PLK1 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

______MsAAVtAGK

PLK1 (human)	______MsAAVtAGK	S2
PLK1 (mouse)	______MNAAAKAGK	N2
PLK1 (rat)	______MNAAAKAGK	N2
PLK1 (frog)	gap	-

__MsAAVtAGKLARA

Upstream

Treatment

MG132_withdrawal
nocodazole
thymidine

PLK1 (human)	__MsAAVtAGKLARA	T6
PLK1 (mouse)	__MNAAAKAGKLARA	K6
PLK1 (rat)	__MNAAAKAGKLARA	K6
PLK1 (frog)	___MAQVAGKKLTVA	A5

A34

APGAPAAAPPAKEIP

PLK1 (human)	APGAPAAAPPAKEIP	A34
PLK1 (mouse)	VPGAPVAAPLAKEIP	A34
PLK1 (rat)	APGAPGAAPLAKEIP	A34
PLK1 (frog)	PPGIPGSssAVKEIP	S25

P35

PGAPAAAPPAKEIPE

PLK1 (human)	PGAPAAAPPAKEIPE	P35
PLK1 (mouse)	PGAPVAAPLAKEIPE	P35
PLK1 (rat)	PGAPGAAPLAKEIPE	P35
PLK1 (frog)	PGIPGSssAVKEIPE	S26

S49

EVLVDPRsRRRYVRG

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

PAK1 (human)

Putative in vivo kinases:

PAK1 (human)

PLK1 (human)	EVLVDPRsRRRYVRG	S49
PLK1 (mouse)	EVLVDPRSRRQYVRG	S49
PLK1 (rat)	EVLMDPRSRRQYVRG	S49
PLK1 (frog)	EILVDPRTRRRYLRG	T40

S99

PHQREKMsMEIsIHR

Upstream

Downstream

Effects on Biological Processes:

cell cycle regulation

Treatment

sphingosine_1-phosphate

PLK1 (human)	PHQREKMsMEIsIHR	S99
PLK1 (mouse)	PHQKEKMSMEISIHR	S99
PLK1 (rat)	PHQKEKMSMEISIHR	S99
PLK1 (frog)	PHQKDKMTMEIAIQR	T90

S103

EKMsMEIsIHRSLAH

Upstream

Treatment

nocodazole
thymidine

PLK1 (human)	EKMsMEIsIHRSLAH	S103
PLK1 (mouse)	EKMSMEISIHRSLAH	S103
PLK1 (rat)	EKMSMEISIHRSLAH	S103
PLK1 (frog)	DKMTMEIAIQRSLDH	A94

S137

LELCRRRsLLELHKR

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
intracellular localization
molecular association, regulation
phosphorylation

Effects on Biological Processes:

DNA repair, induced
apoptosis, inhibited
cell cycle regulation
cell growth, induced

Kinase, in vitro:

Chk1 (human)
Cot (human)
PLK2 (human)

Putative in vivo kinases:

Chk1 (human)
Cot (human)
PLK2 (human)

Regulatory protein:

VRK2 (human)

Treatment

adriamycin
CPT
gallotannin
ionizing_radiation
MG132
nocodazole
olaparib
UCN-01

PLK1 (human)	LELCRRRsLLELHKR	S137
PLK1 (mouse)	LELCRRRsLLELHKR	S137
PLK1 (rat)	LELCRRRSLLELHKR	S137
PLK1 (frog)	LELCRRRSLLELHKR	S128

Y166

QIVLGCQYLHRNRVI

PLK1 (human)	QIVLGCQYLHRNRVI	Y166
PLK1 (mouse)	QIVLGCQyLHRNQVI	Y166
PLK1 (rat)	QIVLGCQYLHRNQVI	Y166
PLK1 (frog)	QTISGCQYLHSNRVI	Y157

T210

YDGERKKtLCGtPNy

Upstream

Downstream

PLK1 (human)
T210

PLK1 (mouse)
T210

PLK1 (rat)
T210

Effects on Modified Protein

enzymatic activity, induced
intracellular localization
molecular association, regulation
phosphorylation
protein stabilization
ubiquitination

Effects on Biological Processes:

DNA repair, induced
autophagy, induced
carcinogenesis, induced
carcinogenesis, inhibited
cell cycle regulation
cell growth, induced
cell motility, induced
chromatin organization, altered
cytoskeletal reorganization
transcription, altered

Kinase, in vitro:

AurA (human)
AurB (human)
Chk1 (human)
Cot (human)
LOK (human)
VRK2 (human)

