eNOS (cow)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

S58-p

DHSPAPNsPTLTRPP

Upstream

Kinase, in vitro:

ERK2 (human)

eNOS (human)	gap	-
eNOS (mouse)	gap	-
eNOS (rat)	gap	-
eNOS (rabbit)	SPKPSSNSPPLTRPP	S62
eNOS (pig)	DHSPAPNSPPLTRPP	S58
eNOS (sheep)	DHSPAPSSPTLTRPP	S58
eNOS (cow)	DHSPAPNsPTLTRPP	S58-p

___MGNLKSVGQEPG

eNOS (human)	___MGNLkSVAQEPG	K5-m1
eNOS (mouse)	___MGNLKSVGQEPG	K5
eNOS (rat)	___MGNLKSVGQEPG	K5
eNOS (rabbit)	___MGNLKSVGQEPG	K5
eNOS (pig)	___MGNLKSVAQEPG	K5
eNOS (sheep)	___MGNLKSVGQEPG	K5
eNOS (cow)	___MGNLKSVGQEPG	K5

S33

CGKQGPASPAPEPSR

eNOS (human)	CGKQGPAtPAPEPSR	T33-p
eNOS (mouse)	CGKQGPASPAPEPSQ	S33
eNOS (rat)	CGKQGPASPAPEPSQ	S33
eNOS (rabbit)	CGKQGPASPAPEPSR	S33
eNOS (pig)	CGKQGPASPAPEASQ	S33
eNOS (sheep)	CGKQGPASPAPEPSW	S33
eNOS (cow)	CGKQGPASPAPEPSR	S33

gap

eNOS (human)	gap	-
eNOS (mouse)	SQAPAPPsPtRPAPD	S46-p
eNOS (rat)	SQAPVPPSPTRPAPD	S46
eNOS (rabbit)	SRAPASPPPTPPAPD	P46
eNOS (pig)	gap	-
eNOS (sheep)	gap	-
eNOS (cow)	gap	-

T46-p

SRAPAPAtPHAPDHS

Upstream

Kinase, in vitro:

ERK2 (human)

eNOS (human)	gap	-
eNOS (mouse)	APAPPsPtRPAPDHs	T48-p
eNOS (rat)	APVPPSPTRPAPDHS	T48
eNOS (rabbit)	APASPPPTPPAPDHS	T48
eNOS (pig)	gap	-
eNOS (sheep)	SWAPAPATPQAPDHS	T46
eNOS (cow)	SRAPAPAtPHAPDHS	T46-p

S53

tPHAPDHSPAPNsPT

eNOS (human)	LPPAPEHsPPSSPLT	S53-p
eNOS (mouse)	tRPAPDHsPPLtRPP	S55-p
eNOS (rat)	TRPAPDHSPPLTRPP	S55
eNOS (rabbit)	TPPAPDHSPKPSSNS	S55
eNOS (pig)	PAPAPDHSPAPNSPP	S53
eNOS (sheep)	TPQAPDHSPAPSSPT	S53
eNOS (cow)	tPHAPDHSPAPNsPT	S53

T62

APNsPTLTRPPEGPK

eNOS (human)	sPPSSPLTQPPEGPK	T60
eNOS (mouse)	PDHsPPLtRPPDGPR	T59-p
eNOS (rat)	PDHSPPLTRPPDGPK	T59
eNOS (rabbit)	SSNSPPLTRPPEGPK	T66
eNOS (pig)	APNSPPLTRPPEGPK	T62
eNOS (sheep)	APSSPTLTRPPEGPK	T62
eNOS (cow)	APNsPTLTRPPEGPK	T62

Y83-p

WELGSITyDTLCAQS

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Kinase, in vitro:

Src (human)

Putative in vivo kinases:

Src (human)

Regulatory protein:

PTEN (human)

