MRLC2 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

______MSSKKAKTK

MRLC2 (human)	______MSSKKAKTK	S2
MRLC2 (mouse)	______MssKKAKTK	S2-p
MRLC2 (rat)	______MSSKKAKTK	S2
MRLC2 (cow)	______MSSKRAKTK	S2

_____MSSKKAKTKt

MRLC2 (human)	_____MSSKKAKTKt	S3
MRLC2 (mouse)	_____MssKKAKTKT	S3-p
MRLC2 (rat)	_____MSSKKAKTKT	S3
MRLC2 (cow)	_____MSSKRAKTKT	S3

T10-p

SKKAKTKttKKRPQR

MRLC2 (human)	SKKAKTKttKKRPQR	T10-p
MRLC2 (mouse)	sKKAKTKTTKKRPQR	T10
MRLC2 (rat)	SKKAKTKTTKKRPQR	T10
MRLC2 (cow)	SKRAKTKTTKKRPQR	T10

T11-p

KKAKTKttKKRPQRA

MRLC2 (human)	KKAKTKttKKRPQRA	T11-p
MRLC2 (mouse)	KKAKTKTTKKRPQRA	T11
MRLC2 (rat)	KKAKTKTTKKRPQRA	T11
MRLC2 (cow)	KRAKTKTTKKRPQRA	T11

T19-p

KKRPQRAtsNVFAMF

Upstream

Downstream

331

Effects on Biological Processes:

cell motility, altered
cytoskeletal reorganization

Kinase, in vitro:

DAPK3 (human)
DLK (human)
ILK (human)
ROCK1 (human)
smMLCK (human)

Putative in vivo kinases:

DAPK3 (human)
ROCK1 (human)

Regulatory protein:

ACTG1 (human)
AFAP1L1 (human)
ARHGAP22 (human)
Cas-L (human)
DOCK3 (human)
HRas (mouse)
ITGB3 (human)
PLK3 (human)
RAC1 (human)
ROCK1 (human)
Src (human)
WAVE2 (human)

Treatment

anti-EPCR
APC
BX795
calyculin_A
cell_detachment
EDTA
EGF
ephrin_A1
exoenzyme_C3
fibronectin
GF109203X
integrilin
metastatic potential
miRNA
nocodazole
okadaic_acid
phorbol_ester
PP2
PP3
serum
serum_starvation
siRNA
Su11274
thrombin
vanadate
Y27632

MRLC2 (human)	KKRPQRAtsNVFAMF	T19-p
MRLC2 (mouse)	KKRPQRAtsNVFAMF	T19-p
MRLC2 (rat)	KKRPQRAtsNVFAMF	T19-p
MRLC2 (cow)	KKRPQRAtsNVFAMF	T19-p

S20-p

KRPQRAtsNVFAMFD

Upstream

Downstream

357

Effects on Modified Protein

intracellular localization

Effects on Biological Processes:

cell motility, altered
cytoskeletal reorganization

Kinase, in vitro:

AurB (human)
DAPK1 (human)
DAPK1 (mouse)
DAPK3 (human)
DLK (human)
ILK (human)
ROCK1 (human)
smMLCK (human)

Putative in vivo kinases:

DAPK3 (human)
ILK (human)
ROCK1 (human)
smMLCK (human)

Regulatory protein:

ACTG1 (human)
AFAP1L1 (human)
ARHGAP22 (human)
Cas-L (human)
DLC1 (human)
DOCK3 (human)
FAM40B (human)
HRas (mouse)
ITGB3 (human)
MST3 (human)
MST4 (human)
PLK3 (human)
RAC1 (human)
RAF1 (human)
ROCK1 (human)
SG2NA (human)
Src (human)
STRIP1 (human)
WAVE2 (human)

Treatment

5-HT
ACh
angiotensin_2
anti-CD3
anti-EPCR
APC
arachidonic_acid
BX795
C3_toxin
calyculin_A
cell_detachment
Clostridium_toxin_B
compound_C
CPA
DPDPE
EDTA
EGF
ephrin_A1
exoenzyme_C3
fibronectin
GF109203X
HA-1077
integrilin
isoproterenol
LY294002
M-1
metastatic potential
miRNA
NaB
nocodazole
okadaic_acid
ONO-3708
PD184352
phorbol_ester
PIK
PP2
PP3
racepinefrine
SB203580
serum
serum_starvation
siRNA
somatostatin
Su11274
SU6656
thrombin
TxA2
vanadate
Y27632
yohimbine

