ASK1 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

gap

ASK1 (human)	gap	-
ASK1 iso5 (human)	CLARRARssPELWLA	S55-p
ASK1 (mouse)	gap	-
ASK1 (rat)	gap	-

gap

ASK1 (human)	gap	-
ASK1 iso5 (human)	LARRARssPELWLAR	S56-p
ASK1 (mouse)	gap	-
ASK1 (rat)	gap	-

S82-p

SATRGRGssVGGGSr

ASK1 (human)	SATRGRGssVGGGSr	S82-p
ASK1 iso5 (human)	SATRGRGSSVGGGSR	S162
ASK1 (mouse)	SATRGRGNsGSGGGR	N89
ASK1 (rat)	SATRGRGNSGSGGGR	N89

S83-p

ATRGRGssVGGGSrR

Upstream

Downstream

ASK1 (human)
S83-p

3761 : Phospho-ASK1 (Ser83) Antibody -- discontinued

Effects on Modified Protein

enzymatic activity, induced
enzymatic activity, inhibited
phosphorylation

Effects on Biological Processes:

apoptosis, altered
apoptosis, inhibited
carcinogenesis, induced
signaling pathway regulation
transcription, altered

Kinase, in vitro:

Akt1 (human)
Akt2 (human)
Pim1 (human)
RSK2 (human)

Putative in vivo kinases:

Akt1 (human)
Akt2 (human)
Pim1 (human)
RSK2 (human)

Regulatory protein:

Akt1 (human)
HSP90A (human)
LKB1 (human)
PDK1 (human)

Treatment

benzodiazepinones
culturing_of_cells
DPI
estradiol
faslodex
fluoromethylketone
H2O2
LY294002
nocodazole
siRNA
tanespimycin
wortmannin

ASK1 (human)	ATRGRGssVGGGSrR	S83-p
ASK1 iso5 (human)	ATRGRGSSVGGGSRR	S163
ASK1 (mouse)	ATRGRGNsGSGGGRR	S90-p
ASK1 (rat)	ATRGRGNSGSGGGRR	S90

R89-me

ssVGGGSrRTTVAYV

Downstream

Effects on Modified Protein

molecular association, regulation
phosphorylation

ASK1 (human)	ssVGGGSrRTTVAYV	R89-me
ASK1 iso5 (human)	SSVGGGSRRTTVAYV	R169
ASK1 (mouse)	NsGSGGGRRTTVAYV	R96
ASK1 (rat)	NSGSGGGRRTTVAYV	R96

S101-p

AYVINEAsQGQLVVA

ASK1 (human)	AYVINEAsQGQLVVA	S101-p
ASK1 iso5 (human)	AYVINEASQGQLVVA	S181
ASK1 (mouse)	AYVINEASQGQLVVA	S108
ASK1 (rat)	AYVINEASQGQLVVA	S108

S425

WFKKAFESEPTLQSG

ASK1 (human)	WFKKAFESEPTLQSG	S425
ASK1 iso5 (human)	WFKKAFESEPTLQSG	S505
ASK1 (mouse)	WFKKAFEsEPTLQsG	S432-p
ASK1 (rat)	WFKKAFESEPTLQSG	S432

S431

ESEPTLQSGINYAVL

ASK1 (human)	ESEPTLQSGINYAVL	S431
ASK1 iso5 (human)	ESEPTLQSGINYAVL	S511
ASK1 (mouse)	EsEPTLQsGINYAVL	S438-p
ASK1 (rat)	ESEPTLQSGINYAVL	S438

S461-p

KVGVKLSsLLGkKGN

ASK1 (human)	KVGVKLSsLLGkKGN	S461-p
ASK1 iso5 (human)	KVGVKLSSLLGKKGN	S541
ASK1 (mouse)	KVGVKLSSLLGKKGN	S468
ASK1 (rat)	KVGVKLSSLLGKKGN	S468

K465-ub

KLSsLLGkKGNLEKL

ASK1 (human)	KLSsLLGkKGNLEKL	K465-ub
ASK1 iso5 (human)	KLSSLLGKKGNLEKL	K545
ASK1 (mouse)	KLSSLLGKKGNLEKL	K472
ASK1 (rat)	KLSSLLGKKGNLEKL	K472

