Other Species / Isoforms mouse rat

p27Kip1 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

S2-gl

______MsNVRVSNG

Upstream

Downstream

1

1

Effects on Modified Protein

intracellular localization
protein degradation

Effects on Biological Processes:

cell cycle regulation
cell growth, induced

Treatment

PUGNAc

p27Kip1 (human)	______MsNVRVSNG	S2-gl
p27Kip1 (mouse)	______MSNVRVsNG	S2
p27Kip1 (rat)	______MSNVRVSNG	S2

S7

_MsNVRVSNGsPsLE

0

2

p27Kip1 (human)	_MsNVRVSNGsPsLE	S7
p27Kip1 (mouse)	_MSNVRVsNGsPsLE	S7-p
p27Kip1 (rat)	_MSNVRVSNGsPSLE	S7

S10-p

NVRVSNGsPsLErMD

Upstream

Downstream

47

37

Effects on Modified Protein

intracellular localization
molecular association, regulation
phosphorylation
protein degradation
protein stabilization

Effects on Biological Processes:

apoptosis, inhibited
carcinogenesis, induced
cell adhesion, induced
cell cycle regulation
cell growth, induced
cell motility, induced
cytoskeletal reorganization
transcription, altered

Kinase, in vitro:

Akt1 (human)
CAMK2A (human)
CDK16 (human)
CDK6 (human)
ERK2 (human)
HIPK2 (human)
KIS (human)
p90RSK (rat)
RSK2 (mouse)

Putative in vivo kinases:

Akt1 (human)
CDK16 (human)
HIPK2 (human)
KIS (human)

Regulatory protein:

DYRK1B (human)
EGLN3 (human)
FBXL12 (human)
MARCKS (human)
MIF4GD (human)

Treatment

A-443654
aflatoxin_B1
antibody
CTGF
cycloheximide
eflornithine
FGF2
H2O2
HGF
hypoxia
KN-93
LY294002
MG132
Nodal
oncostatin_M
oxidative_stress
PD98059
PUGNAc
retinoic_acid
serum
serum_starvation
serum_withdrawal
siRNA
spermidine
tamoxifen
wortmannin

p27Kip1 (human)	NVRVSNGsPsLErMD	S10-p
p27Kip1 (mouse)	NVRVsNGsPsLERMD	S10-p
p27Kip1 (rat)	NVRVSNGsPSLERMD	S10-p

S12-p

RVSNGsPsLErMDAR

Upstream

Downstream

1

6

Effects on Modified Protein

intracellular localization

Effects on Biological Processes:

apoptosis, inhibited
carcinogenesis, induced

Treatment

ischemia

p27Kip1 (human)	RVSNGsPsLErMDAR	S12-p
p27Kip1 (mouse)	RVsNGsPsLERMDAR	S12-p
p27Kip1 (rat)	RVSNGsPSLERMDAR	S12

R15-m1

NGsPsLErMDARQAE

0

1

p27Kip1 (human)	NGsPsLErMDARQAE	R15-m1
p27Kip1 (mouse)	NGsPsLERMDARQAE	R15
p27Kip1 (rat)	NGsPSLERMDARQTE	R15

K25

ARQAEHPKPSACRNL

0

1

p27Kip1 (human)	ARQAEHPKPSACRNL	K25
p27Kip1 (mouse)	ARQAEHPKPSACRNL	K25
p27Kip1 (rat)	ARQTEHPkPSACRNL	K25-ac

K25-ub

ARQAEHPkPSACRNL

0

1

p27Kip1 (human)	ARQAEHPkPSACRNL	K25-ub
p27Kip1 (mouse)	ARQAEHPKPSACRNL	K25
p27Kip1 (rat)	ARQTEHPKPSACRNL	K25

T42

PVDHEELTRDLEKHC

0

1

p27Kip1 (human)	PVDHEELTRDLEKHC	T42
p27Kip1 (mouse)	PVNHEELtRDLEKHC	T42-p
p27Kip1 (rat)	PVNHEELTRDLEKHC	T42

K59

MEEASQRKWNFDFQN

0

1

p27Kip1 (human)	MEEASQRKWNFDFQN	K59
p27Kip1 (mouse)	MEEASQRKWNFDFQN	K59
p27Kip1 (rat)	MEEASQRkWNFDFQN	K59-ac

K68

NFDFQNHKPLEGkyE

0

1

p27Kip1 (human)	NFDFQNHKPLEGkyE	K68
p27Kip1 (mouse)	NFDFQNHKPLEGRYE	K68
p27Kip1 (rat)	NFDFQNHkPLEGRYE	K68-ac

