p21Cip1 (human)

LTP

LTP (Low Throughput Papers) : The number of records in which this modification site was determined using methods other than discovery mass spectrometry.

HTP

HTP (High Throughput Papers): The number of records in which this modification site was assigned using ONLY proteomic discovery mass spectrometry.

______MSEPAGDVR

p21Cip1 (human)	______MSEPAGDVR	S2
p21Cip1 (mouse)	______MsNPGDVRP	S2-p
p21Cip1 (rat)	______MSDPGDVRP	S2
p21Cip1 (dog)	gap	-

K16-ub

RQNPCGSkACRRLFG

Upstream

Downstream

Effects on Modified Protein

protein degradation
ubiquitination

Effects on Biological Processes:

carcinogenesis, induced
cell cycle regulation
cell growth, induced

Treatment

311
IL-1a

p21Cip1 (human)	RQNPCGSkACRRLFG	K16-ub
p21Cip1 (mouse)	RPVPHRSKVCRCLFG	K15
p21Cip1 (rat)	RPVPHRSKVCRRLFG	K15
p21Cip1 (dog)	gap	-

S31-p

PVDSEQLsRDCDALM

p21Cip1 (human)	PVDSEQLsRDCDALM	S31-p
p21Cip1 (mouse)	PVDSEQLRRDCDALM	R30
p21Cip1 (rat)	PVDSEQLSRDCDALM	S30
p21Cip1 (dog)	gap	-

T55-p

RWNFDFVtEtPLEGD

Upstream

Downstream

Effects on Modified Protein

activity, induced
intracellular localization
molecular association, regulation
protein stabilization

Effects on Biological Processes:

apoptosis, induced
cell cycle regulation
cell differentiation, inhibited
cell growth, inhibited
signaling pathway regulation

Kinase, in vitro:

MELK (mouse)

Putative in vivo kinases:

MELK (mouse)

p21Cip1 (human)	RWNFDFVtEtPLEGD	T55-p
p21Cip1 (mouse)	RWNFDFVTETPLEGN	T54
p21Cip1 (rat)	RWNFDFATETPLEGN	T54
p21Cip1 (dog)	RWNFDFVTETPLEGD	T23

T57-p

NFDFVtEtPLEGDFA

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation
phosphorylation
protein degradation
protein stabilization
ubiquitination

Effects on Biological Processes:

apoptosis, induced
cell cycle regulation

Kinase, in vitro:

ERK2 (human)
GSK3B (human)
JNK1 (human)
JNK2 (human)
P38A (human)

Putative in vivo kinases:

ERK2 (human)
GSK3A (human)

Regulatory protein:

MST1 (mouse)
MST2 (mouse)

Treatment

botulinum_C3_toxin
EGF
U0126

p21Cip1 (human)	NFDFVtEtPLEGDFA	T57-p
p21Cip1 (mouse)	NFDFVTETPLEGNFV	T56
p21Cip1 (rat)	NFDFATETPLEGNYV	T56
p21Cip1 (dog)	NFDFVTETPLEGDFA	T25

K75-ub

VRGLGLPkLYLPtGP

Upstream

Downstream

Effects on Modified Protein

protein degradation
ubiquitination

Treatment

311
IL-1a

p21Cip1 (human)	VRGLGLPkLYLPtGP	K75-ub
p21Cip1 (mouse)	VRSLGLPKVyLsPGS	K74
p21Cip1 (rat)	VRSPGLPKIYLSPGS	K74
p21Cip1 (dog)	VRGLGLSKVSLPAGP	K43

Y77

GLGLPkLYLPtGPRR

p21Cip1 (human)	GLGLPkLYLPtGPRR	Y77
p21Cip1 (mouse)	SLGLPKVyLsPGSRS	Y76-p
p21Cip1 (rat)	SPGLPKIYLSPGSRR	Y76
p21Cip1 (dog)	GLGLSKVSLPAGPRG	S45