Treatment

angiopoietin-1
ATP
bradykinin
CAIP
CIAP
ciclosporin
estradiol
PP2
S-1-P
scrambled inhibitory peptide
thapsigargin
vanadate
VEGF

eNOS (human)	WEVGSITyDTLSAQA	Y81-p
eNOS (mouse)	WEVGSITyDTLSAQA	Y80-p
eNOS (rat)	WEVGSITyDTLSAQA	Y80-p
eNOS (rabbit)	WEVGSITYDTLSAQA	Y87
eNOS (pig)	WEVGSITYDTLCAQS	Y83
eNOS (sheep)	WELGSITYDTLCAQS	Y83
eNOS (cow)	WELGSITyDTLCAQS	Y83-p

S104

TPRCCLGSLVLPRKL

eNOS (human)	TPRRCLGsLVFPRKL	S102-p
eNOS (mouse)	TSRRCLGSLVFPRKL	S101
eNOS (rat)	TPRRCLGSLVFPRKL	S101
eNOS (rabbit)	TQRRCLGSLVFPRKL	S108
eNOS (pig)	TPRRCLGSLVFPRKL	S104
eNOS (sheep)	TPRRCLGSLVLPRKL	S104
eNOS (cow)	TPRCCLGSLVLPRKL	S104

S116-p

RKLQTRPsPGPPPAE

Upstream

Downstream

eNOS (human)
S114-p

9575 : Phospho-eNOS (Ser113) Antibody

eNOS (cow)
S116-p

9575 : Phospho-eNOS (Ser113) Antibody

Effects on Modified Protein

enzymatic activity, inhibited
molecular association, regulation
phosphorylation

Kinase, in vitro:

ERK2 (human)

Phosphatases, in vitro:

PPP2CA (human)

Regulatory protein:

DHFRL1 (human)
eNOS (cow)
GCH1 (human)
PPP2R5D (rat)
PTEN (human)
RAC1 (human)

Treatment

7,8-dihydrobiopterin
angiopoietin-1
aphidicolin
apoA-I
atenolol
ATP
bradykinin
butoxamine
calphostin_C
calyculin_A
cAMP_analog
ciclosporin
FK506
fluid_shear_stress
HDL
high_cell_density
LDL
okadaic_acid
PP2
quercetin
racepinefrine
sapropterin
SR59230A
thapsigargin
troglitazone
U0126
vanadate
VEGF
wortmannin

eNOS (human)	RKLQGRPsPGPPAPE	S114-p
eNOS (mouse)	RKLQSRPTQGPSPTE	T113
eNOS (rat)	RKLQSRPTQGPsPTE	T113
eNOS (rabbit)	RKLQSRPTQGAPPPE	T120
eNOS (pig)	RKLQSRPSQGPPPTE	S116
eNOS (sheep)	RKLQTRPSQGPPPAE	S116
eNOS (cow)	RKLQTRPsPGPPPAE	S116-p

P120

TRPsPGPPPAEQLLS

eNOS (human)	GRPsPGPPAPEQLLS	P118
eNOS (mouse)	SRPTQGPSPTEQLLG	S117
eNOS (rat)	SRPTQGPsPTEQLLG	S117-p
eNOS (rabbit)	SRPTQGAPPPEQLLV	P124
eNOS (pig)	SRPSQGPPPTEQLLS	P120
eNOS (sheep)	TRPSQGPPPAEQLLS	P120
eNOS (cow)	TRPsPGPPPAEQLLS	P120

Y212

SAQEMFTYICNHIKY

eNOS (human)	SAQEMFTyICNHIKY	Y210-p
eNOS (mouse)	TAQEMFTYICNHIKY	Y209
eNOS (rat)	TAQEMFTYICNHIKY	Y209
eNOS (rabbit)	SAQEMFTYICNHIKY	Y216
eNOS (pig)	SAQEMFTYICNHIKY	Y212
eNOS (sheep)	SAQEMFTYICNHIKY	Y212
eNOS (cow)	SAQEMFTYICNHIKY	Y212