K68-ub

NFDFQNHkPLEGkyE

0

1

p27Kip1 (human)	NFDFQNHkPLEGkyE	K68-ub
p27Kip1 (mouse)	NFDFQNHKPLEGRYE	K68
p27Kip1 (rat)	NFDFQNHKPLEGRYE	K68

Y74-p

HkPLEGkyEWQEVEk

Upstream

Downstream

10

0

Effects on Modified Protein

molecular association, regulation
phosphorylation
protein degradation

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

Src (human)
Yes (human)

Putative in vivo kinases:

Src (human)

Treatment

estradiol
GGTI-2417
PP1

p27Kip1 (human)	HkPLEGkyEWQEVEk	Y74-p
p27Kip1 (mouse)	HKPLEGRYEWQEVER	Y74
p27Kip1 (rat)	HkPLEGRYEWQEVER	Y74

K81-ac

yEWQEVEkGsLPEFy

0

1

p27Kip1 (human)	yEWQEVEkGsLPEFy	K81-ac
p27Kip1 (mouse)	YEWQEVERGsLPEFy	R81
p27Kip1 (rat)	YEWQEVERGSLPEFY	R81

K81-ub

yEWQEVEkGsLPEFy

0

1

p27Kip1 (human)	yEWQEVEkGsLPEFy	K81-ub
p27Kip1 (mouse)	YEWQEVERGsLPEFy	R81
p27Kip1 (rat)	YEWQEVERGSLPEFY	R81

S83-p

WQEVEkGsLPEFyyR

Upstream

Downstream

2

0

Effects on Modified Protein

protein conformation

Kinase, in vitro:

CK2A1 (human)

p27Kip1 (human)	WQEVEkGsLPEFyyR	S83-p
p27Kip1 (mouse)	WQEVERGsLPEFyYR	S83-p
p27Kip1 (rat)	WQEVERGSLPEFYYR	S83

Y88-p

kGsLPEFyyRPPRPP

Upstream

Downstream

14

0

Effects on Modified Protein

activity, inhibited
intracellular localization
molecular association, regulation
phosphorylation
protein conformation
protein degradation
ubiquitination

Effects on Biological Processes:

carcinogenesis, induced
cell cycle regulation
cell growth, induced

Kinase, in vitro:

Abl (human)
Abl (mouse)
LYN (mouse)
Src (human)
Yes (human)

Putative in vivo kinases:

Abl (human)
Abl (mouse)
Ack (human)
LYN (human)
Src (human)

Treatment

(R)-9bMS
dasatinib
Epo
GGTI-2417
imatinib
JAK_inhibitor_I
siRNA

p27Kip1 (human)	kGsLPEFyyRPPRPP	Y88-p
p27Kip1 (mouse)	RGsLPEFyYRPPRPP	Y88-p
p27Kip1 (rat)	RGSLPEFYYRPPRPP	Y88

Y89-p

GsLPEFyyRPPRPPK

Upstream

Downstream

8

0

Effects on Modified Protein

activity, inhibited
intracellular localization
molecular association, regulation

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

Abl (human)
Src (human)
Yes (human)

Putative in vivo kinases:

Src (human)

p27Kip1 (human)	GsLPEFyyRPPRPPK	Y89-p
p27Kip1 (mouse)	GsLPEFyYRPPRPPK	Y89
p27Kip1 (rat)	GSLPEFYYRPPRPPK	Y89

K100

RPPKGACKVPAQEsQ

0

1

p27Kip1 (human)	RPPKGACKVPAQEsQ	K100
p27Kip1 (mouse)	RPPKSACKVLAQEsQ	K100
p27Kip1 (rat)	RPPKSACkVPAQEsL	K100-ac

S106

CKVPAQESQDVsGSR

0

13

p27Kip1 (human)	CKVPAQESQDVsGSR	S106
p27Kip1 (mouse)	CKVLAQEsQDVSGSR	S106-p
p27Kip1 (rat)	CkVPAQEsLDVSGSR	S106-p

S106-gl

CKVPAQEsQDVsGSR

1

1

p27Kip1 (human)	CKVPAQEsQDVsGSR	S106-gl
p27Kip1 (mouse)	CKVLAQESQDVSGSR	S106
p27Kip1 (rat)	CkVPAQESLDVSGSR	S106

S110-gl

AQEsQDVsGSRPAAP

1

1

p27Kip1 (human)	AQEsQDVsGSRPAAP	S110-gl
p27Kip1 (mouse)	AQEsQDVSGSRQAVP	S110
p27Kip1 (rat)	AQEsLDVSGSRQAVP	S110