MRLC2 (human)	KRPQRAtsNVFAMFD	S20-p
MRLC2 (mouse)	KRPQRAtsNVFAMFD	S20-p
MRLC2 (rat)	KRPQRAtsNVFAMFD	S20-p
MRLC2 (cow)	KRPQRAtsNVFAMFD	S20-p

S29-p

VFAMFDQsQIQEFKE

MRLC2 (human)	VFAMFDQsQIQEFKE	S29-p
MRLC2 (mouse)	VFAMFDQsQIQEFKE	S29-p
MRLC2 (rat)	VFAMFDQSQIQEFKE	S29
MRLC2 (cow)	VFAMFDQSQIQEFKE	S29

K51-ac

NRDGFIDkEDLHDML

MRLC2 (human)	NRDGFIDkEDLHDML	K51-ac
MRLC2 (mouse)	NRDGFIDKEDLHDML	K51
MRLC2 (rat)	NRDGFIDkEDLHDML	K51-ac
MRLC2 (cow)	NRDGFIDKEDLHDML	K51

K51-ub

NRDGFIDkEDLHDML

MRLC2 (human)	NRDGFIDkEDLHDML	K51-ub
MRLC2 (mouse)	NRDGFIDkEDLHDML	K51-ub
MRLC2 (rat)	NRDGFIDKEDLHDML	K51
MRLC2 (cow)	NRDGFIDKEDLHDML	K51

T97-p

FGEKLNGtDPEDVIR

MRLC2 (human)	FGEKLNGtDPEDVIR	T97-p
MRLC2 (mouse)	FGEKLNGTDPEDVIR	T97
MRLC2 (rat)	FGEKLNGTDPEDVIR	T97
MRLC2 (cow)	FGEKLNGTDPEDVIR	T97

T135-p

TTMGDRFtDEEVDEL

Upstream

Kinase, in vitro:

CK2A1 (human)

MRLC2 (human)	TTMGDRFtDEEVDEL	T135-p
MRLC2 (mouse)	TTMGDRFtDEEVDEL	T135-p
MRLC2 (rat)	TTMGDRFTDEEVDEL	T135
MRLC2 (cow)	TTMGDRFTDEEVDEL	T135

Y143-p

DEEVDELyREAPIDk

MRLC2 (human)	DEEVDELyREAPIDk	Y143-p
MRLC2 (mouse)	DEEVDELYREAPIDk	Y143
MRLC2 (rat)	DEEVDELYREAPIDK	Y143
MRLC2 (cow)	DEEVDELYREAPIDK	Y143

K150-ub

yREAPIDkkGNFNyI

Upstream

Treatment

MG132

MRLC2 (human)	yREAPIDkkGNFNyI	K150-ub
MRLC2 (mouse)	YREAPIDkkGNFNYI	K150-ub
MRLC2 (rat)	YREAPIDKKGNFNYI	K150
MRLC2 (cow)	YREAPIDKKGNFNYI	K150

K151-ub

REAPIDkkGNFNyIE

Upstream

Treatment

MG132

MRLC2 (human)	REAPIDkkGNFNyIE	K151-ub
MRLC2 (mouse)	REAPIDkkGNFNYIE	K151-ub
MRLC2 (rat)	REAPIDKKGNFNYIE	K151
MRLC2 (cow)	REAPIDKKGNFNYIE	K151

Y156-p

DkkGNFNyIEFTRIL

MRLC2 (human)	DkkGNFNyIEFTRIL	Y156-p
MRLC2 (mouse)	DkkGNFNYIEFTRIL	Y156
MRLC2 (rat)	DKKGNFNYIEFTRIL	Y156
MRLC2 (cow)	DKKGNFNYIEFTRIL	Y156

K164-ub

IEFTRILkHGAkDKD

MRLC2 (human)	IEFTRILkHGAkDKD	K164-ub
MRLC2 (mouse)	IEFTRILKHGAKDKD	K164
MRLC2 (rat)	IEFTRILKHGAKDKD	K164
MRLC2 (cow)	IEFTRILKHGAKDKD	K164

K168-ac

RILkHGAkDKDD___

MRLC2 (human)	RILkHGAkDKDD___	K168-ac
MRLC2 (mouse)	RILKHGAKDKDD___	K168
MRLC2 (rat)	RILKHGAKDKDD___	K168
MRLC2 (cow)	RILKHGAKDKDD___	K168