S262

GYRQQDGSVRGDPAN

eNOS (human)	GYRQQDGsVRGDPAN	S260-p
eNOS (mouse)	GYRQQDGSVRGDPAN	S259
eNOS (rat)	GYRQQDGSVRGDPAN	S259
eNOS (rabbit)	GYRQQDGSVRGDPAN	S266
eNOS (pig)	GYRQQDGSVRGDPAN	S262
eNOS (sheep)	GYRQQDGSVRGDPAN	S263
eNOS (cow)	GYRQQDGSVRGDPAN	S262

S485

QPDPWKGSATKGAGI

eNOS (human)	QPDPWKGSAAKGTGI	S483
eNOS (mouse)	QPDPWKGsAAKGAGI	S482-p
eNOS (rat)	QPDPWKGSAAKGTGI	S482
eNOS (rabbit)	QPDPWKGSGAKGTGI	S489
eNOS (pig)	QPDPWKGSAAKGTGI	S485
eNOS (sheep)	QPDPWKGSAAKGAGI	S486
eNOS (cow)	QPDPWKGSATKGAGI	S485

K496-ac

GAGITRKktFKEVAN

Upstream

Treatment

decosahexaenoic acid
nicotinamide
siRNA

eNOS (human)	GTGITRKKtFKEVAN	K494
eNOS (mouse)	GAGITRKKtFKEVAN	K493
eNOS (rat)	GTGITRKKtFKEVAN	K493
eNOS (rabbit)	GTGITRKKTFKEVAN	K500
eNOS (pig)	GTGIARKKtFKEVAN	K496
eNOS (sheep)	GAGITRKKtFKEVAN	K497
eNOS (cow)	GAGITRKktFKEVAN	K496-ac

T497-p

AGITRKktFKEVANA

Upstream

Downstream

eNOS (human)
T495-p

9574 : Phospho-eNOS (Thr495) Antibody

eNOS (mouse)
T494-p

9574 : Phospho-eNOS (Thr495) Antibody

Effects on Modified Protein

enzymatic activity, inhibited
molecular association, regulation

Kinase, in vitro:

PKCA (human)

Phosphatases, in vitro:

PPP1CA (human)
PPP2CA (human)

Putative in vivo kinases:

PKCA (cow)
PKCZ (human)

Regulatory protein:

eNOS (cow)
PKCZ (human)
PTEN (human)
RAC1 (human)
TIE2 (human)

Treatment

17-beta-estradiol
A23187
angiopoietin-1
angiotensin_2
aphidicolin
aPL
apoA-I
atenolol
ATP
BAPTA-AM
bisindolylmaleimide
bradykinin
butoxamine
calyculin_A
cAMP_analog
ciclosporin
colforsin
fluid_shear_stress
geldanamycin
Go_6976
H2O2
HDL
IBMX
IgG
insulin
ionomycin
lactacystin
LDL
LY333531
MG132
nebivolol
okadaic_acid
phorbol_ester
PKCnPS
PP2
pulsatile shear stress
quercetin
racepinefrine
radicicol
rottlerin
SKI-1
SR59230A
staurosporine
thapsigargin
troglitazone
U0126
vanadate
VEGF
wortmannin

eNOS (human)	TGITRKKtFKEVANA	T495-p
eNOS (mouse)	AGITRKKtFKEVANA	T494-p
eNOS (rat)	TGITRKKtFKEVANA	T494-p
eNOS (rabbit)	TGITRKKTFKEVANA	T501
eNOS (pig)	TGIARKKtFKEVANA	T497-p
eNOS (sheep)	AGITRKKtFKEVANA	T498-p
eNOS (cow)	AGITRKktFKEVANA	T497-p