K134-ub

DTHLVDPkTDPSDsQ

Upstream

Downstream

2

0

Effects on Modified Protein

protein degradation

Effects on Biological Processes:

cell cycle regulation

Regulatory protein:

WNT10B (human)

p27Kip1 (human)	DTHLVDPkTDPSDsQ	K134-ub
p27Kip1 (mouse)	DRHLVDQMPDSSDNP	M134
p27Kip1 (rat)	DRHLVDQMPDSSDSP	M134

S140-p

PkTDPSDsQTGLAEQ

Upstream

Downstream

3

5

Effects on Modified Protein

protein stabilization

Effects on Biological Processes:

cell cycle regulation
cell growth, inhibited

Kinase, in vitro:

ATM (human)
DNAPK (human)

Phosphatases, in vitro:

PPM1D (human)

Putative in vivo kinases:

ATM (human)

Putative upstream phosphatases:

PPM1D (human)

Treatment

GSK2830371
H2O2
ionizing_radiation
ischemia
KU-55933

p27Kip1 (human)	PkTDPSDsQTGLAEQ	S140-p
p27Kip1 (mouse)	QMPDSSDNPAGLAEQ	N140
p27Kip1 (rat)	QMPDSSDSPAGLAEQ	S140

K153-ub

EQCAGIRkrPAtDDS

Upstream

Downstream

2

1

Effects on Modified Protein

protein degradation

Effects on Biological Processes:

cell cycle regulation

Regulatory protein:

WNT10B (human)

p27Kip1 (human)	EQCAGIRkrPAtDDS	K153-ub
p27Kip1 (mouse)	EQCPGMRKRPAAEDS	K153
p27Kip1 (rat)	EQCPGMRKRPAAEDS	K153

R154-m2

QCAGIRkrPAtDDSS

0

1

p27Kip1 (human)	QCAGIRkrPAtDDSS	R154-m2
p27Kip1 (mouse)	QCPGMRKRPAAEDSS	R154
p27Kip1 (rat)	QCPGMRKRPAAEDSS	R154

T157-p

GIRkrPAtDDSSTQN

Upstream

Downstream

25

1

Effects on Modified Protein

intracellular localization
molecular association, regulation
phosphorylation
protein degradation

Effects on Biological Processes:

apoptosis, inhibited
cell cycle regulation
cell growth, altered
cell motility, induced
cytoskeletal reorganization
transcription, induced

Kinase, in vitro:

Akt1 (human)
CAMK1A (human)
Pim1 (human)
SGK1 (human)

Phosphatases, in vitro:

PPP2R5C (human)

Putative in vivo kinases:

Akt1 (human)
CAMK1A (human)
Pim1 (human)
SGK1 (human)

Putative upstream phosphatases:

PPP2R5C (human)

Regulatory protein:

Akt1 (human)
Akt2 (human)
Akt3 (human)
EGLN3 (human)
FBXL12 (human)
PCGF2 (human)
PHLPP (human)
PHLPP2 (human)
Raptor (human)
RICTOR (human)
SGK1 (human)
TSC2 (human)

Treatment

Akt_inhibitor_VIII
amino_acids
androstanolone
CTGF
estradiol
hypoxia
insulin
interleukin-6-hydroxymethyl-chiro-inositol-2(R)-2-methyl-3-O-octadecylcarbonate
KN-93
leptomycin_B
LY294002
Nodal
PD98059
rapamycin
serum
siRNA
TGF-beta
U0126
wortmannin

p27Kip1 (human)	GIRkrPAtDDSSTQN	T157-p
p27Kip1 (mouse)	GMRKRPAAEDSSSQN	A157
p27Kip1 (rat)	GMRKRPAAEDSSSQN	A157

T157-gl

GIRkrPAtDDSSTQN

1

1

p27Kip1 (human)	GIRkrPAtDDSSTQN	T157-gl
p27Kip1 (mouse)	GMRKRPAAEDSSSQN	A157
p27Kip1 (rat)	GMRKRPAAEDSSSQN	A157

K165-ub

DDSSTQNkRANRTEE

Upstream

Downstream

3

0

Effects on Modified Protein

protein degradation
ubiquitination

Effects on Biological Processes:

cell cycle regulation
cell growth, induced

Regulatory protein:

WNT10B (human)

p27Kip1 (human)	DDSSTQNkRANRTEE	K165-ub
p27Kip1 (mouse)	EDSSSQNkRANRtEE	K165-ub
p27Kip1 (rat)	EDSSSQNKRANRTEE	K165

