Chk1 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

K43-ub

AVKIVDMkRAVDCPE

Chk1 (human)	AVKIVDMkRAVDCPE	K43-ub
Chk1 (mouse)	AVKIVDMKRAIDCPE	K43
Chk1 (rat)	AVKIVDMKRAIDCPE	K43
Chk1 (chicken)	AVKIVDMKRAADCPE	K43

K53-ac

VDCPENIkkEICINk

Chk1 (human)	VDCPENIkkEICINk	K53-ac
Chk1 (mouse)	IDCPENIKKEICINK	K53
Chk1 (rat)	IDCPENIKKEICINK	K53
Chk1 (chicken)	ADCPENIKKEICINK	K53

K53-ub

VDCPENIkkEICINk

Chk1 (human)	VDCPENIkkEICINk	K53-ub
Chk1 (mouse)	IDCPENIKKEICINK	K53
Chk1 (rat)	IDCPENIKKEICINK	K53
Chk1 (chicken)	ADCPENIKKEICINK	K53

K54-ub

DCPENIkkEICINkM

Chk1 (human)	DCPENIkkEICINkM	K54-ub
Chk1 (mouse)	DCPENIKKEICINKM	K54
Chk1 (rat)	DCPENIKKEICINKM	K54
Chk1 (chicken)	DCPENIKKEICINKM	K54

K60-ub

kkEICINkMLNHENV

Chk1 (human)	kkEICINkMLNHENV	K60-ub
Chk1 (mouse)	KKEICINKMLSHENV	K60
Chk1 (rat)	KKEICINKMLNHENV	K60
Chk1 (chicken)	KKEICINKMLNHENV	K60

K132-ub

GITHRDIkPENLLLD

Downstream

Effects on Modified Protein

enzymatic activity, induced
phosphorylation

Chk1 (human)	GITHRDIkPENLLLD	K132-ub
Chk1 (mouse)	GITHRDIKPENLLLD	K132
Chk1 (rat)	GITHRDIKPENLLLD	K132
Chk1 (chicken)	GITHRDLKPENLLLD	K132

K145-ub

LDERDNLkISDFGLA

Chk1 (human)	LDERDNLkISDFGLA	K145-ub
Chk1 (mouse)	LDERDNLKISDFGLA	K145
Chk1 (rat)	LDERDNLKISDFGLA	K145
Chk1 (chicken)	LDERDNLKISDFGLA	K145

K166-ub

NRERLLNkMCGTLPY

Chk1 (human)	NRERLLNkMCGTLPY	K166-ub
Chk1 (mouse)	NRERLLNKMCGTLPY	K166
Chk1 (rat)	NRERLLNKMCGTLPY	K166
Chk1 (chicken)	GRERLLNKMCGTLPY	K166

K180-ub

YVAPELLkRREFHAE

Chk1 (human)	YVAPELLkRREFHAE	K180-ub
Chk1 (mouse)	YVAPELLKRKEFHAE	K180
Chk1 (rat)	YVAPELLKRKEFHAE	K180
Chk1 (chicken)	YVAPELLRRPEFRAE	R180

K225-ub

YSDWKEKkTYLNPWK

Chk1 (human)	YSDWKEKkTYLNPWK	K225-ub
Chk1 (mouse)	YSDWKEKKTYLNPWK	K225
Chk1 (rat)	YSDWKEKKTYLNPWK	K225
Chk1 (chicken)	YSDWKERKTYLAPWR	K225

K233-ub

TYLNPWKkIDSAPLA

Chk1 (human)	TYLNPWKkIDSAPLA	K233-ub
Chk1 (mouse)	TYLNPWKKIDSAPLA	K233
Chk1 (rat)	TYLNPWKKIDSAPLA	K233
Chk1 (chicken)	TYLAPWRKIDSAPLA	K233

K244-ub

APLALLHkILVENPS

Chk1 (human)	APLALLHkILVENPS	K244-ub
Chk1 (mouse)	APLALLHKILVETPS	K244
Chk1 (rat)	APLALLHKILVENPS	K244
Chk1 (chicken)	APLALLHKILTENPT	K244