K506-ac

KEVANAVkISASLMG

Upstream

Treatment

decosahexaenoic acid
nicotinamide
siRNA

eNOS (human)	KEVANAVKISAsLMG	K504
eNOS (mouse)	KEVANAVKISASLMG	K503
eNOS (rat)	KEVANAVKISASLMG	K503
eNOS (rabbit)	KEVANAVKISASLMG	K510
eNOS (pig)	KEVANAVKISASLMG	K506
eNOS (sheep)	KEVANAVKISASLMG	K507
eNOS (cow)	KEVANAVkISASLMG	K506-ac

S510

NAVkISASLMGTLMA

eNOS (human)	NAVKISAsLMGtVMA	S508-p
eNOS (mouse)	NAVKISASLMGTVMA	S507
eNOS (rat)	NAVKISASLMGTVMA	S507
eNOS (rabbit)	NAVKISASLMGTVMA	S514
eNOS (pig)	NAVKISASLMGTVMA	S510
eNOS (sheep)	NAVKISASLMGTLMA	S511
eNOS (cow)	NAVkISASLMGTLMA	S510

T514

ISASLMGTLMAKRVK

eNOS (human)	ISAsLMGtVMAKRVK	T512-p
eNOS (mouse)	ISASLMGTVMAKRVK	T511
eNOS (rat)	ISASLMGTVMAKRVK	T511
eNOS (rabbit)	ISASLMGTVMAKRVK	T518
eNOS (pig)	ISASLMGTVMAKRVK	T514
eNOS (sheep)	ISASLMGTLMAKRVK	T515
eNOS (cow)	ISASLMGTLMAKRVK	T514

S602-p

MSGPYNSsPRPEQHK

Upstream

Kinase, in vitro:

ERK2 (human)

eNOS (human)	MSGPYNSsPRPEQHK	S600-p
eNOS (mouse)	MSGPYNSSPRPEQHK	S599
eNOS (rat)	MSGPYNSSPRPEQHK	S599
eNOS (rabbit)	MSGPYNSSPRPEQHK	S606
eNOS (pig)	MSGPYNGSPRPEQHR	S602
eNOS (sheep)	MSGPYNSSPRPEQHR	S603
eNOS (cow)	MSGPYNSsPRPEQHK	S602-p

S617-p

SYKIRFNsVSCSDPL

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
molecular association, regulation
phosphorylation

Kinase, in vitro:

Akt1 (human)

Regulatory protein:

eNOS (cow)
PTEN (human)

Treatment

17-beta-estradiol
apoA-I
ATP
BAPTA-AM
bradykinin
calyculin_A
HDL
IBMX
ionomycin
KT5720
LDL
lovastatin
LY294002
pravastatin
quercetin
VEGF
wortmannin

eNOS (human)	SYKIRFNsISCSDPL	S615-p
eNOS (mouse)	SYKIRFNsVSCSDPL	S614-p
eNOS (rat)	SYKIRFNsVSCSDPL	S614-p
eNOS (rabbit)	SYKIRFNSVSCSDPL	S621
eNOS (pig)	SYKIRFNSVSCSDPL	S617
eNOS (sheep)	SYKIRFNSVSCSDPL	S618
eNOS (cow)	SYKIRFNsVSCSDPL	S617-p

S617

SYKIRFNSVSCSDPL

eNOS (human)	SYKIRFNsISCSDPL	S615-gl
eNOS (mouse)	SYKIRFNSVSCSDPL	S614
eNOS (rat)	SYKIRFNSVSCSDPL	S614
eNOS (rabbit)	SYKIRFNSVSCSDPL	S621
eNOS (pig)	SYKIRFNSVSCSDPL	S617
eNOS (sheep)	SYKIRFNSVSCSDPL	S618
eNOS (cow)	SYKIRFNSVSCSDPL	S617

S635-p

WRRKRKEsSNTDSAG

Upstream

Downstream

Effects on Modified Protein

acetylation
enzymatic activity, induced
phosphorylation

Regulatory protein:

eNOS (cow)
PTEN (human)

