RAF1 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

S12-p

QGAWKTIsNGFGFkD

RAF1 (human)	QGAWKTIsNGFGFkD	S12-p
RAF1 iso2 (human)	QGAWKTISNGFGFKD	S12
RAF1 (mouse)	QGAWKTISNGFGLKD	S12
RAF1 (rat)	QGAWKTISNGFGLKD	S12
RAF1 (chicken)	QGAWKTISNGFGLKD	S12

K18-m1

IsNGFGFkDAVFDGS

RAF1 (human)	IsNGFGFkDAVFDGS	K18-m1
RAF1 iso2 (human)	ISNGFGFKDAVFDGS	K18
RAF1 (mouse)	ISNGFGLKDAVFDGS	K18
RAF1 (rat)	ISNGFGLKDAVFDGS	K18
RAF1 (chicken)	ISNGFGLKDSVFDGP	K18

S26

DAVFDGSSCIsPtIV

RAF1 (human)	DAVFDGSSCIsPtIV	S26
RAF1 iso2 (human)	DAVFDGSSCISPTIV	S26
RAF1 (mouse)	DAVFDGSsCIsPtIV	S26-p
RAF1 (rat)	DAVFDGSSCISPTIV	S26
RAF1 (chicken)	DSVFDGPNCISPTIV	N26

S29-p

FDGSSCIsPtIVQQF

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation

Treatment

ischemia
okadaic_acid
PDGF
U0126

RAF1 (human)	FDGSSCIsPtIVQQF	S29-p
RAF1 iso2 (human)	FDGSSCISPTIVQQF	S29
RAF1 (mouse)	FDGSsCIsPtIVQQF	S29-p
RAF1 (rat)	FDGSSCISPTIVQQF	S29
RAF1 (chicken)	FDGPNCISPTIVQQF	S29

T31-p

GSSCIsPtIVQQFGy

RAF1 (human)	GSSCIsPtIVQQFGy	T31-p
RAF1 iso2 (human)	GSSCISPTIVQQFGY	T31
RAF1 (mouse)	GSsCIsPtIVQQFGY	T31-p
RAF1 (rat)	GSSCISPTIVQQFGY	T31
RAF1 (chicken)	GPNCISPTIVQQFGY	T31

Y38-p

tIVQQFGyQRRAsDD

RAF1 (human)	tIVQQFGyQRRAsDD	Y38-p
RAF1 iso2 (human)	TIVQQFGYQRRASDD	Y38
RAF1 (mouse)	tIVQQFGYQRRAsDD	Y38
RAF1 (rat)	TIVQQFGYQRRAsDD	Y38
RAF1 (chicken)	TIVQQFGYQRRASDD	Y38

S43-p

FGyQRRAsDDGKLtD

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation

Kinase, in vitro:

PKACA (cow)
PKACA (human)
PKCA (human)

Putative in vivo kinases:

PKACA (mouse)

Treatment

colforsin
EGF
IBMX
ischemia
M-CSF
okadaic_acid
PDGF
phorbol_ester
U0126

RAF1 (human)	FGyQRRAsDDGKLtD	S43-p
RAF1 iso2 (human)	FGYQRRASDDGKLTD	S43
RAF1 (mouse)	FGYQRRAsDDGKLTD	S43-p
RAF1 (rat)	FGYQRRAsDDGKLTD	S43-p
RAF1 (chicken)	FGYQRRASDDGKISD	S43

T49-p

AsDDGKLtDPSkTSN

RAF1 (human)	AsDDGKLtDPSkTSN	T49-p
RAF1 iso2 (human)	ASDDGKLTDPSKTSN	T49
RAF1 (mouse)	AsDDGKLTDSSKTSN	T49
RAF1 (rat)	AsDDGKLTDSSKTSN	T49
RAF1 (chicken)	ASDDGKISDTSKTSN	S49

K53-ub

GKLtDPSkTSNTIRV

RAF1 (human)	GKLtDPSkTSNTIRV	K53-ub
RAF1 iso2 (human)	GKLTDPSKTSNTIRV	K53
RAF1 (mouse)	GKLTDSSKTSNtIRV	K53
RAF1 (rat)	GKLTDSSKTSNTIRV	K53
RAF1 (chicken)	GKISDTSKTSNTIRV	K53

T57

DPSkTSNTIRVFLPN

RAF1 (human)	DPSkTSNTIRVFLPN	T57
RAF1 iso2 (human)	DPSKTSNTIRVFLPN	T57
RAF1 (mouse)	DSSKTSNtIRVFLPN	T57-p
RAF1 (rat)	DSSKTSNTIRVFLPN	T57
RAF1 (chicken)	DTSKTSNTIRVFLPN	T57

K65-ub

IRVFLPNkQRTVVNV

RAF1 (human)	IRVFLPNkQRTVVNV	K65-ub
RAF1 iso2 (human)	IRVFLPNKQRTVVNV	K65
RAF1 (mouse)	IRVFLPNkQRTVVNV	K65-ub
RAF1 (rat)	IRVFLPNKQRTVVNV	K65
RAF1 (chicken)	IRVFLPNKQRTVVNV	K65