K267-ub

KKDRWYNkPLKKGAK

Chk1 (human)	KKDRWYNkPLKKGAK	K267-ub
Chk1 (mouse)	KKDRWYNKPLNRGAK	K267
Chk1 (rat)	KKDRWYNKPLNRGAK	K267
Chk1 (chicken)	KKDRWYCRPLKKGTK	R267

T279-p

GAKRPRVtsGGVsEs

Chk1 (human)	GAKRPRVtsGGVsEs	T279-p
Chk1 (mouse)	GAKRPRATsGGMSEs	T279
Chk1 (rat)	GAKRPRATSGGMSES	T279
Chk1 (chicken)	GTKRGRVSSGGVTEs	S279

S280-p

AKRPRVtsGGVsEsP

Upstream

Downstream

170

Chk1 (human)
S280-p

2347 : Phospho-Chk1(Ser280) Antibody

Effects on Modified Protein

enzymatic activity, induced
enzymatic activity, inhibited
intracellular localization
molecular association, regulation
protein degradation

Effects on Biological Processes:

DNA repair, inhibited
carcinogenesis, induced
cell cycle regulation

Kinase, in vitro:

Akt1 (human)
p90RSK (human)

Putative in vivo kinases:

Akt1 (human)
p90RSK (human)

Regulatory protein:

Akt1 (human)
COX-2 (human)
HRas (human)
MEK1 (human)

Treatment

2-deoxyglucose
anisomycin
antibody
BI-D1870
camptothecin
cisplatin
EGF
erlotinib
estradiol
hydroxyurea
IGF-1
insulin
ionizing_radiation
LPA
LY294002
MK-2206
PD184352
phorbol_ester
rapamycin
SB203580
selumetinib
serum
siRNA
SL0101
Su11274
TMZ
U0126
UV
vemurafenib
wortmannin

Chk1 (human)	AKRPRVtsGGVsEsP	S280-p
Chk1 (mouse)	AKRPRATsGGMSEsS	S280-p
Chk1 (rat)	AKRPRATSGGMSESS	S280
Chk1 (chicken)	TKRGRVSSGGVTEsP	S280

S284-p

RVtsGGVsEsPsGFs

Chk1 (human)	RVtsGGVsEsPsGFs	S284-p
Chk1 (mouse)	RATsGGMSEsSSGFS	S284
Chk1 (rat)	RATSGGMSESSSGFS	S284
Chk1 (chicken)	RVSSGGVTEsPGALP	T284

S286-p

tsGGVsEsPsGFskH

Upstream

Downstream

Effects on Modified Protein

intracellular localization

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

CDK1 (human)
CDK2 (human)

Treatment

hydroxyurea
MG132_withdrawal
nocodazole
PD98059
purvalanol
SB203580
seliciclib
SP600125
thymidine
UV

Chk1 (human)	tsGGVsEsPsGFskH	S286-p
Chk1 (mouse)	TsGGMSEsSSGFSKH	S286-p
Chk1 (rat)	TSGGMSESSSGFSKH	S286
Chk1 (chicken)	SSGGVTEsPGALPKH	S286-p

S288-p

GGVsEsPsGFskHIQ

Upstream

Treatment

MG132_withdrawal

Chk1 (human)	GGVsEsPsGFskHIQ	S288-p
Chk1 (mouse)	GGMSEsSSGFSKHIH	S288
Chk1 (rat)	GGMSESSSGFSKHIH	S288
Chk1 (chicken)	GGVTEsPGALPKHIR	G288

S291-p

sEsPsGFskHIQsNL

Upstream

Kinase, in vitro:

Chk1 (human)

Chk1 (human)	sEsPsGFskHIQsNL	S291-p
Chk1 (mouse)	SEsSSGFSKHIHsNL	S291
Chk1 (rat)	SESSSGFSKHIHSNL	S291
Chk1 (chicken)	TEsPGALPKHIRSDT	P291

K292-ub

EsPsGFskHIQsNLD

Chk1 (human)	EsPsGFskHIQsNLD	K292-ub
Chk1 (mouse)	EsSSGFSKHIHsNLD	K292
Chk1 (rat)	ESSSGFSKHIHSNLD	K292
Chk1 (chicken)	EsPGALPKHIRSDTD	K292