Treatment

angiopoietin-1
apoA-I
ATP
BAPTA-AM
bradykinin
calyculin_A
cAMP_analog
fluid_shear_stress
H-89
HDL
IBMX
ionomycin
KT5720
lactacystin
LDL
lovastatin
LY294002
MG132
mPKI
pravastatin
quercetin
vanadate
VEGF
wortmannin

eNOS (human)	WRRKRKEssNtDsAG	S633-p
eNOS (mouse)	WRRKRKEsSNtDSAG	S632-p
eNOS (rat)	WRRKRKEsSNTDSAG	S632-p
eNOS (rabbit)	WRRKRKESSNTDSAG	S639
eNOS (pig)	WRRKRKESSNTDSAG	S635
eNOS (sheep)	WRRKRKESSNTDSAG	S636
eNOS (cow)	WRRKRKEsSNTDSAG	S635-p

S636

RRKRKEsSNTDSAGA

eNOS (human)	RRKRKEssNtDsAGA	S634-p
eNOS (mouse)	RRKRKEsSNtDSAGA	S633
eNOS (rat)	RRKRKEsSNTDSAGA	S633
eNOS (rabbit)	RRKRKESSNTDSAGA	S640
eNOS (pig)	RRKRKESSNTDSAGA	S636
eNOS (sheep)	RRKRKESSNTDSAGA	S637
eNOS (cow)	RRKRKEsSNTDSAGA	S636

T638

KRKEsSNTDSAGALG

eNOS (human)	KRKEssNtDsAGALG	T636-p
eNOS (mouse)	KRKEsSNtDSAGALG	T635-p
eNOS (rat)	KRKEsSNTDSAGALG	T635
eNOS (rabbit)	KRKESSNTDSAGALG	T642
eNOS (pig)	KRKESSNTDSAGALG	T638
eNOS (sheep)	KRKESSNTDSAGALG	T639
eNOS (cow)	KRKEsSNTDSAGALG	T638

S640

KEsSNTDSAGALGTL

eNOS (human)	KEssNtDsAGALGTL	S638-p
eNOS (mouse)	KEsSNtDSAGALGTL	S637
eNOS (rat)	KEsSNTDSAGALGTL	S637
eNOS (rabbit)	KESSNTDSAGALGTL	S644
eNOS (pig)	KESSNTDSAGALGTL	S640
eNOS (sheep)	KESSNTDSAGALGTL	S641
eNOS (cow)	KEsSNTDSAGALGTL	S640

Y659

FGLGSRAYPHFCAFA

eNOS (human)	FGLGSRAyPHFCAFA	Y657-p
eNOS (mouse)	FGLGSRAyPHFCAFA	Y656-p
eNOS (rat)	FGLGSRAyPHFCAFA	Y656-p
eNOS (rabbit)	FGLGSRAYPHFCAFA	Y663
eNOS (pig)	FGLGSRAyPHFCAFA	Y659-p
eNOS (sheep)	FGLGSRAYPHFCAFA	Y660
eNOS (cow)	FGLGSRAYPHFCAFA	Y659

S740

KRQRYRLSTQAEGLQ

eNOS (human)	KRQRYRLsAQAEGLQ	S738-p
eNOS (mouse)	KRQRYRLSTQAESLQ	S737
eNOS (rat)	KRQRYRLSTQAESLQ	S737
eNOS (rabbit)	KRQRYRLSTQTEGLQ	S744
eNOS (pig)	KRQRYRLSAQAEGLQ	S740
eNOS (sheep)	KRQRYRLSTQAEGLQ	S743
eNOS (cow)	KRQRYRLSTQAEGLQ	S740