R100-me

PECCAVFrLLHEHKG

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

carcinogenesis, inhibited
cell motility, inhibited
signaling pathway regulation

Treatment

siRNA

RAF1 (human)	PECCAVFrLLHEHKG	R100-me
RAF1 iso2 (human)	PECCAVFRLLHEHKG	R100
RAF1 (mouse)	PECCAVFRLLQEHKG	R100
RAF1 (rat)	PECCAVFRLLQEHKG	R100
RAF1 (chicken)	PECCAVFRLVTEPKG	R100

S211-p

SGVPALPsLtMRRMR

RAF1 (human)	SGVPALPsLtMRRMR	S211-p
RAF1 iso2 (human)	SGVPALPSLTMRRMR	S211
RAF1 (mouse)	SGVPAPPSFPMRRMR	S211
RAF1 (rat)	SGVPAPPSFTMRRMR	S211
RAF1 (chicken)	SGVPALPPLTMRRMR	P211

T213-p

VPALPsLtMRRMREs

RAF1 (human)	VPALPsLtMRRMREs	T213-p
RAF1 iso2 (human)	VPALPSLTMRRMRES	T213
RAF1 (mouse)	VPAPPSFPMRRMREs	P213
RAF1 (rat)	VPAPPSFTMRRMRES	T213
RAF1 (chicken)	VPALPPLTMRRMRES	T213

S220-p

tMRRMREsVSrMPVS

Upstream

Treatment

ischemia

RAF1 (human)	tMRRMREsVSrMPVS	S220-p
RAF1 iso2 (human)	TMRRMRESVSRMPVS	S220
RAF1 (mouse)	PMRRMREsVSRMPAs	S220-p
RAF1 (rat)	TMRRMRESVSRMPAS	S220
RAF1 (chicken)	TMRRMRESVSRIPVS	S220

R223-m1

RMREsVSrMPVSSQH

RAF1 (human)	RMREsVSrMPVSSQH	R223-m1
RAF1 iso2 (human)	RMRESVSRMPVSSQH	R223
RAF1 (mouse)	RMREsVSRMPAsSQH	R223
RAF1 (rat)	RMRESVSRMPASSQH	R223
RAF1 (chicken)	RMRESVSRIPVSSQH	R223

S227

sVSrMPVSSQHryst

RAF1 (human)	sVSrMPVSSQHryst	S227
RAF1 iso2 (human)	SVSRMPVSSQHRYST	S227
RAF1 (mouse)	sVSRMPAsSQHRYst	S227-p
RAF1 (rat)	SVSRMPASSQHRYST	S227
RAF1 (chicken)	SVSRIPVSSQHRYST	S227

R231-m1

MPVSSQHrystPHAF

RAF1 (human)	MPVSSQHrystPHAF	R231-m1
RAF1 iso2 (human)	MPVSSQHRYSTPHAF	R231
RAF1 (mouse)	MPAsSQHRYstPHAF	R231
RAF1 (rat)	MPASSQHRYSTPHAF	R231
RAF1 (chicken)	IPVSSQHRYSTPHVF	R231

Y232-p

PVSSQHrystPHAFt

RAF1 (human)	PVSSQHrystPHAFt	Y232-p
RAF1 iso2 (human)	PVSSQHRYSTPHAFT	Y232
RAF1 (mouse)	PAsSQHRYstPHAFT	Y232
RAF1 (rat)	PASSQHRYSTPHAFT	Y232
RAF1 (chicken)	PVSSQHRYSTPHVFT	Y232

S233-p

VSSQHrystPHAFtF

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation

Regulatory protein:

CD38 (human)

Treatment

colforsin
EGF
IBMX

RAF1 (human)	VSSQHrystPHAFtF	S233-p
RAF1 iso2 (human)	VSSQHRYSTPHAFTF	S233
RAF1 (mouse)	AsSQHRYstPHAFTF	S233-p
RAF1 (rat)	ASSQHRYSTPHAFTF	S233
RAF1 (chicken)	VSSQHRYSTPHVFTF	S233

T234-p

SSQHrystPHAFtFN

RAF1 (human)	SSQHrystPHAFtFN	T234-p
RAF1 iso2 (human)	SSQHRYSTPHAFTFN	T234
RAF1 (mouse)	sSQHRYstPHAFTFN	T234-p
RAF1 (rat)	SSQHRYSTPHAFTFN	T234
RAF1 (chicken)	SSQHRYSTPHVFTFN	T234

T239-p

ystPHAFtFNtSsPS

RAF1 (human)	ystPHAFtFNtSsPS	T239-p
RAF1 iso2 (human)	YSTPHAFTFNTSSPS	T239
RAF1 (mouse)	YstPHAFTFNtSsPS	T239
RAF1 (rat)	YSTPHAFTFNTSSPS	T239
RAF1 (chicken)	YSTPHVFTFNTSNPS	T239

