Ser774
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Home > Phosphorylation Site Page: > Ser774  -  DYN1 (rat)

Site Information
SVPAGRRsPTSsPTP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450205

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 , 7 , 9 ) , immunoprecipitation ( 6 , 9 ) , mass spectrometry ( 2 , 4 , 8 ) , mutation of modification site ( 3 , 7 ) , phospho-antibody ( 1 , 5 , 7 , 9 ) , western blotting ( 1 , 4 , 5 , 6 )
Relevant cell line - cell type - tissue:
'neuron, cerebellar granule' ( 5 ) , 'neuron, cerebellar granule'-brain ( 1 , 7 ) , adipose tissue ( 2 ) , B104 (neuron) ( 9 ) , brain ( 4 , 6 ) , E.coli (bacterial) ( 3 ) , neuron:synaptosome ( 4 ) , neuron:synaptosome-'brain, cerebral cortex' ( 9 ) , neuron:synaptosome-brain ( 7 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (rat) ( 9 ) , GSK3B (human) ( 5 )
Kinases, in vitro:
CDK5 (rat) ( 9 ) , GSK3B (human) ( 5 )
Treatments:
action_potential ( 1 ) , Akt-I-1,2 ( 1 ) , Akt_inhibitor_X ( 1 ) , APV ( 1 ) , AR-A014418 ( 5 ) , CNQX ( 1 ) , CT-99021 ( 5 ) , depolarization ( 4 ) , PD98059 ( 9 ) , seliciclib ( 5 , 9 )

Downstream Regulation
Effects of modification on DYN1:
molecular association, regulation ( 6 , 7 )
Effects of modification on biological processes:
endocytosis, altered ( 5 , 6 )
Induce interaction with:
PACSIN1 (rat) ( 6 ) , endophilin A1 (human) ( 6 )
Inhibit interaction with:
PACSIN1 (rat) ( 7 )

References 

1

Smillie KJ, Cousin MA (2012) Akt/PKB controls the activity-dependent bulk endocytosis of synaptic vesicles. Traffic 13, 1004-11
22487004   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Xue J, et al. (2011) Calcineurin Selectively Docks with the Dynamin Ixb Splice Variant to Regulate Activity-dependent Bulk Endocytosis. J Biol Chem 286, 30295-303
21730063   Curated Info

4

Chan LS, Hansra G, Robinson PJ, Graham ME (2010) Differential phosphorylation of dynamin I isoforms in subcellular compartments demonstrates the hidden complexity of phosphoproteomes. J Proteome Res 9, 4028-37
20560669   Curated Info

5

Clayton EL, et al. (2010) Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles. Nat Neurosci 13, 845-51
20526333   Curated Info

6

Anggono V, Robinson PJ (2007) Syndapin I and endophilin I bind overlapping proline-rich regions of dynamin I: role in synaptic vesicle endocytosis. J Neurochem 102, 931-43
17437541   Curated Info

7

Anggono V, et al. (2006) Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis. Nat Neurosci 9, 752-60
16648848   Curated Info

8

Larsen MR, Graham ME, Robinson PJ, Roepstorff P (2004) Improved detection of hydrophilic phosphopeptides using graphite powder microcolumns and mass spectrometry: evidence for in vivo doubly phosphorylated dynamin I and dynamin III. Mol Cell Proteomics 3, 456-65
14762214   Curated Info

9

Tan TC, et al. (2003) Cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5, 701-10
12855954   Curated Info