA (human)
IKKE (human)
p70S6K (human)
p90RSK (human)
RSK2 (human)

Regulatory protein:

Akt1 (human)
AREG (human)
EGFR (human)
HER2 (human)
IGF1R (human)
Lmr3 (human)
MEMO1 (human)
p70S6K (human)
p90RSK (human)
Ret (human)
RSK2 (human)

Treatment

17-beta-estradiol
2-deoxyglucose
3-aminobenzamide
4-HT
bisphenol_A
BX795
catalase
EGF
estradiol
faslodex
GDNF
glucose_oxidase
heregulin
IGF-1
insulin
ischemia
LY294002
Norcantharidin
PD98059
phorbol_ester
rapamycin
SB203580
siRNA
SP600125
SU6656
U0126
wortmannin

ER-alpha (human)	GGRERLAsTNDkGSM	S167-p
ER-alpha (mouse)	NGRERLSsSNEKGNM	S171-p
ER-alpha (rat)	NGRERLSSSSEKGNM	S172

K171-sm

RLAsTNDkGSMAMES

ER-alpha (human)	RLAsTNDkGSMAMES	K171-sm
ER-alpha (mouse)	RLSsSNEKGNMIMES	K175
ER-alpha (rat)	RLSSSSEKGNMIMES	K176

K171-ac

RLAsTNDkGSMAMES

ER-alpha (human)	RLAsTNDkGSMAMES	K171-ac
ER-alpha (mouse)	RLSsSNEKGNMIMES	K175
ER-alpha (rat)	RLSSSSEKGNMIMES	K176

K171-m2

RLAsTNDkGSMAMES

ER-alpha (human)	RLAsTNDkGSMAMES	K171-m2
ER-alpha (mouse)	RLSsSNEKGNMIMES	K175
ER-alpha (rat)	RLSSSSEKGNMIMES	K176

K171-m1

RLAsTNDkGSMAMES

ER-alpha (human)	RLAsTNDkGSMAMES	K171-m1
ER-alpha (mouse)	RLSsSNEKGNMIMES	K175
ER-alpha (rat)	RLSSSSEKGNMIMES	K176

K180-sm

SMAMESAkETRYCAV

ER-alpha (human)	SMAMESAkETRYCAV	K180-sm
ER-alpha (mouse)	NMIMESAKETRYCAV	K184
ER-alpha (rat)	NMIMESAKETRYCAV	K185

K180-m2

SMAMESAkETRYCAV

ER-alpha (human)	SMAMESAkETRYCAV	K180-m2
ER-alpha (mouse)	NMIMESAKETRYCAV	K184
ER-alpha (rat)	NMIMESAKETRYCAV	K185

S212-p

CKAFFKRsIQGHNDy

Upstream

Downstream

Effects on Biological Processes:

transcription, induced

Treatment

estradiol

ER-alpha (human)	CKAFFKRsIQGHNDy	S212-p
ER-alpha (mouse)	CKAFFKRsIQGHNDY	S216-p
ER-alpha (rat)	CKAFFKRSIQGHNDY	S217

Y219-p

sIQGHNDyMCPATNQ

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein stabilization

Effects on Biological Processes:

carcinogenesis, induced
cell growth, induced
signaling pathway regulation
transcription, induced

Kinase, in vitro:

Abl (human)
IGF1R (human)

Putative in vivo kinases:

Abl (human)
IGF1R (human)

Regulatory protein:

PRMT1 (human)

Treatment

imatinib

ER-alpha (human)	sIQGHNDyMCPATNQ	Y219-p
ER-alpha (mouse)	sIQGHNDYMCPATNQ	Y223
ER-alpha (rat)	SIQGHNDYMCPATNQ	Y224

S236-p

IDKNRRKsCQACRLR

Upstream

Downstream

Effects on Biological Processes:

transcription, inhibited

Kinase, in vitro:

PKACA (human)

Putative in vivo kinases:

PKACA (human)

Treatment

cAMP_analog
colforsin
estradiol
IBMX

ER-alpha (human)	IDKNRRKsCQACRLR	S236-p
ER-alpha (mouse)	IDKNRRKSCQACRLR	S240
ER-alpha (rat)	IDKNRRKSCQACRLR	S241

K244-ac

CQACRLRkCyEVGMM

ER-alpha (human)	CQACRLRkCyEVGMM	K244-ac
ER-alpha (mouse)	CQACRLRKCYEVGMM	K248
ER-alpha (rat)	CQACRLRKCyEVGMM	K249