T242-p

PHAFtFNtSsPSsEG

RAF1 (human)	PHAFtFNtSsPSsEG	T242-p
RAF1 iso2 (human)	PHAFTFNTSSPSSEG	T242
RAF1 (mouse)	PHAFTFNtSsPSSEG	T242-p
RAF1 (rat)	PHAFTFNTSSPSSEG	T242
RAF1 (chicken)	PHVFTFNTSNPSSEG	T242

S244-p

AFtFNtSsPSsEGsL

Upstream

Treatment

nocodazole
thymidine

RAF1 (human)	AFtFNtSsPSsEGsL	S244-p
RAF1 iso2 (human)	AFTFNTSSPSSEGSL	S244
RAF1 (mouse)	AFTFNtSsPSSEGSL	S244-p
RAF1 (rat)	AFTFNTSSPSSEGSL	S244
RAF1 (chicken)	VFTFNTSNPSSEGTL	N244

S247-p

FNtSsPSsEGsLsQR

RAF1 (human)	FNtSsPSsEGsLsQR	S247-p
RAF1 iso2 (human)	FNTSSPSSEGSLSQR	S247
RAF1 (mouse)	FNtSsPSSEGSLSQR	S247
RAF1 (rat)	FNTSSPSSEGSLSQR	S247
RAF1 (chicken)	FNTSNPSSEGTLSQR	S247

S250-p

SsPSsEGsLsQRQRs

RAF1 (human)	SsPSsEGsLsQRQRs	S250-p
RAF1 iso2 (human)	SSPSSEGSLSQRQRS	S250
RAF1 (mouse)	SsPSSEGSLSQRQRS	S250
RAF1 (rat)	SSPSSEGSLSQRQRS	S250
RAF1 (chicken)	SNPSSEGTLSQRQRS	T250

S252-p

PSsEGsLsQRQRsts

RAF1 (human)	PSsEGsLsQRQRsts	S252-p
RAF1 iso2 (human)	PSSEGSLSQRQRSTS	S252
RAF1 (mouse)	PSSEGSLSQRQRSts	S252
RAF1 (rat)	PSSEGSLSQRQRSTs	S252
RAF1 (chicken)	PSSEGTLSQRQRSTS	S252

S257-p

sLsQRQRststPNVH

RAF1 (human)	sLsQRQRststPNVH	S257-p
RAF1 iso2 (human)	SLSQRQRSTSTPNVH	S257
RAF1 (mouse)	SLSQRQRStsTPNVH	S257
RAF1 (rat)	SLSQRQRSTsTPNVH	S257
RAF1 (chicken)	TLSQRQRSTSTPNVH	S257

T258-p

LsQRQRststPNVHM

RAF1 (human)	LsQRQRststPNVHM	T258-p
RAF1 iso2 (human)	LSQRQRSTSTPNVHM	T258
RAF1 (mouse)	LSQRQRStsTPNVHM	T258-p
RAF1 (rat)	LSQRQRSTsTPNVHM	T258
RAF1 (chicken)	LSQRQRSTSTPNVHM	T258

S259-p

sQRQRststPNVHMV

Upstream

Downstream

175

RAF1 (human)
S259-p

RAF1 (mouse)
S259-p

RAF1 (rat)
S259-p

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein stabilization

Effects on Biological Processes:

apoptosis, altered
apoptosis, inhibited
carcinogenesis, induced
carcinogenesis, inhibited
cell cycle regulation
cell differentiation, induced
cell differentiation, inhibited
cell growth, altered
cell growth, induced
cell motility, induced
signaling pathway regulation
transcription, induced

Kinase, in vitro:

AMPKA1 (mouse)
LATS1 (human)
PKACA (human)

Phosphatases, in vitro:

PPP2CA (human)

Putative in vivo kinases:

Akt1 (human)
PKACA (mouse)
RAF1 (human)

Putative upstream phosphatases:

PPP2CB (human)

Regulatory protein:

14-3-3 zeta (human)
Akt1 (human)
ARAF (human)
DDAH1 (human)
ENaC-beta (human)
ERas (human)
LATS1 (human)
LATS2 (human)
LYN (human)
MAD2L1 (human)
MEK1 (human)
MRas (human)
MST2 (human)
osteopontin (human)
PDE8A (human)
RAF1 (human)
SHOC2 (human)

Treatment

5-aza-CdR
Akt-I-1
arachidonic_acid
cAMP_analog
colforsin
curcumin
dronabinol
EAL
EDTA
EGF
GIP
guanidine
H-89
IBMX
IGF-1
lactacystin
low_K(+)
LY294002
M-CSF
okadaic_acid
phorbol_ester
PKC412
PTX
retinoic_acid
rimonabant
seliciclib
serum
SR_144528
taxol
vincristine
virus infection
wortmannin
Y27632
Z-VAD-FMK

RAF1 (human)	sQRQRststPNVHMV	S259-p
RAF1 iso2 (human)	SQRQRSTSTPNVHMV	S259
RAF1 (mouse)	SQRQRStsTPNVHMV	S259-p
RAF1 (rat)	SQRQRSTsTPNVHMV	S259-p
RAF1 (chicken)	SQRQRSTSTPNVHMV	S259