T170

QNkRANRTEENVsDG

2

0

p27Kip1 (human)	QNkRANRTEENVsDG	T170
p27Kip1 (mouse)	QNkRANRtEENVsDG	T170-p
p27Kip1 (rat)	QNKRANRTEENVSDG	T170

S175-p

NRTEENVsDGsPNAG

1

3

p27Kip1 (human)	NRTEENVsDGsPNAG	S175-p
p27Kip1 (mouse)	NRtEENVsDGsPNAG	S175-p
p27Kip1 (rat)	NRTEENVSDGsPNAG	S175

S178-p

EENVsDGsPNAGsVE

Upstream

4

14

Kinase, in vitro:

ERK2 (human)

Treatment

LY294002

p27Kip1 (human)	EENVsDGsPNAGsVE	S178-p
p27Kip1 (mouse)	EENVsDGsPNAGtVE	S178-p
p27Kip1 (rat)	EENVSDGsPNAGTVE	S178-p

S183-p

DGsPNAGsVEQtPKK

1

2

p27Kip1 (human)	DGsPNAGsVEQtPKK	S183-p
p27Kip1 (mouse)	DGsPNAGtVEQtPKK	T183-p
p27Kip1 (rat)	DGsPNAGTVEQTPKK	T183

T187-p

NAGsVEQtPKKPGLR

Upstream

Downstream

53

1

Effects on Modified Protein

molecular association, regulation
protein conformation
protein degradation
protein stabilization
ubiquitination

Effects on Biological Processes:

apoptosis, induced
carcinogenesis, induced
carcinogenesis, inhibited
cell cycle regulation
cell growth, induced
cell growth, inhibited

Kinase, in vitro:

CDK1 (human)
CDK2 (human)
CDK2 (mouse)
CDK6 (human)
ERK1 (human)
ERK2 (human)

Putative in vivo kinases:

CDK2 (human)

Regulatory protein:

CAMK2N1 (human)
CCNE1 (human)
CDK5 (human)
EGLN3 (human)
MARCKS (human)
MIF4GD (human)
PFKFB3 (human)
SIRT1 (human)
SKP2 (human)
TRIM21 (human)
WISP2 (human)

Treatment

A-443654
adriamycin
antibody
eflornithine
F2,6BP
FGF2
GGTI-2417
hypoxia
KN-92
LY294002
melphalan
MG132
Nodal
phorbol_ester
retinoic_acid
serum
siRNA
spermidine
U0126

p27Kip1 (human)	NAGsVEQtPKKPGLR	T187-p
p27Kip1 (mouse)	NAGtVEQtPKKPGLR	T187-p
p27Kip1 (rat)	NAGTVEQTPKKPGLR	T187

T198-p

PGLRRRQt_______

Upstream

Downstream

27

6

Effects on Modified Protein

intracellular localization
molecular association, regulation
protein degradation
protein stabilization
ubiquitination

Effects on Biological Processes:

apoptosis, induced
carcinogenesis, inhibited
cell cycle regulation
cell growth, altered
cell motility, induced
transcription, induced

Kinase, in vitro:

Akt1 (human)
AMPKA1 (human)
CAMK1A (human)
p90RSK (rat)
Pim1 (human)
RSK2 (mouse)
SGK1 (human)

Putative in vivo kinases:

Akt1 (human)
CAMK1A (human)
p90RSK (rat)
Pim1 (human)
RSK2 (mouse)

Regulatory protein:

DYRK1B (human)
EGLN3 (human)
FBXL12 (human)
LKB1 (human)
MIF4GD (human)

Treatment

A-443654
aflatoxin_B1
AZD1208
chrysin
eflornithine
glucose_starvation
hypoxia
KN-93
LY294002
MG132
Nodal
serum
spermidine

p27Kip1 (human)	PGLRRRQt_______	T198-p
p27Kip1 (mouse)	KPGLRRQt_______	T197-p
p27Kip1 (rat)	KPGLRRQT_______	T197

T198-gl

PGLRRRQt_______

1

1

p27Kip1 (human)	PGLRRRQt_______	T198-gl
p27Kip1 (mouse)	KPGLRRQT_______	T197
p27Kip1 (rat)	KPGLRRQT_______	T197

K73-sm

NHkPLEGkyEWQEVE

Downstream

1

0

Effects on Modified Protein

intracellular localization

Effects on Biological Processes:

carcinogenesis, induced
cell growth, induced

p27Kip1 (human)	NHkPLEGkyEWQEVE	K73-sm
p27Kip1 (mouse)	NHKPLEGRYEWQEVE	R73
p27Kip1 (rat)	NHkPLEGRYEWQEVE	R73