S296-p

GFskHIQsNLDFsPV

Upstream

Downstream

Chk1 (human)
S296-p

Chk1 (mouse)
S296-p

Chk1 (rat)
S296-p

Effects on Modified Protein

activity, induced
enzymatic activity, induced
intracellular localization
molecular association, regulation

Effects on Biological Processes:

apoptosis, inhibited
carcinogenesis, induced
cell cycle regulation
cell growth, altered
cell growth, induced
signaling pathway regulation
transcription, induced
transcription, inhibited

Kinase, in vitro:

ATR (human)
Chk1 (human)

Putative in vivo kinases:

Chk1 (human)

Regulatory protein:

N-Myc (human)
p21Cip1 (human)

Treatment

actinomycin_D
adriamycin
aphidicolin
AZD6738
AZD7762
BI-D1870
caffeine
camptothecin
compound 13
cytarabine
evofosfamide
gemcitabine
Go_6976
hydroxyurea
hypoxia
ionizing_radiation
KU-55933
mitomycin_C
MK-8776
olaparib
PF477736
PHA-767491
prexasertib
rabusertib
serum
siRNA
SN-38
THIF
thymidine
UCN-01
UV

Chk1 (human)	GFskHIQsNLDFsPV	S296-p
Chk1 (mouse)	GFSKHIHsNLDFsPV	S296-p
Chk1 (rat)	GFSKHIHSNLDFSPI	S296
Chk1 (chicken)	ALPKHIRSDTDFsPV	S296

S301-p

IQsNLDFsPVNsAss

Upstream

Downstream

Effects on Modified Protein

intracellular localization

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

CDK1 (human)
CDK2 (human)

Putative in vivo kinases:

CDK1 (human)

Regulatory protein:

CDK1 (human)
CDK2 (human)

Treatment

1NM-PP1
aphidicolin
hydroxyurea
nocodazole
PD98059
purvalanol
SB203580
seliciclib
SP600125
UV

Chk1 (human)	IQsNLDFsPVNsAss	S301-p
Chk1 (mouse)	IHsNLDFsPVNNGSs	S301-p
Chk1 (rat)	IHSNLDFSPINSGSS	S301
Chk1 (chicken)	IRSDTDFsPVKSALG	S301-p

S305-p

LDFsPVNsAssEENV

Chk1 (human)	LDFsPVNsAssEENV	S305-p
Chk1 (mouse)	LDFsPVNNGSsEETV	N305
Chk1 (rat)	LDFSPINSGSSEENV	S305
Chk1 (chicken)	TDFsPVKSALGEDKA	S305

S307-p

FsPVNsAssEENVkY

Chk1 (human)	FsPVNsAssEENVkY	S307-p
Chk1 (mouse)	FsPVNNGSsEETVKF	S307
Chk1 (rat)	FSPINSGSSEENVKF	S307
Chk1 (chicken)	FsPVKSALGEDKASY	L307

S308-p

sPVNsAssEENVkYs

Chk1 (human)	sPVNsAssEENVkYs	S308-p
Chk1 (mouse)	sPVNNGSsEETVKFs	S308-p
Chk1 (rat)	SPINSGSSEENVKFS	S308
Chk1 (chicken)	sPVKSALGEDKASYS	G308

K313-ub

AssEENVkYsssQPE

Chk1 (human)	AssEENVkYsssQPE	K313-ub
Chk1 (mouse)	GSsEETVKFsSsQPE	K313
Chk1 (rat)	GSSEENVKFSSSQPE	K313
Chk1 (chicken)	ALGEDKASYSTSQPE	S313

S315-p

sEENVkYsssQPEPR

Chk1 (human)	sEENVkYsssQPEPR	S315-p
Chk1 (mouse)	sEETVKFsSsQPEPR	S315-p
Chk1 (rat)	SEENVKFSSSQPEPR	S315
Chk1 (chicken)	GEDKASYSTSQPEPG	S315

S316-p

EENVkYsssQPEPRT

Upstream

Kinase, in vitro:

Chk1 (human)

Chk1 (human)	EENVkYsssQPEPRT	S316-p
Chk1 (mouse)	EETVKFsSsQPEPRT	S316
Chk1 (rat)	EENVKFSSSQPEPRT	S316
Chk1 (chicken)	EDKASYSTSQPEPGT	T316