T528-p

YKHFVKLtTEQPVAk

ASK1 (human)	YKHFVKLtTEQPVAk	T528-p
ASK1 iso5 (human)	YKHFVKLTTEQPVAK	T608
ASK1 (mouse)	YKHFVKLTTEQPSAK	T535
ASK1 (rat)	YKHFVKLTTEQPTAK	T535

K535-sm

tTEQPVAkQELVDFW

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

apoptosis, inhibited

ASK1 (human)	tTEQPVAkQELVDFW	K535-sm
ASK1 iso5 (human)	TTEQPVAKQELVDFW	K615
ASK1 (mouse)	TTEQPSAKQELVDFW	K542
ASK1 (rat)	TTEQPTAKQELVDFW	K542

Y570-p

ILEPTKIyQPSyLSI

ASK1 (human)	ILEPTKIyQPSyLSI	Y570-p
ASK1 iso5 (human)	ILEPTKIYQPSYLSI	Y650
ASK1 (mouse)	ILEPTKIYQPSYLSI	Y577
ASK1 (rat)	ILEPTKIYQPSYLSI	Y577

Y574-p

TKIyQPSyLSINNEV

ASK1 (human)	TKIyQPSyLSINNEV	Y574-p
ASK1 iso5 (human)	TKIYQPSYLSINNEV	Y654
ASK1 (mouse)	TKIYQPSYLSINNEV	Y581
ASK1 (rat)	TKIYQPSYLSINNEV	Y581

Y691

VVLGKGTYGIVYAGR

ASK1 (human)	VVLGKGTYGIVYAGR	Y691
ASK1 iso5 (human)	VVLGKGTYGIVYAGR	Y771
ASK1 (mouse)	VVLGKGTYGIVYAGR	Y698
ASK1 (rat)	VVLGKGTyGIVYAGR	Y697-p

Y718-p

IPERDSRySQPLHEE

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein degradation

Kinase, in vitro:

JAK1 (human)
JAK2 (human)

Putative in vivo kinases:

JAK2 (human)

Putative upstream phosphatases:

SHP-2 (human)

Regulatory protein:

SOCS1 (human)

Treatment

IFN-beta
IFN-gamma
TNF

ASK1 (human)	IPERDSRySQPLHEE	Y718-p
ASK1 iso5 (human)	IPERDSRYSQPLHEE	Y798
ASK1 (mouse)	IPERDSRYSQPLHEE	Y725
ASK1 (rat)	IPERDSRYSQPLHEE	Y724

K730-ub

HEEIALHkHLKHKNI

ASK1 (human)	HEEIALHkHLKHKNI	K730-ub
ASK1 iso5 (human)	HEEIALHKHLKHKNI	K810
ASK1 (mouse)	HEEIALHKHLKHKNI	K737
ASK1 (rat)	HEEIALHKHLKHKNI	K736

K785-ub

QTIGFYTkQILEGLK

ASK1 (human)	QTIGFYTkQILEGLK	K785-ub
ASK1 iso5 (human)	QTIGFYTKQILEGLK	K865
ASK1 (mouse)	QTIGFYTKQILEGLK	K792
ASK1 (rat)	QTIGFYTKQILEGLK	K791

T813-p

GDNVLINtySGVLKI

Upstream

Downstream

Effects on Biological Processes:

transcription, altered

Kinase, in vitro:

ASK1 (human)

ASK1 (human)	GDNVLINtySGVLKI	T813-p
ASK1 iso5 (human)	GDNVLINTYSGVLKI	T893
ASK1 (mouse)	GDNVLINTYSGVLKI	T820
ASK1 (rat)	GDNVLINTYSGVLKI	T819

Y814-p

DNVLINtySGVLKIs

ASK1 (human)	DNVLINtySGVLKIs	Y814-p
ASK1 iso5 (human)	DNVLINTYSGVLKIS	Y894
ASK1 (mouse)	DNVLINTYSGVLKIS	Y821
ASK1 (rat)	DNVLINTYSGVLKIS	Y820

