Ser778
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Home > Phosphorylation Site Page: > Ser778  -  DYN1 (rat)

Site Information
GRRsPTSsPTPQRRA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450206

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 2 , 5 , 7 ) , immunoprecipitation ( 4 , 7 ) , mass spectrometry ( 2 , 6 ) , mutation of modification site ( 1 , 5 ) , phospho-antibody ( 3 , 5 , 7 ) , western blotting ( 2 , 3 , 4 )
Relevant cell line - cell type - tissue:
'neuron, cerebellar granule' ( 3 ) , 'neuron, cerebellar granule'-brain ( 5 ) , B104 (neuron) ( 7 ) , brain ( 2 , 4 ) , E.coli (bacterial) ( 1 ) , neuron:synaptosome ( 2 ) , neuron:synaptosome-'brain, cerebral cortex' ( 7 ) , neuron:synaptosome-brain ( 5 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (rat) ( 3 , 7 )
Kinases, in vitro:
CDK5 (human) ( 3 ) , CDK5 (rat) ( 7 )
Treatments:
AR-A014418 ( 3 ) , CT-99021 ( 3 ) , depolarization ( 2 ) , PD98059 ( 7 ) , seliciclib ( 3 , 7 )

Downstream Regulation
Effects of modification on DYN1:
molecular association, regulation ( 4 , 5 )
Effects of modification on biological processes:
endocytosis, altered ( 4 )
Induce interaction with:
PACSIN1 (rat) ( 4 ) , endophilin A1 (human) ( 4 )
Inhibit interaction with:
PACSIN1 (rat) ( 5 )

References 

1

Xue J, et al. (2011) Calcineurin Selectively Docks with the Dynamin Ixb Splice Variant to Regulate Activity-dependent Bulk Endocytosis. J Biol Chem 286, 30295-303
21730063   Curated Info

2

Chan LS, Hansra G, Robinson PJ, Graham ME (2010) Differential phosphorylation of dynamin I isoforms in subcellular compartments demonstrates the hidden complexity of phosphoproteomes. J Proteome Res 9, 4028-37
20560669   Curated Info

3

Clayton EL, et al. (2010) Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles. Nat Neurosci 13, 845-51
20526333   Curated Info

4

Anggono V, Robinson PJ (2007) Syndapin I and endophilin I bind overlapping proline-rich regions of dynamin I: role in synaptic vesicle endocytosis. J Neurochem 102, 931-43
17437541   Curated Info

5

Anggono V, et al. (2006) Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis. Nat Neurosci 9, 752-60
16648848   Curated Info

6

Larsen MR, Graham ME, Robinson PJ, Roepstorff P (2004) Improved detection of hydrophilic phosphopeptides using graphite powder microcolumns and mass spectrometry: evidence for in vivo doubly phosphorylated dynamin I and dynamin III. Mol Cell Proteomics 3, 456-65
14762214   Curated Info

7

Tan TC, et al. (2003) Cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5, 701-10
12855954   Curated Info