P79

GLPkLYLPtGPRRGR

p21Cip1 (human)	GLPkLYLPtGPRRGR	P79
p21Cip1 (mouse)	GLPKVyLsPGSRSRD	S78-p
p21Cip1 (rat)	GLPKIYLSPGSRRRD	S78
p21Cip1 (dog)	GLSKVSLPAGPRGGR	P47

T80-p

LPkLYLPtGPRRGRD

Upstream

Kinase, in vitro:

LKB1 (human)

Putative in vivo kinases:

LKB1 (human)

p21Cip1 (human)	LPkLYLPtGPRRGRD	T80-p
p21Cip1 (mouse)	LPKVyLsPGSRSRDD	P79
p21Cip1 (rat)	LPKIYLSPGSRRRDD	P79
p21Cip1 (dog)	LSKVSLPAGPRGGRD	A48

S98-p

GGRRPGTsPALLQGT

Upstream

Kinase, in vitro:

ASK1 (human)
CDK4 (human)
ERK1 (human)
ERK2 (human)
JNK1 (human)
JNK2 (human)
JNK3 (human)
P38A (human)

Putative in vivo kinases:

ASK1 (human)

Regulatory protein:

CDK4 (human)

Treatment

1NM-PP1
PD0332991
serum

p21Cip1 (human)	GGRRPGTsPALLQGT	S98-p
p21Cip1 (mouse)	GDKRPSTSSALLQGP	S96
p21Cip1 (rat)	GDKRPSTSSALLQGP	S96
p21Cip1 (dog)	GGKRPGTSPALLQGT	S66

S114-p

EEDHVDLsLSCTLVP

Upstream

Downstream

Effects on Modified Protein

protein degradation

Kinase, in vitro:

GSK3B (human)

Putative in vivo kinases:

GSK3B (human)

Treatment

caffeine
lithium
siRNA
TDZD-8
UV

p21Cip1 (human)	EEDHVDLsLSCTLVP	S114-p
p21Cip1 (mouse)	PEDHVALSLSCTLVS	S112
p21Cip1 (rat)	PEDHVALSLSCTLVS	S114
p21Cip1 (dog)	QEDHLDLSLTCTLLP	S82

S123-p

SCTLVPRsGEQAEGs

p21Cip1 (human)	SCTLVPRsGEQAEGs	S123-p
p21Cip1 (mouse)	gap	-
p21Cip1 (rat)	SCTLVSHAPERPEDS	A123
p21Cip1 (dog)	TCTLLPHsPERPEAS	S91-p

S130-p

sGEQAEGsPGGPGDs

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation
phosphorylation
protein degradation
protein stabilization
ubiquitination

Effects on Biological Processes:

cell cycle regulation

Kinase, in vitro:

CDK1 (human)
CDK2 (human)
CDK4 (human)
CDK6 (human)
ERK1 (human)
ERK2 (human)
JNK1 (human)
JNK2 (human)
JNK3 (human)
P38A (human)

Putative in vivo kinases:

CDK1 (human)
CDK2 (human)
ERK2 (human)
JNK1 (human)
P38A (human)
P38A (mouse)

Regulatory protein:

CDK4 (human)
CDK6 (human)

Treatment

1NM-PP1
EGF
MG132
neferine
PD0332991
SB203580
seliciclib
serum
siRNA
SP600125
TGF-beta
U0126

p21Cip1 (human)	sGEQAEGsPGGPGDs	S130-p
p21Cip1 (mouse)	VSERPEDSPGGPGTS	S125
p21Cip1 (rat)	APERPEDSPGGTGTS	S130
p21Cip1 (dog)	sPERPEASPGVPGTS	S98

S137-p

sPGGPGDsQGRkRRQ

p21Cip1 (human)	sPGGPGDsQGRkRRQ	S137-p
p21Cip1 (mouse)	SPGGPGTSQGRKRRQ	S132
p21Cip1 (rat)	SPGGTGTSQGRKRRQ	S137
p21Cip1 (dog)	SPGVPGTSQGRKRRQ	S105