S821-p

ySGVLKIsDFGtSKR

ASK1 (human)	ySGVLKIsDFGtSKR	S821-p
ASK1 iso5 (human)	YSGVLKISDFGTSKR	S901
ASK1 (mouse)	YSGVLKISDFGTSKR	S828
ASK1 (rat)	YSGVLKISDFGTSKR	S827

T825-p

LKIsDFGtSKRLAGI

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
protein conformation

Effects on Biological Processes:

apoptosis, induced
transcription, induced

Kinase, in vitro:

LRRK2 (human)

Putative in vivo kinases:

LRRK2 (human)

Treatment

LRRK2-IN-1
oxidative_stress

ASK1 (human)	LKIsDFGtSKRLAGI	T825-p
ASK1 iso5 (human)	LKISDFGTSKRLAGI	T905
ASK1 (mouse)	LKISDFGTSKRLAGI	T832
ASK1 (rat)	LKISDFGTSKRLAGI	T831

T836

LAGINPCTEtFTGtL

ASK1 (human)	LAGINPCTEtFTGtL	T836
ASK1 iso5 (human)	LAGINPCTETFTGTL	T916
ASK1 (mouse)	LAGINPCtEtFTGtL	T843-p
ASK1 (rat)	LAGINPCTETFTGTL	T842

T838-p

GINPCTEtFTGtLQY

Upstream

Downstream

ASK1 (human)
T838-p

3765 : Phospho-ASK1 (Thr845) Antibody

ASK1 (mouse)
T845-p

3765 : Phospho-ASK1 (Thr845) Antibody

Effects on Modified Protein

activity, induced
enzymatic activity, induced
molecular association, regulation
phosphorylation

Effects on Biological Processes:

apoptosis, altered
apoptosis, induced
carcinogenesis, induced
cell adhesion, altered
cell cycle regulation
cell growth, induced
transcription, altered
translation, induced

Kinase, in vitro:

ASK1 (human)

Phosphatases, in vitro:

CDC25C (human)

Putative in vivo kinases:

LRRK2 (human)
PRKD1 (human)

Putative upstream phosphatases:

CDC25C (human)
PPP5C (human)

Regulatory protein:

ASK1 (human)
HSP90A (human)
MEKK6 (human)
PDK1 (human)
PGAM5 (human)
RIPK3 (human)
RSK2 (human)
SHP-2 (human)

Treatment

GSK1016790A
4-hydroxy-2-nonenal
benzodiazepinones
DDO3704
DDO3705
DDO3706
DDO3707
DDO3708
DDO3709
DDO3709R8
DDO3710
DDO3711
DDO3712
DDO3713
DDO3714
dolastatin_10
ethanol
EUK8
Go_6976
Go_6983
H2O2
IOA
LRRK2-IN-1
LY294002
monastrol
NAC
nocodazole
oxidative_stress
P5SA2
siRNA
tanespimycin
taxol
TCASK10
TNF

ASK1 (human)	GINPCTEtFTGtLQY	T838-p
ASK1 iso5 (human)	GINPCTETFTGTLQY	T918
ASK1 (mouse)	GINPCtEtFTGtLQY	T845-p
ASK1 (rat)	GINPCTETFTGTLQY	T844

T842-p

CTEtFTGtLQYMAPE

Upstream

Downstream

Effects on Biological Processes:

transcription, altered

Kinase, in vitro:

ASK1 (human)

ASK1 (human)	CTEtFTGtLQYMAPE	T842-p
ASK1 iso5 (human)	CTETFTGTLQYMAPE	T922
ASK1 (mouse)	CtEtFTGtLQYMAPE	T849-p
ASK1 (rat)	CTETFTGTLQYMAPE	T848

K946-ac

KVSSKKKkTQPKLSA

ASK1 (human)	KVSSKKKkTQPKLSA	K946-ac
ASK1 iso5 (human)	KVSSKKKKTQPKLSA	K1026
ASK1 (mouse)	KVSSKKKKTQPKLSA	K953
ASK1 (rat)	KVSSKKKKTQPKLSA	K952