S317-p

ENVkYsssQPEPRTG

Upstream

Downstream

Chk1 (human)
S317-p

Chk1 (mouse)
S317-p

12302 : Phospho-Chk1 (Ser317) (D12H3) XP(R) Rabbit mAb

Effects on Modified Protein

enzymatic activity, induced
enzymatic activity, inhibited
molecular association, regulation
phosphorylation

Effects on Biological Processes:

DNA repair, induced
apoptosis, inhibited
carcinogenesis, induced
cell cycle regulation
signaling pathway regulation

Kinase, in vitro:

ATR (human)

Phosphatases, in vitro:

PPP2CA (human)

Putative in vivo kinases:

ATR (human)

Putative upstream phosphatases:

PPP2CA (human)

Regulatory protein:

ATM (human)
ATR (human)
BRCA1 (human)
BRCA2 (human)
CASP2 (human)
CDK1 (human)
CDK2 (human)
CDK5 (human)
CDT1 (human)
claspin (human)
CLK2 (human)
CtIP (human)
DKFZp686L1814 (human)
DMAP1 (human)
ETAA16 (human)
FAAP24 (human)
FAM44A (human)
FANCM (human)
FBXW7 (human)
FOXM1 (human)
Geminin (human)
LSD1 (human)
MCM7 (human)
NBS1 (human)
OBFC2B (human)
p16-INK4A (human)
p53 (human)
PERK (human)
POLH (human)
POLK (human)
PPP1CA (human)
PPP2CA (human)
SIRT6 (human)
TICRR (human)
TRAF4 (human)
USP28 (human)
USP29 (human)
WDHD1 (human)

Treatment

1NM-PP1
2-deoxyglucose
actinomycin_D
adriamycin
aphidicolin
ATM_inhibitor
AZ20
bleomycin
caffeine
camptothecin
carboplatin
CBP-93872
cell_adhesion
Ceralasertib
cisplatin
CPT
Et743
etoposide
fluorouracil
formaldehyde
fostriecin
gefitinib
Go_6976
hydroxyurea
ICRF-193
ionizing_radiation
KU-55933
lurbinectedin
MG132
mimosine
mitomycin_C
mitoxantrone
MK1775
MMS
nocodazole
okadaic_acid
olaparib
PD98059
PERK inhibitor 1
PF477736
PHA-767491
phorbol_ester
prexasertib
purvalanol
R03306
resveratrol
RO-3306
ryuvidine
SB203580
seliciclib
siRNA
SJG-136
SP600125
thapsigargin
thymidine
tioguanine
TMZ removal
topotecan
trichostatin_A
U0126
UCN-01
UV
UVC
VE-821
VRX0466617

Chk1 (human)	ENVkYsssQPEPRTG	S317-p
Chk1 (mouse)	ETVKFsSsQPEPRTG	S317-p
Chk1 (rat)	ENVKFSSSQPEPRTG	S317
Chk1 (chicken)	DKASYSTSQPEPGTG	S317

T330-p

TGLSLWDtsPsYIDK

Chk1 (human)	TGLSLWDtsPsYIDK	T330-p
Chk1 (mouse)	TGLSLWDTGPSNVDK	T330
Chk1 (rat)	TGLSLWDTGPSNVDK	T330
Chk1 (chicken)	TGGALWDSSTGSIDR	S330

S331-p

GLSLWDtsPsYIDKL

Upstream

Treatment

MG132
nocodazole
thymidine

Chk1 (human)	GLSLWDtsPsYIDKL	S331-p
Chk1 (mouse)	GLSLWDTGPSNVDKL	G331
Chk1 (rat)	GLSLWDTGPSNVDKL	G331
Chk1 (chicken)	GGALWDSSTGSIDRL	S331

S333-p

SLWDtsPsYIDKLVQ

Chk1 (human)	SLWDtsPsYIDKLVQ	S333-p
Chk1 (mouse)	SLWDTGPSNVDKLVQ	S333
Chk1 (rat)	SLWDTGPSNVDKLVQ	S333
Chk1 (chicken)	ALWDSSTGSIDRLVQ	G333