Phosphatases, in vitro:

MYPT1 (human)
PPP1CA (human)
PPP1CC (human)

Putative in vivo kinases:

AurA (human)
Chk1 (human)
PLK1 (human)
VRK2 (human)

Putative upstream phosphatases:

MYPT1 (human)
PPP1CA (human)

Regulatory protein:

Abl (human)
APOA1BP (human)
ARPC1B (human)
ARPP-19 (human)
BORA (human)
DAB2IP (human)
DNAPK (human)
FIRRM (human)
MYPT1 (human)
nestin (human)
optineurin (human)
PARP10 (human)
PLK1 (human)
PPP6C (human)
SLK (human)
WAC (human)

Treatment

ACh
adriamycin
alpha2-macroglobulin
antimycin_A
arsenite
Aurora_A_Inhibitor_I
auxin
BI2536
BI_4834
BIX01294
BX795
caffeine
doxycycline
EDTA
etoposide
gallotannin
imatinib
KU-55933
MG132
MLN8054
MLN8237
nocodazole
NU7441
okadaic_acid
PDGF
RO-3306
Ro3280
rotenone
SB431542
siRNA
sphingosine_1-phosphate
TAME
taxol
TGF-beta
thymidine
ZM447439

PLK1 (human)	YDGERKKtLCGtPNy	T210
PLK1 (mouse)	YEGERKKtLCGtPNY	T210
PLK1 (rat)	YEGERKKtLCGTPNY	T210
PLK1 (frog)	YDGERKKTLCGTPNY	T201

T214

RKKtLCGtPNyIAPE

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Effects on Biological Processes:

cell cycle regulation
cell growth, induced

Treatment

CXCL12
MG132
MLN8054
nocodazole
thymidine

PLK1 (human)	RKKtLCGtPNyIAPE	T214
PLK1 (mouse)	RKKtLCGtPNYIAPE	T214
PLK1 (rat)	RKKtLCGTPNYIAPE	T214
PLK1 (frog)	RKKTLCGTPNYIAPE	T205

Y217

tLCGtPNyIAPEVLs

Upstream

150

Kinase, in vitro:

Abl (human)

Putative in vivo kinases:

Abl (human)

PLK1 (human)	tLCGtPNyIAPEVLs	Y217
PLK1 (mouse)	tLCGtPNYIAPEVLS	Y217
PLK1 (rat)	tLCGTPNYIAPEVLS	Y217
PLK1 (frog)	TLCGTPNYIAPEVLG	Y208

S224

yIAPEVLsKKGHSFE

Upstream

Treatment

AZD1152
ZM447439

PLK1 (human)	yIAPEVLsKKGHSFE	S224
PLK1 (mouse)	YIAPEVLSKKGHSFE	S224
PLK1 (rat)	YIAPEVLSKKGHSFE	S224
PLK1 (frog)	YIAPEVLGKKGHSFE	G215

Y309

DEFFTSGyIPARLPI

PLK1 (human)	DEFFTSGyIPARLPI	Y309
PLK1 (mouse)	DEFFTSGYIPARLPI	Y309
PLK1 (rat)	DEFFTSGYIPARLPI	Y309
PLK1 (frog)	DEFFTSGYIPSRLPT	Y300

S326

LTIPPRFsIAPssLD

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

MAPKAPK2 (human)

Putative in vivo kinases:

MAPKAPK2 (human)

Treatment

nocodazole
seliciclib
ZK-Thiazolidinone

PLK1 (human)	LTIPPRFsIAPssLD	S326
PLK1 (mouse)	LTIPPRFSIAPSSLD	S326
PLK1 (rat)	LTIPPRFSIAPSSLD	S326
PLK1 (frog)	LTVPPRFSIAPSTID	S317

S330

PRFsIAPssLDPsNR

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

cell cycle regulation

Treatment

okadaic_acid

PLK1 (human)	PRFsIAPssLDPsNR	S330
PLK1 (mouse)	PRFSIAPSSLDPSSR	S330
PLK1 (rat)	PRFSIAPSSLDPSNR	S330
PLK1 (frog)	PRFSIAPSTIDQsLR	S321

S331

RFsIAPssLDPsNRK

Upstream

Treatment

MG132_withdrawal

PLK1 (human)	RFsIAPssLDPsNRK	S331
PLK1 (mouse)	RFSIAPSSLDPSSRK	S331
PLK1 (rat)	RFSIAPSSLDPSNRK	S331
PLK1 (frog)	RFSIAPSTIDQsLRK	T322