A956

PKLSALSAGsNEYLR

ASK1 (human)	PKLSALSAGsNEYLR	A956
ASK1 iso5 (human)	PKLSALSAGSNEYLR	A1036
ASK1 (mouse)	PKLSALStGsNEYLR	T963-p
ASK1 (rat)	PKLSALMTDSNQYLR	T962

S958-p

LSALSAGsNEYLRsI

ASK1 (human)	LSALSAGsNEYLRsI	S958-p
ASK1 iso5 (human)	LSALSAGSNEYLRSI	S1038
ASK1 (mouse)	LSALStGsNEYLRSI	S965-p
ASK1 (rat)	LSALMTDSNQYLRSI	S964

S964-p

GsNEYLRsIsLPVPV

ASK1 (human)	GsNEYLRsIsLPVPV	S964-p
ASK1 iso5 (human)	GSNEYLRSISLPVPV	S1044
ASK1 (mouse)	GsNEYLRSIsLPVPV	S971
ASK1 (rat)	DSNQYLRSISLPVPV	S970

S966-p

NEYLRsIsLPVPVLV

Upstream

Downstream

ASK1 (human)
S966-p

3764 : Phospho-ASK1 (Ser967) Antibody

ASK1 (mouse)
S973-p

3764 : Phospho-ASK1 (Ser967) Antibody

Effects on Modified Protein

activity, inhibited
enzymatic activity, inhibited
molecular association, regulation

Effects on Biological Processes:

apoptosis, altered
apoptosis, induced
apoptosis, inhibited
signaling pathway regulation
transcription, altered

Kinase, in vitro:

ASK1 (human)

Phosphatases, in vitro:

PPP3CA (human)

Putative in vivo kinases:

PDK1 (human)

Putative upstream phosphatases:

PPP3CA (human)

Regulatory protein:

LKB1 (human)
PDK1 (human)
RSK2 (human)

Treatment

angiotensin_2
benzodiazepinones
beta-amyloid_42
calyculin_A
ciclosporin
EUK8
Go_6976
Go_6983
H2O2
IOA
ionomycin
isoproterenol
NAC
nocodazole
okadaic_acid
phenylephrine
siRNA
thapsigargin

ASK1 (human)	NEYLRsIsLPVPVLV	S966-p
ASK1 iso5 (human)	NEYLRSISLPVPVLV	S1046
ASK1 (mouse)	NEYLRSIsLPVPVLV	S973-p
ASK1 (rat)	NQYLRSISLPVPVLV	S972

Y982-p

DTSSSSEyGSVsPDT

ASK1 (human)	DTSSSSEyGSVsPDT	Y982-p
ASK1 iso5 (human)	DTSSSSEYGSVSPDD	Y1062
ASK1 (mouse)	DTSSSSEYGSVsPDT	Y989
ASK1 (rat)	DTSSSSEYGSVsPDT	Y988

S986-p

SSEyGSVsPDTELKV

ASK1 (human)	SSEyGSVsPDTELKV	S986-p
ASK1 iso5 (human)	SSEYGSVSPDDTELK	S1066
ASK1 (mouse)	SSEYGSVsPDTELKA	S993-p
ASK1 (rat)	SSEYGSVsPDTELKA	S992-p

S997-p

ELKVDPFsFKTRAKS

ASK1 (human)	ELKVDPFsFKTRAKS	S997-p
ASK1 iso5 (human)	ELKVDPFSFKTRAKS	S1078
ASK1 (mouse)	ELKADPFSFKARAKS	S1004
ASK1 (rat)	ELKADPFSFKTRAKS	S1003

S1029-p

DENFEDHsAPPsPEE

Upstream

Treatment

ischemia

ASK1 (human)	DENFEDHsAPPsPEE	S1029-p
ASK1 iso5 (human)	DENFEDHSAPPSPEE	S1110
ASK1 (mouse)	DENFEDHsAPPsPEE	S1036-p
ASK1 (rat)	DENFEDHsAPPsPEE	S1035-p

S1033-p

EDHsAPPsPEEKDSG

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited
phosphorylation

Effects on Biological Processes:

apoptosis, altered

Regulatory protein:

RIPK3 (human)

Treatment

ethanol
ischemia

ASK1 (human)	EDHsAPPsPEEKDSG	S1033-p
ASK1 iso5 (human)	EDHSAPPSPEEKDSG	S1114
ASK1 (mouse)	EDHsAPPsPEEKDSG	S1040-p
ASK1 (rat)	EDHsAPPsPEEKDSG	S1039-p

T1059-p

ATLHRILtEDQDkIV

ASK1 (human)	ATLHRILtEDQDkIV	T1059-p
ASK1 iso5 (human)	ATLHRILTEDQDKIV	T1140
ASK1 (mouse)	ATLHRILtEDQDKVV	T1066-p
ASK1 (rat)	ATLHRILTEDQDKVV	T1065

K1064-ub

ILtEDQDkIVRNLME

ASK1 (human)	ILtEDQDkIVRNLME	K1064-ub
ASK1 iso5 (human)	ILTEDQDKIVRNLME	K1145
ASK1 (mouse)	ILtEDQDKVVRNLME	K1071
ASK1 (rat)	ILTEDQDKVVRNLME	K1070

S1072

IVRNLMESLAQGAEE

ASK1 (human)	IVRNLMESLAQGAEE	S1072
ASK1 iso5 (human)	IVRNLMESLAQGAEE	S1153
ASK1 (mouse)	VVRNLMEsLAQGAEE	S1079-p
ASK1 (rat)	VVRNLMESLAQGPEE	S1078

K1083-sm

GAEEPKLkWEHITTL

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

apoptosis, inhibited

ASK1 (human)	GAEEPKLkWEHITTL	K1083-sm
ASK1 iso5 (human)	GAEEPKLKWEHITTL	K1164
ASK1 (mouse)	GAEEPKLKWEHITTL	K1090
ASK1 (rat)	GPEEPKLKWEHITTL	K1089

T1109-p

DRKIIATtLSKLkLE

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited

Effects on Biological Processes:

carcinogenesis, induced

Kinase, in vitro:

RSK2 (human)

Putative in vivo kinases:

RSK2 (human)

Treatment

fluoromethylketone
siRNA

ASK1 (human)	DRKIIATtLSKLkLE	T1109-p
ASK1 iso5 (human)	DRKIIATTLSKLKLE	T1190
ASK1 (mouse)	DRKIIATTLSKLKLE	T1116
ASK1 (rat)	DRKIIATTLSRLKLE	T1115

K1114-sm

ATtLSKLkLELDFDS

ASK1 (human)	ATtLSKLkLELDFDS	K1114-sm
ASK1 iso5 (human)	ATTLSKLKLELDFDS	K1195
ASK1 (mouse)	ATTLSKLKLELDFDS	K1121
ASK1 (rat)	ATTLSRLKLELDFDS	K1120

S1240-p

DSQSAHRsLNVQLGR

ASK1 (human)	DSQSAHRsLNVQLGR	S1240-p
ASK1 iso5 (human)	DSQSAHRSLNVQLGR	S1321
ASK1 (mouse)	DSQNAHRSLNVQLGR	S1247
ASK1 (rat)	DSQNAHRSLNVQLGR	S1246

S1303

LPVFHLNSSGTNTED

ASK1 (human)	LPVFHLNSSGTNTED	S1303
ASK1 iso5 (human)	LPVFHLNSSGTNTED	S1384
ASK1 (mouse)	FPVCHLNsPGTTTED	S1310-p
ASK1 (rat)	FPVCHLNAPGTAIED	A1309

T1326-p

VNGADEDtISRFLAE

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, inhibited

Effects on Biological Processes:

carcinogenesis, induced

Kinase, in vitro:

RSK2 (human)

Putative in vivo kinases:

RSK2 (human)

Treatment

fluoromethylketone
siRNA

ASK1 (human)	VNGADEDtISRFLAE	T1326-p
ASK1 iso5 (human)	VNGADEDTISRFLAE	T1407
ASK1 (mouse)	ENGADEDTISRFLAE	T1333
ASK1 (rat)	ENGADEDTISRFLAE	T1332