S343-p

DKLVQGIsFsQPtCP

Chk1 (human)	DKLVQGIsFsQPtCP	S343-p
Chk1 (mouse)	DKLVQGISFsQPTCP	S343
Chk1 (rat)	DKLVQGISFSQPTCP	S343
Chk1 (chicken)	DRLVQGISFsQPACP	S343

S345-p

LVQGIsFsQPtCPDH

Upstream

Downstream

188

Chk1 (human)
S345-p

Chk1 (mouse)
S345-p

Chk1 (rat)
S345-p

Effects on Modified Protein

enzymatic activity, induced
intracellular localization
molecular association, regulation
phosphorylation
protein degradation

Effects on Biological Processes:

DNA repair, induced
DNA repair, inhibited
apoptosis, altered
apoptosis, induced
apoptosis, inhibited
carcinogenesis, induced
carcinogenesis, inhibited
cell cycle regulation
cell growth, induced
cell growth, inhibited
signaling pathway regulation
transcription, induced

Kinase, in vitro:

ATR (human)
Cot (human)

Phosphatases, in vitro:

PPM1D (human)
PPP2CA (human)

Putative in vivo kinases:

ATM (human)
ATR (human)

Putative upstream phosphatases:

PPM1D (human)
PPP2CA (human)

Regulatory protein:

Akt1 (human)
AMBRA1 (human)
ATM (human)
ATR (human)
BAT1 (human)
CDC5L (human)
CDC6 (human)
CDCA2 (human)
CDK1 (human)
CDK2 (human)
CDT1 (human)
claspin (human)
CtIP (human)
DKFZp686L1814 (human)
DNAPK (human)
E4F1 (human)
EDEM1 (human)
EGFR (human)
ER-alpha (human)
FAM44A (human)
FANCD2 (human)
FBXO44 (human)
FOXM1 (human)
HDAC6 (human)
HER2 (human)
HRas (human)
HSP27 (human)
HSPA2 (human)
Ku70 (human)
MCM3 (human)
MCM7 (human)
MCPH1 (human)
N-Myc (human)
NBS1 (human)
NEIL1 (human)
NKX3-1 (human)
NONO (human)
OTUD6A (human)
p14ARF (human)
p16-INK4A (human)
p21Cip1 (human)
p53 (human)
PARG (human)
PCNT (human)
PERK (human)
POLK (human)
PPP1CA (human)
PPP2CA (human)
PPP2CB (human)
PPP4C (human)
PRPF19 (human)
PRPF3 (human)
PTEN (human)
RAD17 (human)
RNH1 (human)
RPA1 (human)
SAMHD1 (human)
SLFN11 (human)
SMEK2 (human)
SMG7 (human)
syndecan-4 (human)
TELO2 (human)
TICRR (human)
Tip60 (human)
TOPBP1 (human)
TRAF4 (human)
vpr p15 (retrovirus)
WDHD1 (human)

Treatment

11,11'-dideoxyverticillin
1NM-PP1
680C91
actinomycin_D
adavosertib
adriamycin
AG1478
alpelisib
aminothiazole_compound_25
AP21967
aphidicolin
AZ20
AZD1775
AZD5896
AZD6738
AZD7762
B02
Bersavine
beta-lapachone
BI-D1870
bleomycin
caffeine
camptothecin
carmustine
Caspase 9 inhibitor III
CBP-93872
cisplatin
compound 13
CPT
cycloheximide
doxycycline
DPN
EGF
Epo
estradiol
etoposide
eusynstyelamide B
Far-infrared
faslodex
fluorouracil
g_DNA
galiellalactone
gemcitabine
Go_6976
H2O2
hydroxyurea
hyperoxia
hypoxia
ICRF-193
IL-3
ionizing_radiation
ionomycin
ISRIB
KU-55933
kynurenine
lithium
lncRNAs
LY2606368
LY294002
M4344
MG132
mimosine
miRNA
mitomycin_C
MK-8776
MMS
NAC
nocodazole
NPB
nutlin-3
okadaic_acid
olaparib
PD173955
PD179483
PD98059
PDTC
peptide inhibitor
PERK inhibitor 1
PHA-767491
PPT
prexasertib
progesterone
psoralen
purvalanol
pyrvinium pamoate
rabusertib
resveratrol
ricolinostat
RO-3306
rucaparib
SB203580
SB216763
SB218078
seliciclib
serum
siRNA
SP600125
staurosporine
TAK-243
Talazoparib
taxol
testosterone
thapsigargin
thymidine
tivantinib
TMZ
TMZ removal
TNF
UCN-01
UV
UVC
VE-821
virus infection
vorinostat
VRX0466617
wortmannin