S740

KRQRYRLSTQAEGLQ

eNOS (human)	KRQRYRLsAQAEGLQ	S738-gl
eNOS (mouse)	KRQRYRLSTQAESLQ	S737
eNOS (rat)	KRQRYRLSTQAESLQ	S737
eNOS (rabbit)	KRQRYRLSTQTEGLQ	S744
eNOS (pig)	KRQRYRLSAQAEGLQ	S740
eNOS (sheep)	KRQRYRLSTQAEGLQ	S743
eNOS (cow)	KRQRYRLSTQAEGLQ	S740

K775

VENLQSSKSTRATIL

eNOS (human)	VENLQSSkSTRATIL	K773-ub
eNOS (mouse)	VENLQSSKSTRATIL	K772
eNOS (rat)	VENLQSSKSTRATIL	K772
eNOS (rabbit)	VENLQSSKSTRATIL	K779
eNOS (pig)	VENLQSSKSTRATIL	K775
eNOS (sheep)	VENLQSSKSTRATIL	K778
eNOS (cow)	VENLQSSKSTRATIL	K775

K836

VAVEQLEKGSPGGPP

eNOS (human)	VAVEQLEkGsPGGPP	K834-ub
eNOS (mouse)	VAVEQLEKGSPGGPP	K833
eNOS (rat)	VAVEQLEKGSPGGPP	K833
eNOS (rabbit)	VAVEQLEKGSPGGPP	K840
eNOS (pig)	VAVEQLEKGSPGGPP	K836
eNOS (sheep)	gap	-
eNOS (cow)	VAVEQLEKGSPGGPP	K836

S838

VEQLEKGSPGGPPPS

eNOS (human)	VEQLEkGsPGGPPPG	S836-p
eNOS (mouse)	VEQLEKGSPGGPPPG	S835
eNOS (rat)	VEQLEKGSPGGPPPG	S835
eNOS (rabbit)	VEQLEKGSPGGPPPS	S842
eNOS (pig)	VEQLEKGSPGGPPPS	S838
eNOS (sheep)	gap	-
eNOS (cow)	VEQLEKGSPGGPPPS	S838

T856

DPRLPPCTLRQALTF

eNOS (human)	DPRLPPCtLRQALTF	T854-p
eNOS (mouse)	DPRLPPCTLRQALTY	T853
eNOS (rat)	DPRLPPCTLRQALTY	T853
eNOS (rabbit)	DPRLPPCSLRQALTF	S860
eNOS (pig)	DPRLPPCTLRQALTF	T856
eNOS (sheep)	DPRLPPCTLRQALTF	T855
eNOS (cow)	DPRLPPCTLRQALTF	T856

T868-gl

LTFFLDItSPPSPRL

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced

Regulatory protein:

AMPKA1 (human)

Treatment

acadesine
low_glucose

eNOS (human)	LTFFLDItsPPSPQL	T866-gl
eNOS (mouse)	LTYFLDITSPPSPRL	T865
eNOS (rat)	LTYFLDITSPPSPRL	T865
eNOS (rabbit)	LTFFLDITSPSSPRL	T872
eNOS (pig)	LTFFLDITSPPSPRL	T868
eNOS (sheep)	LTFFLDITSPPSPRL	T867
eNOS (cow)	LTFFLDItSPPSPRL	T868-gl

S869

TFFLDItSPPSPRLL

eNOS (human)	TFFLDItsPPSPQLL	S867-gl
eNOS (mouse)	TYFLDITSPPSPRLL	S866
eNOS (rat)	TYFLDITSPPSPRLL	S866
eNOS (rabbit)	TFFLDITSPSSPRLL	S873
eNOS (pig)	TFFLDITSPPSPRLL	S869
eNOS (sheep)	TFFLDITSPPSPRLL	S868
eNOS (cow)	TFFLDItSPPSPRLL	S869