S335

APssLDPsNRKPLtV

Upstream

Treatment

okadaic_acid

PLK1 (human)	APssLDPsNRKPLtV	S335
PLK1 (mouse)	APSSLDPSSRKPLKV	S335
PLK1 (rat)	APSSLDPSNRKPLTV	S335
PLK1 (frog)	APSTIDQsLRKPLTA	S326

T341

PsNRKPLtVLNKGLE

PLK1 (human)	PsNRKPLtVLNKGLE	T341
PLK1 (mouse)	PSSRKPLKVLNKGVE	K341
PLK1 (rat)	PSNRKPLTVLNKGVE	T341
PLK1 (frog)	QsLRKPLTAINKGQD	T332

N349

VLNKGLENPLPERPR

PLK1 (human)	VLNKGLENPLPERPR	N349
PLK1 (mouse)	VLNKGVENPLPDRPR	N349
PLK1 (rat)	VLNKGVENPLPDRPR	N349
PLK1 (frog)	AINKGQDsPLVEKQV	S340

T366

EEPVVREtGEVVDCH

PLK1 (human)	EEPVVREtGEVVDCH	T366
PLK1 (mouse)	EEPVVRETNEAIECH	T366
PLK1 (rat)	EEPVVRETNEAIECH	T366
PLK1 (frog)	EEMQQPEFTEPADCY	F360

S375

EVVDCHLsDMLQQLH

Upstream

Treatment

nocodazole
thymidine

PLK1 (human)	EVVDCHLsDMLQQLH	S375
PLK1 (mouse)	EAIECHLSDLLQQLT	S375
PLK1 (rat)	EAIECHLSDLLQQLT	S375
PLK1 (frog)	EPADCYLSEMLQQLT	S369

S383

DMLQQLHsVNAsKPS

Upstream

Kinase, in vitro:

MAPKAPK2 (human)

PLK1 (human)	DMLQQLHsVNAsKPS	S383
PLK1 (mouse)	DLLQQLTSVNASKPS	S383
PLK1 (rat)	DLLQQLTSVNASKPS	S383
PLK1 (frog)	EMLQQLTCLNAVKPS	C377

S387

QLHsVNAsKPSERGL

PLK1 (human)	QLHsVNAsKPSERGL	S387
PLK1 (mouse)	QLTSVNASKPSERGL	S387
PLK1 (rat)	QLTSVNASKPSERGL	S387
PLK1 (frog)	QLTCLNAVKPSERAL	V381

Y425

SDKYGLGyQLCDNSV

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein stabilization

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

Abl (human)

Putative in vivo kinases:

Abl (human)

PLK1 (human)	SDKYGLGyQLCDNSV	Y425
PLK1 (mouse)	SDKYGLGYQLCDNSV	Y425
PLK1 (rat)	SDKYGLGYQLCDNSV	Y425
PLK1 (frog)	SDKYGLGYQLCDNSV	Y419

Y445

DSTRLILyNDGDsLQ

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein conformation

Effects on Biological Processes:

apoptosis, induced
cell cycle regulation
cytoskeletal reorganization

Kinase, in vitro:

Abl (human)

Phosphatases, in vitro:

EYA1 (human)
EYA3 (human)
EYA4 (human)

Putative in vivo kinases:

Abl (human)

Putative upstream phosphatases:

EYA4 (human)

Treatment

Benzarone
siRNA

PLK1 (human)	DSTRLILyNDGDsLQ	Y445
PLK1 (mouse)	DSTRLILyNDGDSLQ	Y445
PLK1 (rat)	DSTRLILYNDGDSLQ	Y445
PLK1 (frog)	DSTRLIMYNDGDSLQ	Y439

S450

ILyNDGDsLQYIERD

Upstream

Treatment

nocodazole
thymidine

PLK1 (human)	ILyNDGDsLQYIERD	S450
PLK1 (mouse)	ILyNDGDSLQYIERD	S450
PLK1 (rat)	ILYNDGDSLQYIERD	S450
PLK1 (frog)	IMYNDGDSLQYIERN	S444

T459

QYIERDGtEsyLtVs

PLK1 (human)	QYIERDGtEsyLtVs	T459
PLK1 (mouse)	QYIERDGTESYLTVS	T459
PLK1 (rat)	QYIERDGTESYLTVS	T459
PLK1 (frog)	QYIERNNTESYLNVR	T453