Chk1 (human)	LVQGIsFsQPtCPDH	S345-p
Chk1 (mouse)	LVQGISFsQPTCPEH	S345-p
Chk1 (rat)	LVQGISFSQPTCPDH	S345
Chk1 (chicken)	LVQGISFsQPACPEH	S345-p

T348-p

GIsFsQPtCPDHMLL

Chk1 (human)	GIsFsQPtCPDHMLL	T348-p
Chk1 (mouse)	GISFsQPTCPEHMLV	T348
Chk1 (rat)	GISFSQPTCPDHMLV	T348
Chk1 (chicken)	GISFsQPACPEHMLL	A348

S357-p

PDHMLLNsQLLGtPG

Chk1 (human)	PDHMLLNsQLLGtPG	S357-p
Chk1 (mouse)	PEHMLVNSQLLGTPG	S357
Chk1 (rat)	PDHMLVNSQLLGTPG	S357
Chk1 (chicken)	PEHMLLNSQLLGTPG	S357

T362-p

LNsQLLGtPGssQNP

Chk1 (human)	LNsQLLGtPGssQNP	T362-p
Chk1 (mouse)	VNSQLLGTPGSSQNP	T362
Chk1 (rat)	VNSQLLGTPGSSQNP	T362
Chk1 (chicken)	LNSQLLGTPGSSQSP	T362

S365-p

QLLGtPGssQNPWQR

Chk1 (human)	QLLGtPGssQNPWQR	S365-p
Chk1 (mouse)	QLLGTPGSSQNPWQR	S365
Chk1 (rat)	QLLGTPGSSQNPWQR	S365
Chk1 (chicken)	QLLGTPGSSQSPWQR	S365

S366-p

LLGtPGssQNPWQRL

Chk1 (human)	LLGtPGssQNPWQRL	S366-p
Chk1 (mouse)	LLGTPGSSQNPWQRL	S366
Chk1 (rat)	LLGTPGSSQNPWQRL	S366
Chk1 (chicken)	LLGTPGSSQSPWQRL	S366

T378-p

QRLVKRMtRFFtkLD

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
protein degradation

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

Chk1 (human)

Putative in vivo kinases:

Chk1 (human)

Treatment

AZD7762
etoposide
hydroxyurea
UCN-01

Chk1 (human)	QRLVKRMtRFFtkLD	T378-p
Chk1 (mouse)	QRLVKRMTRFFTKLD	T378
Chk1 (rat)	QRLVKRMTRFFTKLD	T378
Chk1 (chicken)	QRLVRRMTRFFTKLD	T378

T382-p

KRMtRFFtkLDADks

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
protein degradation

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

Chk1 (human)

Putative in vivo kinases:

Chk1 (human)

Treatment

AZD7762
etoposide
hydroxyurea
UCN-01

Chk1 (human)	KRMtRFFtkLDADks	T382-p
Chk1 (mouse)	KRMTRFFTKLDADKS	T382
Chk1 (rat)	KRMTRFFTKLDADKS	T382
Chk1 (chicken)	RRMTRFFTKLDADGS	T382

K383-ub

RMtRFFtkLDADksY

Chk1 (human)	RMtRFFtkLDADksY	K383-ub
Chk1 (mouse)	RMTRFFTKLDADKSY	K383
Chk1 (rat)	RMTRFFTKLDADKSY	K383
Chk1 (chicken)	RMTRFFTKLDADGSY	K383

K388-ub

FtkLDADksYQCLKE

Chk1 (human)	FtkLDADksYQCLKE	K388-ub
Chk1 (mouse)	FTKLDADKSYQCLKE	K388
Chk1 (rat)	FTKLDADKSYQCLKE	K388
Chk1 (chicken)	FTKLDADGSYRSLRD	G388

