SMAD2 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

S2-p

______MsSILPFtP

Upstream

Treatment

nocodazole

SMAD2 (human)	______MsSILPFtP	S2-p
SMAD2 (mouse)	______MSSILPFtP	S2
SMAD2 (rat)	______MSSILPFtP	S2
SMAD2 (cow)	______MSSILPFTP	S2

T8-p

MsSILPFtPPVVkRL

Upstream

Downstream

Effects on Modified Protein

activity, induced
molecular association, regulation
protein stabilization

Effects on Biological Processes:

transcription, induced

Kinase, in vitro:

ERK1 (human)

Putative in vivo kinases:

ERK1 (human)

Treatment

EGF
ischemia
LRRK2-IN-1
nocodazole
U0126

SMAD2 (human)	MsSILPFtPPVVkRL	T8-p
SMAD2 (mouse)	MSSILPFtPPVVKRL	T8-p
SMAD2 (rat)	MSSILPFtPPVVKRL	T8-p
SMAD2 (cow)	MSSILPFTPPVVKRL	T8

K13-ub

PFtPPVVkRLLGWkk

SMAD2 (human)	PFtPPVVkRLLGWkk	K13-ub
SMAD2 (mouse)	PFtPPVVKRLLGWKK	K13
SMAD2 (rat)	PFtPPVVKRLLGWKK	K13
SMAD2 (cow)	PFTPPVVKRLLGWKK	K13

K19-ac

VkRLLGWkksAGGSG

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation
protein conformation

Effects on Biological Processes:

cell cycle regulation
cell growth, inhibited
signaling pathway regulation
transcription, induced

Treatment

anacardic acid
TGF-beta
trichostatin_A

SMAD2 (human)	VkRLLGWkksAGGSG	K19-ac
SMAD2 (mouse)	VKRLLGWKKSAGGSG	K19
SMAD2 (rat)	VKRLLGWKKSAGGSG	K19
SMAD2 (cow)	VKRLLGWKKSAGGSG	K19

K20-ac

kRLLGWkksAGGSGG

Upstream

Downstream

Effects on Modified Protein

intracellular localization

Effects on Biological Processes:

cell cycle regulation
cell growth, inhibited
signaling pathway regulation
transcription, induced

Treatment

TGF-beta
trichostatin_A

SMAD2 (human)	kRLLGWkksAGGSGG	K20-ac
SMAD2 (mouse)	KRLLGWKKSAGGSGG	K20
SMAD2 (rat)	KRLLGWKKSAGGSGG	K20
SMAD2 (cow)	KRLLGWKKSAGGSGG	K20

S21-p

RLLGWkksAGGSGGA

SMAD2 (human)	RLLGWkksAGGSGGA	S21-p
SMAD2 (mouse)	RLLGWKKSAGGSGGA	S21
SMAD2 (rat)	RLLGWKKSAGGSGGA	S21
SMAD2 (cow)	RLLGWKKSAGGSGGA	S21

K39-ac

EQNGQEEkWCEKAVK

Upstream

Downstream

Effects on Modified Protein

intracellular localization

Effects on Biological Processes:

cell cycle regulation
cell growth, inhibited
signaling pathway regulation
transcription, induced

Treatment

TGF-beta
trichostatin_A

SMAD2 (human)	EQNGQEEkWCEKAVK	K39-ac
SMAD2 (mouse)	EQNGQEEKWCEKAVK	K39
SMAD2 (rat)	EQNGQEEKWCEKAVK	K39
SMAD2 (cow)	EQNGQEEKWCEKAVK	K39

K63-ub

GRLDELEkAITTQNC

SMAD2 (human)	GRLDELEkAITTQNC	K63-ub
SMAD2 (mouse)	GRLDELEKAITTQNC	K63
SMAD2 (rat)	GRLDELEKAITTQNC	K63
SMAD2 (cow)	GRLDELEKAITTQNC	K63

Y102-p

QWDTTGLySFSEQTR

SMAD2 (human)	QWDTTGLySFSEQTR	Y102-p
SMAD2 (mouse)	QWDTTGLySFSEQTR	Y102-p
SMAD2 (rat)	QWDTTGLYSFSEQTR	Y102
SMAD2 (cow)	QWDTTGLYSFSEQTR	Y102

S110-p

SFSEQTRsLDGRLQV

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation

Kinase, in vitro:

CAMK2A (human)

Putative in vivo kinases:

CAMK2A (human)

SMAD2 (human)	SFSEQTRsLDGRLQV	S110-p
SMAD2 (mouse)	SFSEQTRSLDGRLQV	S110
SMAD2 (rat)	SFSEQTRSLDGRLQV	S110
SMAD2 (cow)	SFSEQTRSLDGRLQV	S110

K156-sm

CEYAFNLkkDEVCVN

SMAD2 (human)	CEYAFNLkkDEVCVN	K156-sm
SMAD2 (mouse)	CEYAFNLKKDEVCVN	K156
SMAD2 (rat)	CEYAFSLKKDEVCVN	K156
SMAD2 (cow)	CEYAFNLKKDEVCVN	K156

K156-ub

CEYAFNLkkDEVCVN

SMAD2 (human)	CEYAFNLkkDEVCVN	K156-ub
SMAD2 (mouse)	CEYAFNLKKDEVCVN	K156
SMAD2 (rat)	CEYAFSLKKDEVCVN	K156
SMAD2 (cow)	CEYAFNLKKDEVCVN	K156

K157-ub

EYAFNLkkDEVCVNP

SMAD2 (human)	EYAFNLkkDEVCVNP	K157-ub
SMAD2 (mouse)	EYAFNLKKDEVCVNP	K157
SMAD2 (rat)	EYAFSLKKDEVCVNP	K157
SMAD2 (cow)	EYAFNLKKDEVCVNP	K157

T172-p

YHYQRVEtPVLPPVL

SMAD2 (human)	YHYQRVEtPVLPPVL	T172-p
SMAD2 (mouse)	YHYQRVEtPVLPPVL	T172-p
SMAD2 (rat)	YHYQRVETPVLPPVL	T172
SMAD2 (cow)	YHYQRVETPVLPPVL	T172

T197-p

LPPLDDYtHSIPENT

Upstream

Kinase, in vitro:

GRK2 (human)

SMAD2 (human)	LPPLDDYtHSIPENT	T197-p
SMAD2 (mouse)	LPPLDDYTHSIPENT	T197
SMAD2 (rat)	LPPLDDYTHSIPENT	T197
SMAD2 (cow)	LPPLDDYTHSIPENT	T197

T220-p

QSNYIPEtPPPGYIS

Upstream

Downstream

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
protein stabilization

Effects on Biological Processes:

carcinogenesis, induced
signaling pathway regulation
transcription, induced

Kinase, in vitro:

ERK1 (human)
ERK2 (human)
OSR1 (human)

Putative in vivo kinases:

ERK1 (human)
OSR1 (human)

Regulatory protein:

FOXO4 (human)
HRas (human)
MEK1 (human)
NEDD4L (human)
TGFB1 (human)

Treatment

alvocidib
BMP2
EGF
MG132
PD98059
SB203580
siRNA
TGF-beta
wortmannin
Y27632

SMAD2 (human)	QSNYIPEtPPPGYIS	T220-p
SMAD2 (mouse)	QSNYIPETPPPGYIS	T220
SMAD2 (rat)	QSNYIPETPPPGYIS	T220
SMAD2 (cow)	QSNYIPEtPPPGYIS	T220-p

S240-p

SDQQLNQsMDTGsPA

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation

Kinase, in vitro:

CAMK2A (human)

Putative in vivo kinases:

CAMK2A (human)

Treatment

decorin
EGF
KN-93
TGF-beta

SMAD2 (human)	SDQQLNQsMDTGsPA	S240-p
SMAD2 (mouse)	SDQQLNQSMDTGsPA	S240
SMAD2 (rat)	SDQQLNQSMDTGSPA	S240
SMAD2 (cow)	SDQQLNQSMDTGsPA	S240

S245-p

NQsMDTGsPAELsPT

Upstream

Downstream

SMAD2 (human)
S245-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

SMAD2 (mouse)
S245-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

SMAD2 (rat)
S245-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
protein conformation
protein stabilization

Effects on Biological Processes:

cell motility, altered
transcription, induced

Kinase, in vitro:

ERK1 (human)
ERK2 (human)
MELK (mouse)

Putative in vivo kinases:

ERK1 (human)
MELK (mouse)

Regulatory protein:

HRas (human)
MEK1 (human)

Treatment

alvocidib
EGF
LY2109761
LY294002
PD98059
SB202190
SB431542
TGF-beta
U0126
wortmannin

