Other Species / Isoforms
  SMAD3 (human)      LTP 

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

 HTP 

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

 
T8-p
MSSILPFtPPIVkRL
Upstream
Downstream
5 13
Effects on Biological Processes:
  • cell cycle regulation
  • transcription, altered
  • transcription, inhibited
Putative in vivo kinases:
  • CDK2 (mouse)
  • CDK4 (human)
  • CDK4 (mouse)
Treatment
  • SB203580
  • TGF-beta
  • Y27632
SMAD3 (human) MSSILPFtPPIVkRL T8-p
SMAD3 (mouse) MSSILPFtPPIVKRL T8-p
SMAD3 (rat) MSSILPFtPPIVKRL T8-p
K13-ub
PFtPPIVkRLLGWkK
0 4
SMAD3 (human) PFtPPIVkRLLGWkK K13-ub
SMAD3 (mouse) PFtPPIVKRLLGWKK K13
SMAD3 (rat) PFtPPIVKRLLGWKK K13
K19-ac
VkRLLGWkKGEQNGQ
0 5
SMAD3 (human) VkRLLGWkKGEQNGQ K19-ac
SMAD3 (mouse) VKRLLGWKKGEQNGQ K19
SMAD3 (rat) VKRLLGWKKGEQNGQ K19
K29-ac
EQNGQEEkWCEKAVK
0 1
SMAD3 (human) EQNGQEEkWCEKAVK K29-ac
SMAD3 (mouse) EQNGQEEKWCEKAVK K29
SMAD3 (rat) EQNGQEEKWCEKAVK K29
K63-ub
TTQNVNTkCITIPRS
0 1
SMAD3 (human) TTQNVNTkCITIPRS K63-ub
SMAD3 (mouse) TTQNVNTKCITIPRS K63
SMAD3 (rat) TTQNVNTKCITIPRS K63
T132-p
YHYQRVEtPVLPPVL
0 8
SMAD3 (human) YHYQRVEtPVLPPVL T132-p
SMAD3 (mouse) YHYQRVEtPVLPPVL T132-p
SMAD3 (rat) YHYQRVETPVLPPVL T132
T179-p
PQSNIPEtPPPGYLS
Upstream
Downstream
15 0
Effects on Modified Protein
  • activity, inhibited
  • intracellular localization
  • molecular association, regulation
Effects on Biological Processes:
  • carcinogenesis, induced
  • cell cycle regulation
  • cell growth, altered
  • cell motility, induced
  • signaling pathway regulation
  • transcription, altered
  • transcription, induced
  • transcription, inhibited
Kinase, in vitro:
  • CDK8 (human)
  • CDK9 (human)
  • ERK2 (human)
  • OSR1 (human)
Putative in vivo kinases:
  • Akt1 (human)
  • CDK2 (mouse)
  • CDK4 (human)
  • CDK4 (mouse)
  • CDK8 (human)
  • CDK9 (human)
  • OSR1 (human)
Regulatory protein:
  • HRas (human)
  • MEK1 (human)
  • NOX4 (human)
  • SMAD4 (human)
  • TGFB1 (human)
Treatment
  • alvocidib
  • BMP2
  • DPI
  • EGF
  • H2O2
  • kaempferol
  • LTB4
  • PD98059
  • SB203580
  • SB431542
  • siRNA
  • TGF-beta
  • U0126
  • wortmannin
  • Y27632
SMAD3 (human) PQSNIPEtPPPGYLS T179-p
SMAD3 (mouse) PQSNIPETPPPGYLS T179
SMAD3 (rat) PQSNIPETPPPGYLS T179
S204-p
NHSMDAGsPNLsPNP
Upstream
Downstream
17 0
Effects on Modified Protein
  • activity, inhibited
  • intracellular localization
  • molecular association, regulation
Effects on Biological Processes:
  • cell growth, altered
  • signaling pathway regulation
  • transcription, altered
  • transcription, inhibited
Kinase, in vitro:
  • ERK2 (human)
  • MELK (mouse)
Putative in vivo kinases:
  • CDK4 (human)
  • MELK (mouse)
Regulatory protein:
  • HRas (human)
  • MEK1 (human)
Treatment
  • EGF
  • PD98059
  • SB203580
  • TGF-beta
  • U0126
  • wortmannin
  • Y27632
SMAD3 (human) NHSMDAGsPNLsPNP S204-p