T260-p

QRQRststPNVHMVs

RAF1 (human)	QRQRststPNVHMVs	T260-p
RAF1 iso2 (human)	QRQRSTSTPNVHMVS	T260
RAF1 (mouse)	QRQRStsTPNVHMVS	T260
RAF1 (rat)	QRQRSTsTPNVHMVS	T260
RAF1 (chicken)	QRQRSTSTPNVHMVS	T260

S267-p

tPNVHMVsttLPVDS

RAF1 (human)	tPNVHMVsttLPVDS	S267-p
RAF1 iso2 (human)	TPNVHMVSTTLPVDS	S267
RAF1 (mouse)	TPNVHMVSTTLHVDS	S267
RAF1 (rat)	TPNVHMVSTTLPVDS	S267
RAF1 (chicken)	TPNVHMVSTTMPVDS	S267

T268-p

PNVHMVsttLPVDSR

RAF1 (human)	PNVHMVsttLPVDSR	T268-p
RAF1 iso2 (human)	PNVHMVSTTLPVDSR	T268
RAF1 (mouse)	PNVHMVSTTLHVDSR	T268
RAF1 (rat)	PNVHMVSTTLPVDSR	T268
RAF1 (chicken)	PNVHMVSTTMPVDSR	T268

T269-p

NVHMVsttLPVDSRM

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Kinase, in vitro:

KSR (human)

Putative in vivo kinases:

KSR (human)

Treatment

C8-ceramide
EGF
TNF

RAF1 (human)	NVHMVsttLPVDSRM	T269-p
RAF1 iso2 (human)	NVHMVSTTLPVDSRM	T269
RAF1 (mouse)	NVHMVSTTLHVDSRM	T269
RAF1 (rat)	NVHMVSTTLPVDSRM	T269
RAF1 (chicken)	NVHMVSTTMPVDSRI	T269

S283-p

MIEDAIRsHsEsAsP

RAF1 (human)	MIEDAIRsHsEsAsP	S283-p
RAF1 iso2 (human)	RGRDAIRSHSESASP	S303
RAF1 (mouse)	MIEDAIRSHSESAsP	S283
RAF1 (rat)	MIEDAIRSHSESASP	S283
RAF1 (chicken)	IIEDAIRNHSESASP	N283

S285-p

EDAIRsHsEsAsPsA

RAF1 (human)	EDAIRsHsEsAsPsA	S285-p
RAF1 iso2 (human)	RDAIRSHSESASPSA	S305
RAF1 (mouse)	EDAIRSHSESAsPsA	S285
RAF1 (rat)	EDAIRSHSESASPSA	S285
RAF1 (chicken)	EDAIRNHSESASPSA	S285

S287-p

AIRsHsEsAsPsALs

Downstream

Effects on Modified Protein

activity, induced

Effects on Biological Processes:

cell cycle regulation

RAF1 (human)	AIRsHsEsAsPsALs	S287-p
RAF1 iso2 (human)	AIRSHSESASPSALS	S307
RAF1 (mouse)	AIRSHSESAsPsALs	S287
RAF1 (rat)	AIRSHSESASPSALS	S287
RAF1 (chicken)	AIRNHSESASPSALS	S287

S289-p

RsHsEsAsPsALsss

Upstream

Downstream

RAF1 (human)
S289-p

9431 : Phospho-c-Raf (Ser289/296/301) Antibody

RAF1 (mouse)
S289-p

9431 : Phospho-c-Raf (Ser289/296/301) Antibody

Effects on Modified Protein

activity, induced
enzymatic activity, induced
enzymatic activity, inhibited
intracellular localization
molecular association, regulation

Effects on Biological Processes:

cell cycle regulation
cell differentiation, induced

Kinase, in vitro:

ERK1 (human)

Putative in vivo kinases:

ERK1 (human)

Regulatory protein:

ENaC-beta (human)
ERK1 (human)
LYN (human)

Treatment

EGF
okadaic_acid
PDGF
retinoic_acid
seliciclib
siRNA
U0126

RAF1 (human)	RsHsEsAsPsALsss	S289-p
RAF1 iso2 (human)	RSHSESASPSALSSS	S309
RAF1 (mouse)	RSHSESAsPsALsss	S289-p
RAF1 (rat)	RSHSESASPSALSSs	S289
RAF1 (chicken)	RNHSESASPSALSGS	S289

S291-p

HsEsAsPsALsssPN

Downstream

Effects on Modified Protein

activity, induced

Effects on Biological Processes:

cell cycle regulation

RAF1 (human)	HsEsAsPsALsssPN	S291-p
RAF1 iso2 (human)	HSESASPSALSSSPN	S311
RAF1 (mouse)	HSESAsPsALsssPN	S291-p
RAF1 (rat)	HSESASPSALSSsPN	S291
RAF1 (chicken)	HSESASPSALSGSPN	S291

S294-p

sAsPsALsssPNNLs

RAF1 (human)	sAsPsALsssPNNLs	S294-p
RAF1 iso2 (human)	SASPSALSSSPNNLS	S314
RAF1 (mouse)	SAsPsALsssPNNLs	S294-p
RAF1 (rat)	SASPSALSSsPNNLs	S294
RAF1 (chicken)	SASPSALSGSPNNMS	S294