Y246-p

ACRLRkCyEVGMMkG

ER-alpha (human)	ACRLRkCyEVGMMkG	Y246-p
ER-alpha (mouse)	ACRLRKCYEVGMMKG	Y250
ER-alpha (rat)	ACRLRKCyEVGMMKG	Y251-p

K252-ac

CyEVGMMkGGIRKDR

ER-alpha (human)	CyEVGMMkGGIRKDR	K252-ac
ER-alpha (mouse)	CYEVGMMKGGIRKDR	K256
ER-alpha (rat)	CyEVGMMKGGIRKDR	K257

R260-m2

GGIRKDRrGGRMLkH

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

cell cycle regulation

Treatment

17-beta-estradiol

ER-alpha (human)	GGIRKDRrGGRMLkH	R260-m2
ER-alpha (mouse)	GGIRKDRRGGRMLKH	R264
ER-alpha (rat)	GGIRKDRRGGRMLKH	R265

K266-ac

RrGGRMLkHkRQRDD

Upstream

Downstream

Effects on Modified Protein

activity, induced
molecular association, regulation

Effects on Biological Processes:

transcription, altered

Regulatory protein:

BRCA1 (human)

Treatment

estradiol

ER-alpha (human)	RrGGRMLkHkRQRDD	K266-ac
ER-alpha (mouse)	RRGGRMLKHKRQRDD	K270
ER-alpha (rat)	RRGGRMLKHKRQRDD	K271

K266-sm

RrGGRMLkHkRQRDD

ER-alpha (human)	RrGGRMLkHkRQRDD	K266-sm
ER-alpha (mouse)	RRGGRMLKHKRQRDD	K270
ER-alpha (rat)	RRGGRMLKHKRQRDD	K271

K266-m1

RrGGRMLkHkRQRDD

ER-alpha (human)	RrGGRMLkHkRQRDD	K266-m1
ER-alpha (mouse)	RRGGRMLKHKRQRDD	K270
ER-alpha (rat)	RRGGRMLKHKRQRDD	K271

K268-ac

GGRMLkHkRQRDDGE

Upstream

Downstream

Effects on Modified Protein

activity, induced
molecular association, regulation

Effects on Biological Processes:

transcription, altered

Regulatory protein:

BRCA1 (human)

Treatment

estradiol

ER-alpha (human)	GGRMLkHkRQRDDGE	K268-ac
ER-alpha (mouse)	GGRMLKHKRQRDDLE	K272
ER-alpha (rat)	GGRMLKHKRQRDDLE	K273

K268-sm

GGRMLkHkRQRDDGE

ER-alpha (human)	GGRMLkHkRQRDDGE	K268-sm
ER-alpha (mouse)	GGRMLKHKRQRDDLE	K272
ER-alpha (rat)	GGRMLKHKRQRDDLE	K273

R277-m2

QRDDGEGrGEVGsAG

ER-alpha (human)	QRDDGEGrGEVGsAG	R277-m2
ER-alpha (mouse)	QRDDLEGRNEMGAsG	R281
ER-alpha (rat)	QRDDLEGRNEMGTSG	R282

S282-p

EGrGEVGsAGDMRAA

Upstream

Downstream

Effects on Modified Protein

activity, inhibited

Effects on Biological Processes:

transcription, altered

Kinase, in vitro:

CK2A1 (human)

Putative in vivo kinases:

CK2A1 (human)

Treatment

DMAT
estradiol

ER-alpha (human)	EGrGEVGsAGDMRAA	S282-p
ER-alpha (mouse)	EGRNEMGAsGDMRAA	A286
ER-alpha (rat)	EGRNEMGTSGDMRAA	T287

A283

GrGEVGsAGDMRAAN

ER-alpha (human)	GrGEVGsAGDMRAAN	A283
ER-alpha (mouse)	GRNEMGAsGDMRAAN	S287-p
ER-alpha (rat)	GRNEMGTSGDMRAAN	S288

S294-p

RAANLWPsPLMIkRS

Upstream

Downstream

Effects on Modified Protein

activity, induced
molecular association, regulation
protein conformation
protein degradation
ubiquitination

Effects on Biological Processes:

cell cycle regulation
transcription, altered
transcription, induced

Kinase, in vitro:

CDK2 (human)
P38A (human)

Putative in vivo kinases:

CDK2 (human)
P38A (human)

Regulatory protein:

SKP2 (human)

Treatment

17-beta-estradiol
dinaciclib
estradiol
MG132
palbociclib
SB203580

ER-alpha (human)	RAANLWPsPLMIkRS	S294-p
ER-alpha (mouse)	RAANLWPsPLVIKHT	S298-p
ER-alpha (rat)	RAANLWPSPLVIKHT	S299