K141-ub

PGDsQGRkRRQtsMT

Upstream

Downstream

Effects on Modified Protein

protein degradation
ubiquitination

Treatment

311
IL-1a

p21Cip1 (human)	PGDsQGRkRRQtsMT	K141-ub
p21Cip1 (mouse)	PGTSQGRKRRQtsLT	K136
p21Cip1 (rat)	TGTSQGRKRRQtsLT	K141
p21Cip1 (dog)	PGTSQGRKRRQTSMT	K109

K141-ac

PGDsQGRkRRQtsMT

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein stabilization

Effects on Biological Processes:

carcinogenesis, inhibited
cell cycle regulation
cell growth, inhibited

Treatment

siRNA
trichostatin_A

p21Cip1 (human)	PGDsQGRkRRQtsMT	K141-ac
p21Cip1 (mouse)	PGTSQGRKRRQtsLT	K136
p21Cip1 (rat)	TGTSQGRKRRQtsLT	K141
p21Cip1 (dog)	PGTSQGRKRRQTSMT	K109

T145-p

QGRkRRQtsMTDFyH

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation
phosphorylation
protein degradation
protein stabilization

Effects on Biological Processes:

carcinogenesis, induced
cell cycle regulation
cell growth, induced
cell growth, inhibited
cell motility, induced

Kinase, in vitro:

Akt1 (human)
DAPK3 (human)
Pim1 (human)
Pim2 (human)

Putative in vivo kinases:

Akt1 (human)
DAPK3 (human)
Pim1 (human)
Pim2 (human)

Regulatory protein:

CTNND1 (human)
Pim1 (human)

Treatment

EGF
gefitinib
insulin
LY294002
serum
silmitasertib
taxol
wortmannin

p21Cip1 (human)	QGRkRRQtsMTDFyH	T145-p
p21Cip1 (mouse)	QGRKRRQtsLTDFYH	T140-p
p21Cip1 (rat)	QGRKRRQtsLTDFYH	T145-p
p21Cip1 (dog)	QGRKRRQTSMTDFYH	T113

S146-p

GRkRRQtsMTDFyHs

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation
protein degradation
protein stabilization

Effects on Biological Processes:

apoptosis, induced
cell growth, altered

Kinase, in vitro:

Akt1 (human)
LATS2 (human)
NuaK1 (human)
Pim1 (human)
Pim2 (human)
PKCA (human)

Putative in vivo kinases:

Akt1 (human)
LATS2 (human)

Regulatory protein:

Pim1 (human)

Treatment

LY294002
okadaic_acid
serum
siRNA
taxol
UV
wortmannin

p21Cip1 (human)	GRkRRQtsMTDFyHs	S146-p
p21Cip1 (mouse)	GRKRRQtsLTDFYHS	S141-p
p21Cip1 (rat)	GRKRRQtsLTDFYHS	S146-p
p21Cip1 (dog)	GRKRRQTSMTDFYHS	S114

Y151-p

QtsMTDFyHskRrLI

p21Cip1 (human)	QtsMTDFyHskRrLI	Y151-p
p21Cip1 (mouse)	QtsLTDFYHSKRRLV	Y146
p21Cip1 (rat)	QtsLTDFYHSKRRLV	Y151
p21Cip1 (dog)	QTSMTDFYHSKRRLI	Y119

S153-p

sMTDFyHskRrLIFS

Upstream

Downstream

Effects on Modified Protein

intracellular localization
molecular association, regulation

Kinase, in vitro:

DYRK1B (human)
PKCA (human)

Putative in vivo kinases:

DYRK1B (human)
PKCA (human)

Treatment

phorbol_ester
SB203580

p21Cip1 (human)	sMTDFyHskRrLIFS	S153-p
p21Cip1 (mouse)	sLTDFYHSKRRLVFC	S148
p21Cip1 (rat)	sLTDFYHSKRRLVFC	S153
p21Cip1 (dog)	SMTDFYHSKRRLIFS	S121