S295-p

AsPsALsssPNNLsP

RAF1 (human)	AsPsALsssPNNLsP	S295-p
RAF1 iso2 (human)	ASPSALSSSPNNLSP	S315
RAF1 (mouse)	AsPsALsssPNNLsP	S295-p
RAF1 (rat)	ASPSALSSsPNNLsP	S295
RAF1 (chicken)	ASPSALSGSPNNMSP	G295

S296-p

sPsALsssPNNLsPt

Upstream

Downstream

RAF1 (human)
S296-p

RAF1 (mouse)
S296-p

RAF1 (rat)
S296-p

9423 : Phospho-c-Raf (Ser296) Antibody

Effects on Modified Protein

enzymatic activity, induced
enzymatic activity, inhibited
intracellular localization
molecular association, regulation

Effects on Biological Processes:

cell differentiation, induced

Kinase, in vitro:

ERK1 (human)

Putative in vivo kinases:

ERK1 (human)

Regulatory protein:

ERK1 (human)
LYN (human)
TSLP (human)

Treatment

dasatinib
EGF
okadaic_acid
PDGF
retinoic_acid
seliciclib
siRNA
U0126

RAF1 (human)	sPsALsssPNNLsPt	S296-p
RAF1 iso2 (human)	SPSALSSSPNNLSPT	S316
RAF1 (mouse)	sPsALsssPNNLsPt	S296-p
RAF1 (rat)	SPSALSSsPNNLsPT	S296-p
RAF1 (chicken)	SPSALSGSPNNMSPT	S296

S301-p

sssPNNLsPtGWsQP

Upstream

Downstream

RAF1 (human)
S301-p

9431 : Phospho-c-Raf (Ser289/296/301) Antibody

RAF1 (mouse)
S301-p

9431 : Phospho-c-Raf (Ser289/296/301) Antibody

Effects on Modified Protein

enzymatic activity, induced
enzymatic activity, inhibited
intracellular localization
molecular association, regulation

Effects on Biological Processes:

cell differentiation, induced

Kinase, in vitro:

ERK1 (human)

Putative in vivo kinases:

ERK1 (human)

Regulatory protein:

ERK1 (human)
LYN (human)

Treatment

chrysin
EGF
okadaic_acid
PDGF
retinoic_acid
seliciclib
siRNA
U0126

RAF1 (human)	sssPNNLsPtGWsQP	S301-p
RAF1 iso2 (human)	SSSPNNLSPTGWSQP	S321
RAF1 (mouse)	sssPNNLsPtGWsQP	S301-p
RAF1 (rat)	SSsPNNLsPTGWSQP	S301-p
RAF1 (chicken)	SGSPNNMSPTGWSQP	S301

T303-p

sPNNLsPtGWsQPKt

RAF1 (human)	sPNNLsPtGWsQPKt	T303-p
RAF1 iso2 (human)	SPNNLSPTGWSQPKT	T323
RAF1 (mouse)	sPNNLsPtGWsQPKt	T303-p
RAF1 (rat)	sPNNLsPTGWSQPKT	T303
RAF1 (chicken)	SPNNMSPTGWSQPKT	T303

S306-p

NLsPtGWsQPKtPVP

RAF1 (human)	NLsPtGWsQPKtPVP	S306-p
RAF1 iso2 (human)	NLSPTGWSQPKTPVP	S326
RAF1 (mouse)	NLsPtGWsQPKtPVP	S306-p
RAF1 (rat)	NLsPTGWSQPKTPVP	S306
RAF1 (chicken)	NMSPTGWSQPKTPVP	S306

T310-p

tGWsQPKtPVPAQRE

RAF1 (human)	tGWsQPKtPVPAQRE	T310-p
RAF1 iso2 (human)	TGWSQPKTPVPAQRE	T330
RAF1 (mouse)	tGWsQPKtPVPAQRE	T310-p
RAF1 (rat)	TGWSQPKTPVPAQRE	T310
RAF1 (chicken)	TGWSQPKTPVPAQRE	T310

T324-p

ERAPVSGtQEKNKIR

RAF1 (human)	ERAPVSGtQEKNKIR	T324-p
RAF1 iso2 (human)	ERAPVSGTQEKNKIR	T344
RAF1 (mouse)	ERAPGSGTQEKNKIR	T324
RAF1 (rat)	ERAPGSGTQEKNKIR	T324
RAF1 (chicken)	ERAPGTNTQEKNKIR	T324

S338-p

RPRGQRDssyyWEIE

Upstream

Downstream

RAF1 (human)
S338-p

9427 : Phospho-c-Raf (Ser338) (56A6) Rabbit mAb

RAF1 (mouse)
S338-p

9427 : Phospho-c-Raf (Ser338) (56A6) Rabbit mAb

RAF1 (rat)
S338-p

9427 : Phospho-c-Raf (Ser338) (56A6) Rabbit mAb

Effects on Modified Protein

activity, induced
enzymatic activity, induced
intracellular localization
molecular association, regulation
phosphorylation
protein conformation