S461

IERDGtEsyLtVsSH

Upstream

Treatment

nocodazole
thymidine

PLK1 (human)	IERDGtEsyLtVsSH	S461
PLK1 (mouse)	IERDGTESYLTVSSH	S461
PLK1 (rat)	IERDGTESYLTVSSH	S461
PLK1 (frog)	IERNNTESYLNVRSY	S455

Y462

ERDGtEsyLtVsSHP

PLK1 (human)	ERDGtEsyLtVsSHP	Y462
PLK1 (mouse)	ERDGTESYLTVSSHP	Y462
PLK1 (rat)	ERDGTESYLTVSSHP	Y462
PLK1 (frog)	ERNNTESYLNVRSYP	Y456

T464

DGtEsyLtVsSHPNS

Upstream

Treatment

nocodazole
thymidine

PLK1 (human)	DGtEsyLtVsSHPNS	T464
PLK1 (mouse)	DGTESYLTVSSHPNS	T464
PLK1 (rat)	DGTESYLTVSSHPNS	T464
PLK1 (frog)	NNTESYLNVRSYPTT	N458

S466

tEsyLtVsSHPNSLM

PLK1 (human)	tEsyLtVsSHPNSLM	S466
PLK1 (mouse)	TESYLTVSSHPNSLM	S466
PLK1 (rat)	TESYLTVSSHPNSLM	S466
PLK1 (frog)	TESYLNVRSYPTTLT	R460

T498

LKAGANItPREGDEL

Upstream

Treatment

nocodazole
thymidine

PLK1 (human)	LKAGANItPREGDEL	T498
PLK1 (mouse)	LKAGANITPREGDEL	T498
PLK1 (rat)	LKAGANITPREGDEL	T498
PLK1 (frog)	LKAGANTTPREGDEL	T492

S526

SAIILHLsNGsVQIN

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein conformation

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

AurA (human)

Putative in vivo kinases:

AurA (human)

Regulatory protein:

HRNR (human)

Treatment

BI2536
BPDE
hesperadin
nocodazole
NU6027
RO-3306
UCN-01
VX-680

PLK1 (human)	SAIILHLsNGsVQIN	S526
PLK1 (mouse)	SAIILHLSNGTVQIN	S526
PLK1 (rat)	SAIILHLSNGTVQIN	S526
PLK1 (frog)	SAIILHLSNGTVQIN	S520

S529

ILHLsNGsVQINFFQ

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein conformation

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

Chk1 (human)

Putative in vivo kinases:

Chk1 (human)

Regulatory protein:

HRNR (human)

Treatment

BPDE
NU6027
RO-3306
UCN-01

PLK1 (human)	ILHLsNGsVQINFFQ	S529
PLK1 (mouse)	ILHLSNGTVQINFFQ	T529
PLK1 (rat)	ILHLSNGTVQINFFQ	T529
PLK1 (frog)	ILHLSNGTVQINFFQ	T523

T539

INFFQDHtKLILCPL

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein conformation

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

Chk1 (human)

Putative in vivo kinases:

Chk1 (human)

Regulatory protein:

HRNR (human)

Treatment

BPDE
NU6027
RO-3306
UCN-01

PLK1 (human)	INFFQDHtKLILCPL	T539
PLK1 (mouse)	INFFQDHTKLILCPL	T539
PLK1 (rat)	INFFQDHTKLILCPL	T539
PLK1 (frog)	INFFQDHTKIILCPL	T533

S565

DFRTYRLsLLEEYGC

PLK1 (human)	DFRTYRLsLLEEYGC	S565
PLK1 (mouse)	DFQTYRLSLLEEYGC	S565
PLK1 (rat)	DFRTYRLSLLEEYGC	S565
PLK1 (frog)	EFRTYKLSLIQEFGC	S559

S578

GCCKELAsRLRYART

PLK1 (human)	GCCKELAsRLRYART	S578
PLK1 (mouse)	GCCKELASRLRYART	S578
PLK1 (rat)	GCCKELASRLRYART	S578
PLK1 (frog)	GCCKELASRLRYART	S572

S597

LLSSRSAsNRLKAS_

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

cell cycle regulation

Treatment

okadaic_acid

PLK1 (human)	LLSSRSAsNRLKAS_	S597
PLK1 (mouse)	LLSSRSASNRLKAS_	S597
PLK1 (rat)	LLSSRSACNRLKAS_	C597
PLK1 (frog)	LQSSKSAVAHVKASA	V591