K154-ub

MTDFyHskRrLIFSk

Upstream

Downstream

Effects on Modified Protein

protein degradation
ubiquitination

Effects on Biological Processes:

carcinogenesis, induced
cell cycle regulation
cell growth, induced

Treatment

311
IL-1a

p21Cip1 (human)	MTDFyHskRrLIFSk	K154-ub
p21Cip1 (mouse)	LTDFYHSKRRLVFCK	K149
p21Cip1 (rat)	LTDFYHSKRRLVFCK	K154
p21Cip1 (dog)	MTDFYHSKRRLIFSK	K122

K154-ac

MTDFyHskRrLIFSk

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein stabilization

Effects on Biological Processes:

carcinogenesis, inhibited
cell cycle regulation
cell growth, inhibited

Treatment

siRNA
trichostatin_A

p21Cip1 (human)	MTDFyHskRrLIFSk	K154-ac
p21Cip1 (mouse)	LTDFYHSKRRLVFCK	K149
p21Cip1 (rat)	LTDFYHSKRRLVFCK	K154
p21Cip1 (dog)	MTDFYHSKRRLIFSK	K122

R156-m2

DFyHskRrLIFSkRk

Downstream

Effects on Modified Protein

intracellular localization

p21Cip1 (human)	DFyHskRrLIFSkRk	R156-m2
p21Cip1 (mouse)	DFYHSKRRLVFCKRK	R151
p21Cip1 (rat)	DFYHSKRRLVFCKRK	R156
p21Cip1 (dog)	DFYHSKRRLIFSKRK	R124

K161-ub

kRrLIFSkRkP____

Upstream

Downstream

Effects on Modified Protein

protein degradation
ubiquitination

Effects on Biological Processes:

apoptosis, inhibited
carcinogenesis, induced
cell cycle regulation
cell growth, induced

Treatment

311
IL-1a

p21Cip1 (human)	kRrLIFSkRkP____	K161-ub
p21Cip1 (mouse)	KRRLVFCKRKP____	K156
p21Cip1 (rat)	KRRLVFCKRKP____	K161
p21Cip1 (dog)	KRRLIFSKRKP____	K129

K161-ac

kRrLIFSkRkP____

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein stabilization

Effects on Biological Processes:

carcinogenesis, inhibited
cell cycle regulation
cell growth, inhibited

Treatment

siRNA
trichostatin_A

p21Cip1 (human)	kRrLIFSkRkP____	K161-ac
p21Cip1 (mouse)	KRRLVFCKRKP____	K156
p21Cip1 (rat)	KRRLVFCKRKP____	K161
p21Cip1 (dog)	KRRLIFSKRKP____	K129

K163-ub

rLIFSkRkP______

Upstream

Downstream

Effects on Modified Protein

protein degradation
ubiquitination

Effects on Biological Processes:

carcinogenesis, induced
cell cycle regulation
cell growth, induced

Treatment

311
IL-1a

p21Cip1 (human)	rLIFSkRkP______	K163-ub
p21Cip1 (mouse)	RLVFCKRKP______	K158
p21Cip1 (rat)	RLVFCKRKP______	K163
p21Cip1 (dog)	RLIFSKRKP______	K131

K163-ac

rLIFSkRkP______

Upstream

Downstream

Effects on Modified Protein

molecular association, regulation
protein stabilization

Effects on Biological Processes:

carcinogenesis, inhibited
cell cycle regulation
cell growth, inhibited

Treatment

siRNA
trichostatin_A

p21Cip1 (human)	rLIFSkRkP______	K163-ac
p21Cip1 (mouse)	RLVFCKRKP______	K158
p21Cip1 (rat)	RLVFCKRKP______	K163
p21Cip1 (dog)	RLIFSKRKP______	K131