Effects on Biological Processes:

apoptosis, inhibited
carcinogenesis, induced
cell cycle regulation
cell growth, induced
cell growth, inhibited
cell motility, induced
signaling pathway regulation

Kinase, in vitro:

MEK1 (human)
PAK1 (human)
PAK2 (human)
PAK3 (rat)

Phosphatases, in vitro:

PPP5C (human)

Putative in vivo kinases:

PAK1 (human)
PAK2 (human)
PAK3 (rat)
RAF1 (human)

Putative upstream phosphatases:

PPP5C (human)

Regulatory protein:

CDK10 (human)
DLX3 (human)
DOCK1 (human)
ENaC-beta (human)
ERas (human)
HRas (human)
KRas (human)
Lck (human)
MLC1 (human)
MST2 (human)
NDRG3 (human)
osteopontin (human)
PAK1 (human)
PHB (human)
PIN1 (human)
PKCE (human)
PLA2G7 (human)
PPP1R14B (human)
PPP5C (human)
RAF1 (human)
RHEB (human)
Src (human)

Treatment

A-443654
antibody
AzaC
BAPTA-AM
bradykinin
cell_detachment
colforsin
dasatinib
EGb 761
EGF
estrogen_deprivation
FGF1
fibronectin
FRAX597
genistein
GNF2
GTP-gammaS
GW_5074
H-89
IBMX
IFN-gamma
imatinib
insulin
LY294002
LY3009120
manumycin_A
methyl-beta-cyclodextrin
nilotinib
nocodazole
NSC23766
PAF
PD0325901
PDGF
PF562271
phorbol_ester
pictilisib
PKC412
proanthocyanidin
rapamycin
retinoic_acid
rigosertib
SCF
SCH772984
selumetinib
serum
siRNA
sorafenib
sotorasib
SU6656
TNF
U0126
vanadate
virus infection
wortmannin

RAF1 (human)	RPRGQRDssyyWEIE	S338-p
RAF1 iso2 (human)	RPRGQRDSSYYWEIE	S358
RAF1 (mouse)	RPRGQRDssyyWEIE	S338-p
RAF1 (rat)	RPRGQRDsSyyWEIE	S338-p
RAF1 (chicken)	RPRGQRDssyyWEIE	S338-p

S339-p

PRGQRDssyyWEIEA

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
intracellular localization
molecular association, regulation

Putative in vivo kinases:

PAK1 (human)

RAF1 (human)	PRGQRDssyyWEIEA	S339-p
RAF1 iso2 (human)	PRGQRDSSYYWEIEA	S359
RAF1 (mouse)	PRGQRDssyyWEIEA	S339-p
RAF1 (rat)	PRGQRDsSyyWEIEA	S339
RAF1 (chicken)	PRGQRDssyyWEIEA	S339-p

Y340-p

RGQRDssyyWEIEAS

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
enzymatic activity, inhibited
molecular association, regulation
phosphorylation

Kinase, in vitro:

Src (chicken)

Putative in vivo kinases:

Src (human)

Regulatory protein:

SHC1 (human)

Treatment

EGF
PKC412
retinoic_acid

RAF1 (human)	RGQRDssyyWEIEAS	Y340-p
RAF1 iso2 (human)	RGQRDSSYYWEIEAS	Y360
RAF1 (mouse)	RGQRDssyyWEIEAS	Y340-p
RAF1 (rat)	RGQRDsSyyWEIEAS	Y340-p
RAF1 (chicken)	RGQRDssyyWEIEAS	Y340-p

Y341-p

GQRDssyyWEIEASE

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
enzymatic activity, inhibited
intracellular localization
phosphorylation
protein conformation

Effects on Biological Processes:

carcinogenesis, induced
cell growth, induced
cell motility, induced
signaling pathway regulation

Kinase, in vitro:

Src (chicken)
Src (human)

Putative in vivo kinases:

Src (human)

Regulatory protein:

SHC1 (human)

Treatment

EGb 761
EGF
FGF1
gefitinib
PKC412
retinoic_acid
vanadate

RAF1 (human)	GQRDssyyWEIEASE	Y341-p
RAF1 iso2 (human)	GQRDSSYYWEIEASE	Y361
RAF1 (mouse)	GQRDssyyWEIEASE	Y341-p
RAF1 (rat)	GQRDsSyyWEIEASE	Y341-p
RAF1 (chicken)	GQRDssyyWEIEASE	Y341-p

T353-p

ASEVMLStRIGsGsF

RAF1 (human)	ASEVMLStRIGsGsF	T353-p
RAF1 iso2 (human)	ASEVMLSTRIGSGSF	T373
RAF1 (mouse)	ASEVMLSTRIGSGSF	T353
RAF1 (rat)	ASEVMLSTRIGSGSF	T353
RAF1 (chicken)	ASEVMLSTRIGSGSF	T353