SMAD3 (mouse) NHSMDAGsPNLSPNP S204-p
SMAD3 (rat) NHSMDAGSPNLSPNP S204
S208-p
DAGsPNLsPNPMsPA
Upstream
Downstream
17 1
Effects on Modified Protein
  • activity, inhibited
  • intracellular localization
  • phosphorylation
  • protein degradation
Effects on Biological Processes:
  • cell growth, altered
  • cell growth, induced
  • cell motility, inhibited
  • signaling pathway regulation
  • transcription, altered
  • transcription, inhibited
Kinase, in vitro:
  • CDK8 (human)
  • CDK9 (human)
  • ERK2 (human)
  • NLK (human)
Putative in vivo kinases:
  • CDK4 (human)
  • ERK1 (rat)
  • ERK2 (rat)
  • NLK (human)
Regulatory protein:
  • HRas (human)
  • MEK1 (human)
  • NOX4 (human)
Treatment
  • alvocidib
  • bacterial biofilm
  • DPI
  • EGF
  • FSL-1
  • gelatin
  • H2O2
  • HB-EGF
  • LTB4
  • PD184352
  • PD98059
  • SB203580
  • SB431542
  • siRNA
  • TGF-alpha
  • TGF-beta
  • U0126
  • wortmannin
  • Y27632
SMAD3 (human) DAGsPNLsPNPMsPA S208-p
SMAD3 (mouse) DAGsPNLSPNPMSPA S208
SMAD3 (rat) DAGSPNLSPNPMSPA S208
S213-p
NLsPNPMsPAHNNLD
Upstream
Downstream
11 0
Effects on Modified Protein
  • intracellular localization
Effects on Biological Processes:
  • cell cycle regulation
  • cell growth, altered
  • transcription, altered
  • transcription, inhibited
Kinase, in vitro:
  • CDK8 (human)
  • CDK9 (human)
  • ERK2 (human)
Putative in vivo kinases:
  • CDK2 (mouse)
  • CDK4 (human)
  • CDK4 (mouse)
Regulatory protein:
  • HRas (human)
  • MEK1 (human)
Treatment
  • EGF
  • gelatin
  • PD98059
  • SB203580
  • TGF-beta
  • wortmannin
  • Y27632
SMAD3 (human) NLsPNPMsPAHNNLD S213-p
SMAD3 (mouse) NLSPNPMSPAHNNLD S213
SMAD3 (rat) NLSPNPMSPAHNNLD S213
S264-p
VDGFTDPsNSERFCL
Downstream
1 0
Effects on Modified Protein
  • activity, inhibited
  • phosphorylation
  • protein stabilization
Effects on Biological Processes:
  • cell differentiation, inhibited
  • cell growth, induced
  • signaling pathway regulation
  • transcription, inhibited
SMAD3 (human) VDGFTDPsNSERFCL S264-p
SMAD3 (mouse) VDGFTDPSNSERFCL S264
SMAD3 (rat) VDGFTDPSNSERFCL S264
S309-p
EVFAECLsDSAIFVQ
2 1
SMAD3 (human) EVFAECLsDSAIFVQ S309-p
SMAD3 (mouse) EVFAECLsDSAIFVQ S309-p
SMAD3 (rat) EVFAECLSDSAIFVQ S309
K378-ac
TIRMSFVkGWGAEYR
Upstream
Downstream
1 4
Effects on Modified Protein
  • activity, induced
Effects on Biological Processes:
  • transcription, induced
Treatment
  • TGF-beta
SMAD3 (human) TIRMSFVkGWGAEYR K378-ac
SMAD3 (mouse) TIRMSFVKGWGAEYR K378
SMAD3 (rat) TIRMSFVKGWGAEYR K378
T388-p
GAEYRRQtVTSTPCW
Upstream
Downstream
3 1
Effects on Modified Protein
  • molecular association, regulation
Effects on Biological Processes:
  • transcription, induced
  • transcription, inhibited
Treatment
  • PD98059
  • TGF-beta
SMAD3 (human) GAEYRRQtVTSTPCW T388-p
SMAD3 (mouse) GAEYRRQtVTSTPCW T388-p
SMAD3 (rat) GAEYRRQTVTSTPCW T388
T412-p
QWLDKVLtQMGsPsI
0 1
SMAD3 (human) QWLDKVLtQMGsPsI T412-p
