MLKL (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

S52

LQDQGKRsVPSEKLt

MLKL (human)	LQDQGKRsVPSEKLt	S52
MLKL (mouse)	LQAQGKKNLPDDITA	N52
MLKL iso2 (mouse)	LQAQGKKNLPDDITA	N52
MLKL (rat)	LQAQEKKSLPSDITA	S52

T59

sVPSEKLtTAMNRFK

MLKL (human)	sVPSEKLtTAMNRFK	T59
MLKL (mouse)	KNLPDDITAALGRFD	T58
MLKL iso2 (mouse)	KNLPDDITAALGRFD	T58
MLKL (rat)	KSLPSDITAALGRFE	T58

S80

NGEIEKFSNRSNICR

MLKL (human)	NGEIEKFSNRSNICR	S80
MLKL (mouse)	NQQIEKFsKKSHIWK	S79
MLKL iso2 (mouse)	NQQIEKFSKKSHIWK	S79
MLKL (rat)	RQQIEKFSQKSNIWK	S79

S92

ICRFLTAsQDKILFK

MLKL (human)	ICRFLTAsQDKILFK	S92
MLKL (mouse)	IWKFVSVGNDKILFH	G91
MLKL iso2 (mouse)	IWKFVSVGNDKILFH	G91
MLKL (rat)	IWKFATVGSDKILFH	G91

S106

KDVNRKLsDVWKELS

MLKL (human)	KDVNRKLsDVWKELS	S106
MLKL (mouse)	HEVNEKLRDVWEELL	R105
MLKL iso2 (mouse)	HEVNEKLRDVWEELL	R105
MLKL (rat)	HEVNEKLRDIWEELL	R105

S125

VEQRMPVsPIsQGAS

Upstream

Treatment

nocodazole

MLKL (human)	VEQRMPVsPIsQGAS	S125
MLKL (mouse)	VYHWNTVsDVSQPAS	S124
MLKL iso2 (mouse)	VYHWNTVSDVSQPAS	S124
MLKL (rat)	VDQWKSDSEVSQPAS	S124

S128

RMPVsPIsQGASWAQ

MLKL (human)	RMPVsPIsQGASWAQ	S128
MLKL (mouse)	WNTVsDVSQPASWQQ	S127
MLKL iso2 (mouse)	WNTVSDVSQPASWQQ	S127
MLKL (rat)	WKSDSEVSQPASWQQ	S127

S161

DNEKIEAsLRRLEIN

MLKL (human)	DNEKIEAsLRRLEIN	S161
MLKL (mouse)	GNENMKVILMQLQIs	I151
MLKL iso2 (mouse)	GNENMKVILMQLQIS	I151
MLKL (rat)	GNENMKVILVKLQIS	I151

N168

sLRRLEINMKEIKET

MLKL (human)	sLRRLEINMKEIKET	N168
MLKL (mouse)	ILMQLQIsVEEINKT	S158
MLKL iso2 (mouse)	ILMQLQISVEEINKT	S158
MLKL (rat)	ILVKLQISMEEIKEA	S158

S239

FKKLQAGSIAIVRQt

MLKL (human)	FKKLQAGSIAIVRQt	S239
MLKL (mouse)	FNNPQAEsVGIVRFT	S228
MLKL iso2 (mouse)	FNNPQAESVGIVRFT	S228
MLKL (rat)	FNNPKAESVGIVRLT	S228

T246

SIAIVRQtFNKEIKT

MLKL (human)	SIAIVRQtFNKEIKT	T246
MLKL (mouse)	sVGIVRFTFNDEIKT	T235
MLKL iso2 (mouse)	SVGIVRFTFNDEIKT	T235
MLKL (rat)	SVGIVRLTFNEEIKT	T235

S259

KTMKKFESPNILRIF

MLKL (human)	KTMKKFESPNILRIF	S259
MLKL (mouse)	KTMKKFDsPNILRIF	S248
MLKL iso2 (mouse)	KTMKKFDSPNILRIF	S248
MLKL (rat)	KTMKKFDSPNILRIF	S248

T302

LDREKDLtLGKRMVL

MLKL (human)	LDREKDLtLGKRMVL	T302
MLKL (mouse)	LDREKDLTMSVRSLL	T291
MLKL iso2 (mouse)	LDREKDLTMSVRSLL	T291
MLKL (rat)	LDRDKDLTMSVRSLL	T291

S334

ELHGKIRsSNFLVTQ

MLKL (human)	ELHGKIRsSNFLVTQ	S334
MLKL (mouse)	TLHRNISSSSFLVAG	S321
MLKL iso2 (mouse)	TLHRNISSSSFLVAG	S321
MLKL (rat)	TLHGNISSSSFLVAG	S321

T357

FELRKTQtsMSLGTt

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation
protein conformation

Effects on Biological Processes:

apoptosis, induced
signaling pathway regulation

Kinase, in vitro:

RIPK3 (human)

Putative in vivo kinases:

RIPK3 (human)

Regulatory protein:

TIF1B (human)

Treatment

LPS
Necrostatin-1
TNF
Z-VAD-FMK

MLKL (human)	FELRKTQtsMSLGTt	T357
MLKL (mouse)	FELSKTQNsIsRtAK	N344
MLKL iso2 (mouse)	FELSKTQNSISRTAK	N344
MLKL (rat)	FELSKTQTsISRATK	T344