S357-p

MLStRIGsGsFGTVY

RAF1 (human)	MLStRIGsGsFGTVY	S357-p
RAF1 iso2 (human)	MLSTRIGSGSFGTVY	S377
RAF1 (mouse)	MLSTRIGSGSFGTVY	S357
RAF1 (rat)	MLSTRIGSGSFGTVY	S357
RAF1 (chicken)	MLSTRIGSGSFGTVY	S357

S359-p

StRIGsGsFGTVYKG

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited

Putative in vivo kinases:

RAF1 (human)

Treatment

ATP
compound A

RAF1 (human)	StRIGsGsFGTVYKG	S359-p
RAF1 iso2 (human)	STRIGSGSFGTVYKG	S379
RAF1 (mouse)	STRIGSGSFGTVYKG	S359
RAF1 (rat)	STRIGSGSFGTVYKG	S359
RAF1 (chicken)	STRIGSGSFGTVYKG	S359

K378-ub

DVAVKILkVVDPTPE

RAF1 (human)	DVAVKILkVVDPTPE	K378-ub
RAF1 iso2 (human)	DVAVKILKVVDPTPE	K398
RAF1 (mouse)	DVAVKILKVVDPTPE	K378
RAF1 (rat)	DVAVKILKVVDPTPE	K378
RAF1 (chicken)	DVAVKILKVVDPTPE	K378

K470-ub

NIIHRDMksNNIFLH

RAF1 (human)	NIIHRDMksNNIFLH	K470-ub
RAF1 iso2 (human)	NIIHRDMKSNNIFLH	K490
RAF1 (mouse)	NIIHRDMKSNNIFLH	K470
RAF1 (rat)	NIIHRDMKSNNIFLH	K470
RAF1 (chicken)	NIIHRDMKSNNIFLH	K470

S471-p

IIHRDMksNNIFLHE

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
molecular association, regulation

Treatment

EGF
U0126

RAF1 (human)	IIHRDMksNNIFLHE	S471-p
RAF1 iso2 (human)	IIHRDMKSNNIFLHE	S491
RAF1 (mouse)	IIHRDMKSNNIFLHE	S471
RAF1 (rat)	IIHRDMKSNNIFLHE	S471
RAF1 (chicken)	IIHRDMKSNNIFLHE	S471

T481-p

IFLHEGLtVKIGDFG

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Treatment

EGF
U0126

RAF1 (human)	IFLHEGLtVKIGDFG	T481-p
RAF1 iso2 (human)	IFLHEGLTVKIGDFG	T501
RAF1 (mouse)	IFLHEGLTVKIGDFG	T481
RAF1 (rat)	IFLHEGLTVKIGDFG	T481
RAF1 (chicken)	IFLHEGLTVKIGDFG	T481

T491-p

IGDFGLAtVksRWsG

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Regulatory protein:

HRas (human)
MLK3 (human)
RAF1 (human)

Treatment

EGF
serum
siRNA

RAF1 (human)	IGDFGLAtVksRWsG	T491-p
RAF1 iso2 (human)	IGDFGLATVKSRWSG	T511
RAF1 (mouse)	IGDFGLAtVKsRWsG	T491-p
RAF1 (rat)	IGDFGLATVKSRWSG	T491
RAF1 (chicken)	IGDFGLATVKSRWSG	T491

K493-ub

DFGLAtVksRWsGsQ

RAF1 (human)	DFGLAtVksRWsGsQ	K493-ub
RAF1 iso2 (human)	DFGLATVKSRWSGSQ	K513
RAF1 (mouse)	DFGLAtVKsRWsGSQ	K493
RAF1 (rat)	DFGLATVKSRWSGSQ	K493
RAF1 (chicken)	DFGLATVKSRWSGSQ	K493

S494-p

FGLAtVksRWsGsQQ

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Regulatory protein:

MLK3 (human)

Treatment

EGF
siRNA

RAF1 (human)	FGLAtVksRWsGsQQ	S494-p
RAF1 iso2 (human)	FGLATVKSRWSGSQQ	S514
RAF1 (mouse)	FGLAtVKsRWsGSQQ	S494-p
RAF1 (rat)	FGLATVKSRWSGSQQ	S494
RAF1 (chicken)	FGLATVKSRWSGSQQ	S494

S497-p

AtVksRWsGsQQVEQ

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Kinase, in vitro:

PKCA (rat)

Putative in vivo kinases:

PKCA (mouse)

Treatment

phorbol_ester

RAF1 (human)	AtVksRWsGsQQVEQ	S497-p
RAF1 iso2 (human)	ATVKSRWSGSQQVEQ	S517
RAF1 (mouse)	AtVKsRWsGSQQVEQ	S497-p
RAF1 (rat)	ATVKSRWSGSQQVEQ	S497
RAF1 (chicken)	ATVKSRWSGSQQVEQ	S497

S499-p

VksRWsGsQQVEQPT

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
phosphorylation

Kinase, in vitro:

PKCA (human)

Putative in vivo kinases:

PKCA (human)

Treatment

phorbol_ester

RAF1 (human)	VksRWsGsQQVEQPT	S499-p
RAF1 iso2 (human)	VKSRWSGSQQVEQPT	S519
RAF1 (mouse)	VKsRWsGSQQVEQPT	S499
RAF1 (rat)	VKSRWSGSQQVEQPT	S499
RAF1 (chicken)	VKSRWSGSQQVEQPT	S499