SMAD2 (human)	NQsMDTGsPAELsPT	S245-p
SMAD2 (mouse)	NQSMDTGsPAELSPT	S245-p
SMAD2 (rat)	NQSMDTGSPAELSPT	S245
SMAD2 (cow)	NQSMDTGsPAELsPT	S245-p

S250-p

TGsPAELsPTTLsPV

Upstream

Downstream

SMAD2 (human)
S250-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

SMAD2 (mouse)
S250-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

SMAD2 (rat)
S250-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
protein conformation
protein stabilization

Effects on Biological Processes:

cell motility, altered
transcription, induced

Kinase, in vitro:

ERK1 (human)
ERK2 (human)

Putative in vivo kinases:

ERK1 (human)

Regulatory protein:

HRas (human)
MEK1 (human)

Treatment

EGF
LY2109761
PD98059
TGF-beta
U0126
wortmannin

SMAD2 (human)	TGsPAELsPTTLsPV	S250-p
SMAD2 (mouse)	TGsPAELSPTTLSPV	S250
SMAD2 (rat)	TGSPAELSPTTLSPV	S250
SMAD2 (cow)	TGsPAELsPTTLsPV	S250-p

S255-p

ELsPTTLsPVNHsLD

Upstream

Downstream

SMAD2 (human)
S255-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

SMAD2 (mouse)
S255-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

SMAD2 (rat)
S255-p

3104 : Phospho-Smad2 (Ser245/250/255) Antibody

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
protein conformation
protein stabilization

Effects on Biological Processes:

cell motility, altered
transcription, induced

Kinase, in vitro:

ERK1 (human)
ERK2 (human)

Putative in vivo kinases:

ERK1 (human)

Regulatory protein:

HRas (human)
MEK1 (human)

Treatment

alvocidib
EGF
LY2109761
LY294002
PD98059
SB202190
TGF-beta
U0126
wortmannin

SMAD2 (human)	ELsPTTLsPVNHsLD	S255-p
SMAD2 (mouse)	ELSPTTLSPVNHSLD	S255
SMAD2 (rat)	ELSPTTLSPVNHSLD	S255
SMAD2 (cow)	ELsPTTLsPVNHSLD	S255-p

S260-p

TLsPVNHsLDLQPVT

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation

Kinase, in vitro:

CAMK2A (human)

Putative in vivo kinases:

CAMK2A (human)

SMAD2 (human)	TLsPVNHsLDLQPVT	S260-p
SMAD2 (mouse)	TLSPVNHSLDLQPVT	S260
SMAD2 (rat)	TLSPVNHSLDLQPVT	S260
SMAD2 (cow)	TLsPVNHSLDLQPVT	S260

T324-p

SNVNRNAtVEMTRRH

SMAD2 (human)	SNVNRNAtVEMTRRH	T324-p
SMAD2 (mouse)	SNVNRNAtVEMTRRH	T324-p
SMAD2 (rat)	SNVNRNATVEMTRRH	T324
SMAD2 (cow)	SNVNRNATVEMTRRH	T324

S417-p

RMCTIRMsFVkGWGA

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation

Effects on Biological Processes:

signaling pathway regulation
transcription, inhibited

Kinase, in vitro:

PAK2 (human)

Putative in vivo kinases:

PAK2 (human)

Treatment

IPA-3

SMAD2 (human)	RMCTIRMsFVkGWGA	S417-p
SMAD2 (mouse)	RMCTIRMSFVKGWGA	S417
SMAD2 (rat)	RMCTIRMSFVKGWGA	S417
SMAD2 (cow)	RMCTIRMSFVKGWGA	S417

K420-ac

TIRMsFVkGWGAEYR

SMAD2 (human)	TIRMsFVkGWGAEYR	K420-ac
SMAD2 (mouse)	TIRMSFVKGWGAEYR	K420
SMAD2 (rat)	TIRMSFVKGWGAEYR	K420
SMAD2 (cow)	TIRMSFVKGWGAEYR	K420

S433

YRRQTVTSTPCWIEL

SMAD2 (human)	YRRQTVTSTPCWIEL	S433
SMAD2 (mouse)	YRRQTVTsTPCWIEL	S433-p
SMAD2 (rat)	YRRQTVTSTPCWIEL	S433
SMAD2 (cow)	YRRQTVTSTPCWIEL	S433