K299-ac

WPsPLMIkRSkkNsL

ER-alpha (human)	WPsPLMIkRSkkNsL	K299-ac
ER-alpha (mouse)	WPsPLVIKHTKKNSP	K303
ER-alpha (rat)	WPSPLVIKHTKKNSP	K304

K299-sm

WPsPLMIkRSkkNsL

ER-alpha (human)	WPsPLMIkRSkkNsL	K299-sm
ER-alpha (mouse)	WPsPLVIKHTKKNSP	K303
ER-alpha (rat)	WPSPLVIKHTKKNSP	K304

K302-ac

PLMIkRSkkNsLALS

Upstream

Downstream

Effects on Modified Protein

activity, induced

Effects on Biological Processes:

transcription, altered

Regulatory protein:

BRCA1 (human)

ER-alpha (human)	PLMIkRSkkNsLALS	K302-ac
ER-alpha (mouse)	PLVIKHTKKNSPALS	K306
ER-alpha (rat)	PLVIKHTKKNSPALS	K307

K302-ub

PLMIkRSkkNsLALS

Downstream

Effects on Modified Protein

activity, inhibited

Effects on Biological Processes:

transcription, altered

ER-alpha (human)	PLMIkRSkkNsLALS	K302-ub
ER-alpha (mouse)	PLVIKHTKKNSPALS	K306
ER-alpha (rat)	PLVIKHTKKNSPALS	K307

K302-m1

PLMIkRSkkNsLALS

ER-alpha (human)	PLMIkRSkkNsLALS	K302-m1
ER-alpha (mouse)	PLVIKHTKKNSPALS	K306
ER-alpha (rat)	PLVIKHTKKNSPALS	K307

K302-me

PLMIkRSkkNsLALS

ER-alpha (human)	PLMIkRSkkNsLALS	K302-me
ER-alpha (mouse)	PLVIKHTKKNSPALS	K306
ER-alpha (rat)	PLVIKHTKKNSPALS	K307

K303-ac

LMIkRSkkNsLALSL

Upstream

Downstream

Effects on Modified Protein

activity, induced
activity, inhibited

Effects on Biological Processes:

transcription, altered

Regulatory protein:

BRCA1 (human)

ER-alpha (human)	LMIkRSkkNsLALSL	K303-ac
ER-alpha (mouse)	LVIKHTKKNSPALSL	K307
ER-alpha (rat)	LVIKHTKKNSPALSL	K308

S305-p

IkRSkkNsLALSLtA

Upstream

Downstream

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
phosphorylation
protein conformation
protein stabilization

Effects on Biological Processes:

carcinogenesis, altered
carcinogenesis, induced
cell growth, altered
cell growth, induced
transcription, altered
transcription, induced

Kinase, in vitro:

Akt1 (human)
AurA (human)
PAK1 (human)
PAK4 (human)
PKACA (human)

Putative in vivo kinases:

AurA (human)
PAK1 (human)
PKACA (human)
TBK1 (human)

Regulatory protein:

AKAP13 (human)
IKKE (human)
PAK1 (human)
PKAR1A (human)

Treatment

BX795
cAMP_analog
colforsin
estradiol
heregulin
peptide inhibitor
siRNA
tamoxifen

ER-alpha (human)	IkRSkkNsLALSLtA	S305-p
ER-alpha (mouse)	IKHTKKNSPALSLTA	S309
ER-alpha (rat)	IKHTKKNSPALSLTA	S310

T311-p

NsLALSLtADQMVSA

Upstream

Downstream

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation

Effects on Biological Processes:

transcription, altered

Kinase, in vitro:

P38A (human)

Putative in vivo kinases:

P38A (human)

Treatment

Auranofin
CysNO
estradiol
SB203580

ER-alpha (human)	NsLALSLtADQMVSA	T311-p
ER-alpha (mouse)	NSPALSLTADQMVSA	T315
ER-alpha (rat)	NSPALSLTADQMVSA	T316

S341-p

TRPFSEAsMMGLLTN

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein degradation
ubiquitination

Effects on Biological Processes:

cell cycle regulation
transcription, induced

Kinase, in vitro:

CDK2 (human)

Treatment

estradiol

ER-alpha (human)	TRPFSEAsMMGLLTN	S341-p
ER-alpha (mouse)	SRPFSEASMMGLLTN	S345
ER-alpha (rat)	SRPFSEASMMGLLTN	S346