K575-ub

PDLSKLYkNCPKAMK

RAF1 (human)	PDLSKLYkNCPKAMK	K575-ub
RAF1 iso2 (human)	PDLSKLYKNCPKAMK	K595
RAF1 (mouse)	PDLSRLYKNCPKAMK	K575
RAF1 (rat)	PDLSRLYKNCPKAMK	K575
RAF1 (chicken)	PDLSKLYKNCPKAMK	K575

S612-p

SIELLQHsLPKINRs

RAF1 (human)	SIELLQHsLPKINRs	S612-p
RAF1 iso2 (human)	SIELLQHSLPKINRS	S632
RAF1 (mouse)	SIELLQHsLPKINRs	S612-p
RAF1 (rat)	SIELLQHSLPKINRS	S612
RAF1 (chicken)	SIELLQHSLPKINRS	S612

S619-p

sLPKINRsAsEPsLH

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Kinase, in vitro:

PKCA (rat)

Putative in vivo kinases:

PKCA (mouse)

RAF1 (human)	sLPKINRsAsEPsLH	S619-p
RAF1 iso2 (human)	SLPKINRSASEPSLH	S639
RAF1 (mouse)	sLPKINRsAsEPsLH	S619-p
RAF1 (rat)	SLPKINRSAsEPSLH	S619
RAF1 (chicken)	SLPKINRSASEPSLH	S619

S621-p

PKINRsAsEPsLHRA

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
enzymatic activity, inhibited
molecular association, regulation
phosphorylation
protein conformation

Effects on Biological Processes:

cell growth, altered

Kinase, in vitro:

AMPKA1 (mouse)
PKACA (human)
PKACA (mouse)
RAF1 (human)

Putative in vivo kinases:

PKACA (mouse)
RAF1 (human)

Treatment

colforsin
EGF
M-CSF
metastatic potential
MG132_withdrawal
okadaic_acid
PDGF
phorbol_ester
retinoic_acid

RAF1 (human)	PKINRsAsEPsLHRA	S621-p
RAF1 iso2 (human)	PKINRSASEPSLHRA	S641
RAF1 (mouse)	PKINRsAsEPsLHRA	S621-p
RAF1 (rat)	PKINRSAsEPSLHRA	S621-p
RAF1 (chicken)	PKINRSASEPSLHRA	S621

S624-p

NRsAsEPsLHRAAHT

Downstream

Effects on Modified Protein

molecular association, regulation
phosphorylation

RAF1 (human)	NRsAsEPsLHRAAHT	S624-p
RAF1 iso2 (human)	NRSASEPSLHRAAHT	S644
RAF1 (mouse)	NRsAsEPsLHRAAHT	S624-p
RAF1 (rat)	NRSAsEPSLHRAAHT	S624
RAF1 (chicken)	NRSASEPSLHRASHT	S624

T638-p

TEDINACtLtTsPRL

RAF1 (human)	TEDINACtLtTsPRL	T638-p
RAF1 iso2 (human)	TEDINACTLTTSPRL	T658
RAF1 (mouse)	TEDINACTLTtsPRL	T638
RAF1 (rat)	TEDINACTLTTSPRL	T638
RAF1 (chicken)	TEDINSCTLTSTRLP	T638

T640-p

DINACtLtTsPRLPV

RAF1 (human)	DINACtLtTsPRLPV	T640-p
RAF1 iso2 (human)	DINACTLTTSPRLPV	T660
RAF1 (mouse)	DINACTLTtsPRLPV	T640
RAF1 (rat)	DINACTLTTSPRLPV	T640
RAF1 (chicken)	gap	-

T641

INACtLtTsPRLPVF

RAF1 (human)	INACtLtTsPRLPVF	T641
RAF1 iso2 (human)	INACTLTTSPRLPVF	T661
RAF1 (mouse)	INACTLTtsPRLPVF	T641-p
RAF1 (rat)	INACTLTTSPRLPVF	T641
RAF1 (chicken)	DINSCTLTSTRLPVF	T640

S642-p

NACtLtTsPRLPVF_

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation

Regulatory protein:

CD38 (human)

Treatment

okadaic_acid
PDGF
selumetinib
U0126
vemurafenib

RAF1 (human)	NACtLtTsPRLPVF_	S642-p
RAF1 iso2 (human)	NACTLTTSPRLPVF_	S662
RAF1 (mouse)	NACTLTtsPRLPVF_	S642-p
RAF1 (rat)	NACTLTTSPRLPVF_	S642
RAF1 (chicken)	INSCTLTSTRLPVF_	S641

T543-p

IVLYELMtGELPYSH

RAF1 (human)	IVLYELMtGELPYSH	T543-p
RAF1 iso2 (human)	IVLYELMTGELPYSH	T563
RAF1 (mouse)	IVLYELMAGELPYAH	A543
RAF1 (rat)	IVLYELMTGELPYSH	T543
RAF1 (chicken)	IVLYELMTGELPYSH	T543