S358

ELRKTQtsMSLGTtR

Upstream

Downstream

MLKL (human)
S358

MLKL (mouse)
S345

Effects on Modified Protein

intracellular localization
molecular association, regulation
protein conformation

Effects on Biological Processes:

apoptosis, induced
carcinogenesis, inhibited
cell motility, inhibited
signaling pathway regulation
transcription, induced

Kinase, in vitro:

RIPK3 (human)

Putative in vivo kinases:

RIPK3 (human)

Regulatory protein:

RIPK3 (human)
TIF1B (human)

Treatment

adriamycin
Akt_inhibitor_VIII
BV6
GSK'843
GSK'872
LPS
LY294002
Necrostatin-1
phenhydan
primidone
resibufogenin
siRNA
SM-164
smac
thrombin
TNF
virus infection
wortmannin
Z-VAD-FMK

MLKL (human)	ELRKTQtsMSLGTtR	S358
MLKL (mouse)	ELSKTQNsIsRtAKS	S345
MLKL iso2 (mouse)	ELSKTQNSISRTAKS	S345
MLKL (rat)	ELSKTQTsISRATKS	S345

S360

RKTQtsMSLGTtREK

MLKL (human)	RKTQtsMSLGTtREK	S360
MLKL (mouse)	SKTQNsIsRtAKSTK	S347
MLKL iso2 (mouse)	SKTQNSISRTAKSTK	S347
MLKL (rat)	SKTQTsISRATKSTK	S347

G362

TQtsMSLGTtREKTD

MLKL (human)	TQtsMSLGTtREKTD	G362
MLKL (mouse)	TQNsIsRtAKSTKAE	T349
MLKL iso2 (mouse)	TQNSISRTAKSTKAE	T349
MLKL (rat)	TQTsISRATKSTKAE	A349

T364

tsMSLGTtREKTDRV

MLKL (human)	tsMSLGTtREKTDRV	T364
MLKL (mouse)	NsIsRtAKSTKAERS	K351
MLKL iso2 (mouse)	NSISRTAKSTKAERS	K351
MLKL (rat)	TsISRATKSTKAERF	K351

S373

EKTDRVKstAyLSPQ

MLKL (human)	EKTDRVKstAyLSPQ	S373
MLKL (mouse)	TKAERSSstIYVSPE	S360
MLKL iso2 (mouse)	TKAERSSSTIYVSPE	S360
MLKL (rat)	TKAERFSSTAYVSPE	S360

T374

KTDRVKstAyLSPQE

MLKL (human)	KTDRVKstAyLSPQE	T374
MLKL (mouse)	KAERSSstIYVSPER	T361
MLKL iso2 (mouse)	KAERSSSTIYVSPER	T361
MLKL (rat)	KAERFSSTAYVSPER	T361

Y376

DRVKstAyLSPQELE

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein stabilization

Kinase, in vitro:

Tyro3 (human)

Putative in vivo kinases:

AXL (human)
Mer (human)
Tyro3 (human)

Treatment

BMS-777607
LDC1267
NSA
SM-164
TNF
Z-VAD-FMK

MLKL (human)	DRVKstAyLSPQELE	Y376
MLKL (mouse)	ERSSstIYVSPERLK	Y363
MLKL iso2 (mouse)	ERSSSTIYVSPERLK	Y363
MLKL (rat)	ERFSSTAYVSPERLQ	Y363

S393

FYQYDVKsEIYSFGI

MLKL (human)	FYQYDVKsEIYSFGI	S393
MLKL (mouse)	FCLYDIKAEIYSFGI	A380
MLKL iso2 (mouse)	FCLYDIKAEIYSFGI	A380
MLKL (rat)	FCKYDTKAEIYSFGI	A380

S417

IPFQGCNsEKIRKLV

MLKL (human)	IPFQGCNsEKIRKLV	S417
MLKL (mouse)	IPFEGCDSKKIRELV	S404
MLKL iso2 (mouse)	IPFEGCDSKKIRELV	S404
MLKL (rat)	IPFEGCDSEKIYELV	S404

S454

ECRAHDPSVRPSVDE

MLKL (human)	ECRAHDPSVRPSVDE	S454
MLKL (mouse)	ECRAHEPsQRPSVDG	S441
MLKL iso2 (mouse)	ECRAHEPSQRPSVDG	S441
MLKL (rat)	ECRAHEPSNRPSADG	S441

S467

DEILKKLsTFSK___

MLKL (human)	DEILKKLsTFSK___	S467
MLKL (mouse)	ERILERLSAVEESTD	S462
MLKL iso2 (mouse)	DGILERLSAVEESTD	S454
MLKL (rat)	DGILERLPAIEDSMD	P454

S215

LLRENEVsTLYKGEY

MLKL (human)	LLRENEVsTLYKGEY	S215
MLKL (mouse)	KLKTSKMSTIYRGEY	S204
MLKL iso2 (mouse)	KLKTSKMSTIYRGEY	S204
MLKL (rat)	KLKTNKLSTIYRGEY	S204