K401-m2

RSMEHPGkLLFAPNL

ER-alpha (human)	RSMEHPGkLLFAPNL	K401-m2
ER-alpha (mouse)	RSMEHPGKLLFAPNL	K405
ER-alpha (rat)	RSMEHPGKLLFAPNL	K406

S450

EEFVCLKSIILLNSG

ER-alpha (human)	EEFVCLKSIILLNSG	S450
ER-alpha (mouse)	EEFVCLKSIILLNSG	S454
ER-alpha (rat)	EEFVCLKsIILLNSG	S455-p

T460

LLNSGVYTFLSSTLK

ER-alpha (human)	LLNSGVYTFLSSTLK	T460
ER-alpha (mouse)	LLNSGVYtFLSsTLK	T464-p
ER-alpha (rat)	LLNSGVYTFLSsTLK	T465

S464

GVYTFLSSTLKSLEE

ER-alpha (human)	GVYTFLSSTLKSLEE	S464
ER-alpha (mouse)	GVYtFLSsTLKSLEE	S468-p
ER-alpha (rat)	GVYTFLSsTLKSLEE	S469-p

K472-ac

TLKSLEEkDHIHRVL

Upstream

Treatment

estradiol

ER-alpha (human)	TLKSLEEkDHIHRVL	K472-ac
ER-alpha (mouse)	TLKSLEEKDHIHRVL	K476
ER-alpha (rat)	TLKSLEEKDHIHRVL	K477

K472-sm

TLKSLEEkDHIHRVL

ER-alpha (human)	TLKSLEEkDHIHRVL	K472-sm
ER-alpha (mouse)	TLKSLEEKDHIHRVL	K476
ER-alpha (rat)	TLKSLEEKDHIHRVL	K477

T485-p

VLDKITDtLIHLMAK

ER-alpha (human)	VLDKITDtLIHLMAK	T485-p
ER-alpha (mouse)	VLDKITDTLIHLMAK	T489
ER-alpha (rat)	VLDKINDTLIHLMAK	T490

Y537-p

CKNVVPLyDLLLEML

Upstream

Downstream

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
protein conformation
protein stabilization

Effects on Biological Processes:

cell growth, induced
transcription, induced

Kinase, in vitro:

Lck (human)
Src (human)

Phosphatases, in vitro:

PTP1B (human)
SHP-1 (human)

Treatment

colforsin
estradiol
faslodex
IBMX
tamoxifen

ER-alpha (human)	CKNVVPLyDLLLEML	Y537-p
ER-alpha (mouse)	CKNVVPLYDLLLEML	Y541
ER-alpha (rat)	CKNVVPLYDLLLEML	Y542

S554-p

HRLHAPTsRGGAsVE

Upstream

Treatment

estradiol

ER-alpha (human)	HRLHAPTsRGGAsVE	S554-p
ER-alpha (mouse)	HRLHAPASRMGVPPE	S558
ER-alpha (rat)	HRLHAPASRMGVPPE	S559

S559-p

PTsRGGAsVEETDQS

Upstream

Downstream

Effects on Modified Protein

activity, inhibited

Effects on Biological Processes:

transcription, altered

Kinase, in vitro:

CK2A1 (human)

Putative in vivo kinases:

CK2A1 (human)

Treatment

DMAT
estradiol

ER-alpha (human)	PTsRGGAsVEETDQS	S559-p
ER-alpha (mouse)	PASRMGVPPEEPSQT	P563
ER-alpha (rat)	PASRMGVPPEEPSQS	P564

A571

DQSHLATAGSTSsHs

ER-alpha (human)	DQSHLATAGSTSsHs	A571
ER-alpha (mouse)	SQTQLATtSSTSAHS	T575-gl
ER-alpha (rat)	SQSQLTTTSSTSAHS	T576

S576-p

ATAGSTSsHsLQKYY

ER-alpha (human)	ATAGSTSsHsLQKYY	S576-p
ER-alpha (mouse)	ATtSSTSAHSLQTYY	A580
ER-alpha (rat)	TTTSSTSAHSLQTYY	A581

S578-p

AGSTSsHsLQKYYIT

ER-alpha (human)	AGSTSsHsLQKYYIT	S578-p
ER-alpha (mouse)	tSSTSAHSLQTYYIP	S582
ER-alpha (rat)	TSSTSAHSLQTYYIP	S583

T594-p

EAEGFPAtV______

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

cell growth, altered
transcription, altered

Treatment

fusicoccin
MG132
phosphorylated peptide

ER-alpha (human)	EAEGFPAtV______	T594-p
ER-alpha (mouse)	EAEGFPNTI______	T598
ER-alpha (rat)	EAEGFPNTI______	T599