S458-p

KVLTQMGsPsVRCss

Upstream

Treatment

nocodazole

SMAD2 (human)	KVLTQMGsPsVRCss	S458-p
SMAD2 (mouse)	KVLTQMGsPSVRCSs	S458-p
SMAD2 (rat)	KVLTQMGSPSVRCSs	S458
SMAD2 (cow)	KVLTQMGSPSVRCSS	S458

S460-p

LTQMGsPsVRCssMs

Upstream

Treatment

nocodazole

SMAD2 (human)	LTQMGsPsVRCssMs	S460-p
SMAD2 (mouse)	LTQMGsPSVRCSsMs	S460
SMAD2 (rat)	LTQMGSPSVRCSsMs	S460
SMAD2 (cow)	LTQMGSPSVRCSSMS	S460

S464-p

GsPsVRCssMs____

Upstream

Downstream

Effects on Modified Protein

activity, induced
intracellular localization

Effects on Biological Processes:

transcription, altered
transcription, induced

Kinase, in vitro:

PAK2 (human)
TGFBR1 (human)

Putative in vivo kinases:

BMPR1B (human)
TGFBR1 (human)

Treatment

TGF-beta

SMAD2 (human)	GsPsVRCssMs____	S464-p
SMAD2 (mouse)	GsPSVRCSsMs____	S464
SMAD2 (rat)	GSPSVRCSsMs____	S464
SMAD2 (cow)	GSPSVRCSSMS____	S464

S465-p

sPsVRCssMs_____

Upstream

Downstream

SMAD2 (human)
S465-p

SMAD2 (mouse)
S465-p

SMAD2 (rat)
S465-p

SMAD2 (cow)
S465-p

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation

Effects on Biological Processes:

transcription, altered
transcription, induced

Kinase, in vitro:

PAK2 (human)
TGFBR1 (human)

Putative in vivo kinases:

BMPR1B (human)
TGFBR1 (human)

Regulatory protein:

Akt1 (human)
GDF11 (human)
GRK2 (human)
Lmr2 (human)
MSTN (human)
MURF1 (human)
PTPRK (human)
RHEB (human)
SMAD4 (human)
SMAD7 (human)
TGFBR1 (human)
TSC1 (human)

Treatment

5(S)-HETE
activin
AG538
ALK5_Inhibitor_I
angiotensin_2
anti-CD44
anti-HER2
ascorbic_acid
caffeic_acid
CRM197
decorin
EGF
FGF2
gefitinib
heregulin
hyaluronan
IGF-1
IL-6
insulin
LY294002
masoprocol
Nodal
oncostatin_M
picropodophyllin
rapamycin
SB202190
SB203580
siRNA
starvation_medium
TGF-beta
U0126
UV
Y27632

SMAD2 (human)	sPsVRCssMs_____	S465-p
SMAD2 (mouse)	sPSVRCSsMs_____	S465-p
SMAD2 (rat)	SPSVRCSsMs_____	S465-p
SMAD2 (cow)	SPSVRCSSMS_____	S465

S467-p

sVRCssMs_______

Upstream

Downstream

SMAD2 (human)
S467-p

SMAD2 (mouse)
S467-p

SMAD2 (rat)
S467-p

SMAD2 (cow)
S467-p

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation

Effects on Biological Processes:

transcription, altered
transcription, induced

Kinase, in vitro:

PAK2 (human)
TGFBR1 (human)

Putative in vivo kinases:

BMPR1B (human)
TGFBR1 (human)

Regulatory protein:

Akt1 (human)
GDF11 (human)
GRK2 (human)
Lmr2 (human)
MSTN (human)
MURF1 (human)
PTPRK (human)
RHEB (human)
SMAD4 (human)
SMAD7 (human)
TGFBR1 (human)
TSC1 (human)

Treatment

5(S)-HETE
activin
AG538
ALK5_Inhibitor_I
angiotensin_2
anti-CD44
anti-HER2
ascorbic_acid
caffeic_acid
CRM197
decorin
EGF
FGF2
gefitinib
heregulin
hyaluronan
IGF-1
IL-6
insulin
LY294002
masoprocol
Nodal
oncostatin_M
picropodophyllin
rapamycin
SB202190
SB203580
siRNA
starvation_medium
TGF-beta
U0126
UV
Y27632

SMAD2 (human)	sVRCssMs_______	S467-p
SMAD2 (mouse)	SVRCSsMs_______	S467-p
SMAD2 (rat)	SVRCSsMs_______	S467-p
SMAD2 (cow)	SVRCSSMS_______	S467