S389-p

tkLDADksYQCLKET

Chk1 (human)	tkLDADksYQCLKET	S389-p
Chk1 (mouse)	TKLDADKSYQCLKET	S389
Chk1 (rat)	TKLDADKSYQCLKET	S389
Chk1 (chicken)	TKLDADGSYRSLRDV	S389

K399-ub

CLKETCEkLGYQWKK

Chk1 (human)	CLKETCEkLGYQWKK	K399-ub
Chk1 (mouse)	CLKETFEKLGYQWKK	K399
Chk1 (rat)	CLKETFEKLGYQWKK	K399
Chk1 (chicken)	SLRDVCEKMGYGWKQ	K399

S407-p

LGYQWKKsCMNQVTI

Chk1 (human)	LGYQWKKsCMNQVTI	S407-p
Chk1 (mouse)	LGYQWKKSCMNQVTV	S407
Chk1 (rat)	LGYQWKKSCMNQVTV	S407
Chk1 (chicken)	MGYGWKQSCTNQVTI	S407

K423-ub

TTDRRNNkLIFKVNL

Chk1 (human)	TTDRRNNkLIFKVNL	K423-ub
Chk1 (mouse)	TTDRRNNKLIFKINL	K423
Chk1 (rat)	TMDRRNNKLIFKINL	K423
Chk1 (chicken)	TTDRRNNKLIFKVNL	K423

K436-ub

NLLEMDDkILVDFRL

Upstream

Downstream

Effects on Modified Protein

protein degradation

Effects on Biological Processes:

apoptosis, induced
carcinogenesis, inhibited
cell cycle regulation

Treatment

siRNA

Chk1 (human)	NLLEMDDkILVDFRL	K436-ub
Chk1 (mouse)	NLVEMDEKILVDFRL	K436
Chk1 (rat)	NLVEMDEKILVDFRL	K436
Chk1 (chicken)	NLLEMESRILVDFRL	R436

K445-ub

LVDFRLSkGDGLEFk

Chk1 (human)	LVDFRLSkGDGLEFk	K445-ub
Chk1 (mouse)	LVDFRLSKGDGLEFK	K445
Chk1 (rat)	LVDFRLSKGDGLEFK	K445
Chk1 (chicken)	LVDFRLSKGDGLEFK	K445

K452-ac

kGDGLEFkRHFLKIK

Chk1 (human)	kGDGLEFkRHFLKIK	K452-ac
Chk1 (mouse)	KGDGLEFKRHFLKIK	K452
Chk1 (rat)	KGDGLEFKRHFLKIK	K452
Chk1 (chicken)	KGDGLEFKRHFLKIK	K452

K452-ub

kGDGLEFkRHFLKIK

Chk1 (human)	kGDGLEFkRHFLKIK	K452-ub
Chk1 (mouse)	KGDGLEFKRHFLKIK	K452
Chk1 (rat)	KGDGLEFKRHFLKIK	K452
Chk1 (chicken)	KGDGLEFKRHFLKIK	K452

K461-ub

HFLKIKGkLIDIVss

Chk1 (human)	HFLKIKGkLIDIVss	K461-ub
Chk1 (mouse)	HFLKIKGKLsDVVSS	K461
Chk1 (rat)	HFLKIKGKLSDIVSS	K461
Chk1 (chicken)	HFLKIKGKLSDVVST	K461

I463

LKIKGkLIDIVssQK

Chk1 (human)	LKIKGkLIDIVssQK	I463
Chk1 (mouse)	LKIKGKLsDVVSSQK	S463-p
Chk1 (rat)	LKIKGKLSDIVSSQK	S463
Chk1 (chicken)	LKIKGKLSDVVSTQK	S463

S467-p

GkLIDIVssQKIWLP

Chk1 (human)	GkLIDIVssQKIWLP	S467-p
Chk1 (mouse)	GKLsDVVSSQKVWFP	S467
Chk1 (rat)	GKLSDIVSSQKVWFP	S467
Chk1 (chicken)	GKLSDVVSTQKVWLP	S467

S468-p

kLIDIVssQKIWLPA

Chk1 (human)	kLIDIVssQKIWLPA	S468-p
Chk1 (mouse)	KLsDVVSSQKVWFPV	S468
Chk1 (rat)	KLSDIVSSQKVWFPV	S468
Chk1 (chicken)	KLSDVVSTQKVWLPP	T468