SMAD3 (mouse) QWLDKVLTQMGSPSI T412
SMAD3 (rat) QWLDKVLTQMGSPSI T412
S416-p
KVLtQMGsPsIRCss
Downstream
1 3
Effects on Modified Protein
  • intracellular localization
SMAD3 (human) KVLtQMGsPsIRCss S416-p
SMAD3 (mouse) KVLTQMGSPSIRCSs S416
SMAD3 (rat) KVLTQMGSPSIRCSs S416
S418-p
LtQMGsPsIRCssVs
Upstream
Downstream
2 1
Effects on Modified Protein
  • activity, inhibited
  • intracellular localization
  • protein degradation
  • ubiquitination
Effects on Biological Processes:
  • cell growth, inhibited
  • signaling pathway regulation
Kinase, in vitro:
  • CK1G2 (human)
Putative in vivo kinases:
  • CK1G2 (human)
Treatment
  • MG132
  • TGF-beta
SMAD3 (human) LtQMGsPsIRCssVs S418-p
SMAD3 (mouse) LTQMGSPSIRCSsVs S418
SMAD3 (rat) LTQMGSPSIRCSsVs S418
S422-p
GsPsIRCssVs____
Upstream
Downstream
2 0
Effects on Biological Processes:
  • transcription, altered
  • transcription, induced
Treatment
  • cAMP_analog
  • TGF-beta
SMAD3 (human) GsPsIRCssVs____ S422-p
SMAD3 (mouse) GSPSIRCSsVs____ S422
SMAD3 (rat) GSPSIRCSsVs____ S422
S423-p
sPsIRCssVs_____
Upstream
Downstream
30 5
SMAD3 (human)
S423-p
SMAD3 (mouse)
S423-p
SMAD3 (rat)
S423-p
Effects on Modified Protein
  • activity, induced
  • protein conformation
Effects on Biological Processes:
  • cell motility, altered
  • signaling pathway regulation
  • transcription, altered
Regulatory protein:
  • GDF11 (human)
  • HTRA1 (human)
  • MEK1 (human)
  • MSTN (human)
  • MURF1 (human)
  • RHEB (human)
  • SMAD3 (human)
  • SP1 (human)
  • TSC1 (human)
Treatment
  • activin
  • ALK5_Inhibitor_I
  • angiotensin_2
  • anti-CD44
  • CRM197
  • EGF
  • FGF2
  • gelatin
  • heregulin
  • hyaluronan
  • IGF-1
  • insulin
  • LY2109761
  • LY294002
  • Nodal
  • oncostatin_M
  • rapamycin
  • SB203580
  • SB431542
  • starvation_medium
  • TGF-beta
  • Y27632
SMAD3 (human) sPsIRCssVs_____ S423-p
SMAD3 (mouse) SPSIRCSsVs_____ S423-p
SMAD3 (rat) SPSIRCSsVs_____ S423-p
S425-p
sIRCssVs_______
Upstream
Downstream
32 5
SMAD3 (human)
S425-p
SMAD3 (mouse)
S425-p
SMAD3 (rat)
S425-p
Effects on Modified Protein
  • activity, induced
  • protein conformation
Effects on Biological Processes:
  • cell motility, altered
  • signaling pathway regulation
  • transcription, altered
  • transcription, induced
Regulatory protein:
  • GDF11 (human)
  • HTRA1 (human)
  • MEK1 (human)
  • MSTN (human)
  • MURF1 (human)
  • RHEB (human)
  • SMAD3 (human)
  • SP1 (human)
  • TSC1 (human)
Treatment
  • activin
  • ALK5_Inhibitor_I
  • angiotensin_2
  • anti-CD44
  • cAMP_analog
  • CRM197
  • EGF
  • FGF2
  • gelatin
  • heregulin
  • hyaluronan
  • IGF-1
  • insulin
  • LY2109761
  • LY294002
  • Nodal
  • oncostatin_M
  • rapamycin
  • SB203580
  • SB431542
  • starvation_medium
  • TGF-beta
  • Y27632
SMAD3 (human) sIRCssVs_______ S425-p
SMAD3 (mouse) SIRCSsVs_______ S425-p
SMAD3 (rat) SIRCSsVs_______ S425-p