K906

PRRYEEWKWFRCPTL

eNOS (human)	PRRYEEWkWFRCPTL	K904-ub
eNOS (mouse)	PRRYEEWKWFSCPTL	K903
eNOS (rat)	PRRYEEWKWFRCPTL	K903
eNOS (rabbit)	PRRYEEWKWFRCPTL	K910
eNOS (pig)	PRRYEEWKWFRCPTL	K906
eNOS (sheep)	PRRYEEWKWFRCPTL	K905
eNOS (cow)	PRRYEEWKWFRCPTL	K906

K1037

RLHDIESKGLQPAPM

eNOS (human)	RLHDIESkGLQPTPM	K1035-ub
eNOS (mouse)	RLHDIEIKGLQPAPM	K1034
eNOS (rat)	RLHDIEIKGLQPAPM	K1034
eNOS (rabbit)	RLHDIESKGLQPAPL	K1041
eNOS (pig)	RLHDIESKGLQPAPM	K1037
eNOS (sheep)	RLHDIESKGLQRAAP	K1037
eNOS (cow)	RLHDIESKGLQPAPM	K1037

K1087

SREPDSPKTYVQDIL

eNOS (human)	SREPDNPKTYVQDIL	K1085
eNOS (mouse)	SRDPGSPKTYVQDLL	K1084
eNOS (rat)	SRDPGSPkTYVQDLL	K1084-ac
eNOS (rabbit)	SREPDSPKTYVQDIL	K1091
eNOS (pig)	SREPDSPKTYVQDIL	K1087
eNOS (sheep)	SREPDSPKTYVQDIL	K1087
eNOS (cow)	SREPDSPKTYVQDIL	K1087

K1087

SREPDSPKTYVQDIL

eNOS (human)	SREPDNPkTYVQDIL	K1085-ub
eNOS (mouse)	SRDPGSPKTYVQDLL	K1084
eNOS (rat)	SRDPGSPKTYVQDLL	K1084
eNOS (rabbit)	SREPDSPKTYVQDIL	K1091
eNOS (pig)	SREPDSPKTYVQDIL	K1087
eNOS (sheep)	SREPDSPKTYVQDIL	K1087
eNOS (cow)	SREPDSPKTYVQDIL	K1087

T1096

YVQDILRTELAAEVH

eNOS (human)	YVQDILRtELAAEVH	T1094-p
eNOS (mouse)	YVQDLLRTELAAEVH	T1093
eNOS (rat)	YVQDLLRTELAAEVH	T1093
eNOS (rabbit)	YVQDILRTELASEVH	T1100
eNOS (pig)	YVQDILRTELAAEVH	T1096
eNOS (sheep)	YVQDILRTELAAEVH	T1096
eNOS (cow)	YVQDILRTELAAEVH	T1096

T1177-p

EVTSRIRtQsFsLQE

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
phosphorylation

Treatment

angiotensin_2
L-NAME
RNH6270
YM-254890

eNOS (human)	EVTSRIRtQsFsLQE	T1175-p
eNOS (mouse)	EVTSRIRtQsFsLQE	T1174-p
eNOS (rat)	EVTSRIRtQsFsLQE	T1174-p
eNOS (rabbit)	EVTSRIRTQsFSLQE	T1181
eNOS (pig)	EVTSRIRtQsFSLQE	T1177-p
eNOS (sheep)	EVTSRIRTQSFSLQE	T1177
eNOS (cow)	EVTSRIRtQsFsLQE	T1177-p

S1179-p

TSRIRtQsFsLQERH

Upstream

Downstream

249

eNOS (human)
S1177-p

eNOS (mouse)
S1176-p

eNOS (rat)
S1176-p

eNOS (cow)
S1179-p

Effects on Modified Protein

acetylation
activity, induced
enzymatic activity, induced
molecular association, regulation
phosphorylation
protein conformation

Effects on Biological Processes:

apoptosis, inhibited
cell motility, altered

Kinase, in vitro:

Akt1 (human)

Phosphatases, in vitro:

PPP2CA (human)

Putative in vivo kinases:

Akt1 (human)
AMPKA1 (human)
PKACA (cow)
PKCD (human)

Regulatory protein:

Akt1 (human)
AMPKA1 (human)
DHFRL1 (human)
eNOS (cow)
GCH1 (human)
MKK6 (human)
P38A (human)
PDK1 (human)
PKCZ (human)
PPP2CA (human)
PPP2R5D (human)
PTEN (human)
RAC1 (human)

Treatment

17-beta-estradiol
20-HETE
5-HT
A23187
angiopoietin-1
angiotensin
antibody
aphidicolin
aPL
apoA-I
arcyriarubin_A
atenolol
ATP
BADGE
BAPTA-AM
bisindolylmaleimide
black_tea_polyphenols
BN52021
bradykinin
butoxamine
Ca(2+)
calphostin_C
calyculin_A
cAMP_analog
cholest-4-en-3-one
ciclosporin
colforsin
compound_C
CV3988
DDMS
dexamethasone
DMSO
EGTA
faslodex
FCCP
fluid_shear_stress
geldanamycin
genistein
GF109203X
ghrelin
glucose_starvation
Go_6983
GW_9662
H-89
H2O2
HDL
hyperglycemia
IBMX
IgG
insulin
ionomycin
KT5823
L-NAME
lactacystin
LAU8080
lovastatin
low_Ca(2+)
LY294002
LY333531
metformin
MG132
mifepristone
mPKI
nebivolol
okadaic_acid
ONOO(-)
PAF
PD98059
phorbol_ester
PKC-zeta_inhibitor
PKCnPS
PP1
PP2
prasterone
pravastatin
PTX
pulsatile shear stress
quercetin
racepinefrine
radicicol
Ro31-8220
rottlerin
SB203347
SB203580
siRNA
SKI-1
sphingosine_1-phosphate
SR59230A
thapsigargin
thrombin
TNF
troglitazone
U0126
U73122
U73343
vanadate
VEGF
W-7
wortmannin
YM-254890

eNOS (human)	TSRIRtQsFsLQERQ	S1177-p
eNOS (mouse)	TSRIRtQsFsLQERQ	S1176-p
eNOS (rat)	TSRIRtQsFsLQERQ	S1176-p
eNOS (rabbit)	TSRIRTQsFSLQERQ	S1183-p
eNOS (pig)	TSRIRtQsFSLQERH	S1179-p
eNOS (sheep)	TSRIRTQSFSLQERH	S1179
eNOS (cow)	TSRIRtQsFsLQERH	S1179-p

S1179

TSRIRtQSFsLQERH

eNOS (human)	TSRIRtQsFsLQERQ	S1177-gl
eNOS (mouse)	TSRIRtQSFsLQERQ	S1176
eNOS (rat)	TSRIRtQsFsLQERQ	S1176-gl
eNOS (rabbit)	TSRIRTQSFSLQERQ	S1183
eNOS (pig)	TSRIRtQSFSLQERH	S1179
eNOS (sheep)	TSRIRTQSFSLQERH	S1179
eNOS (cow)	TSRIRtQSFsLQERH	S1179

S1181-p

RIRtQsFsLQERHLR

Upstream

Downstream

Effects on Modified Protein

enzymatic activity, induced
molecular association, regulation

Regulatory protein:

IRS1 (rat)

Treatment

insulin
phorbol_ester
VEGF

eNOS (human)	RIRtQsFsLQERQLR	S1179-p
eNOS (mouse)	RIRtQsFsLQERQLR	S1178-p
eNOS (rat)	RIRtQsFsLQERQLR	S1178-p
eNOS (rabbit)	RIRTQsFSLQERQLR	S1185
eNOS (pig)	RIRtQsFSLQERHLR	S1181
eNOS (sheep)	RIRTQSFSLQERHLR	S1181
eNOS (cow)	RIRtQsFsLQERHLR	S1181-p