Ser65

Site Information
DyNIQkEstLHLVLr SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 9765474

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 2 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Disease tissue studied:	bone cancer ( 2 ) , breast cancer ( 5 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 ) , lung cancer ( 5 ) , non-small cell lung cancer ( 5 )
Relevant cell line - cell type - tissue:	breast ( 3 ) , BT-20 (breast cell) ( 5 ) , H2009 (pulmonary) ( 5 ) , H2887 (pulmonary) ( 5 ) , HCC1359 (pulmonary) ( 5 ) , HCC1937 (breast cell) ( 5 ) , HCC2279 (pulmonary) ( 5 ) , HCC366 (pulmonary) ( 5 ) , HCC4006 (pulmonary) ( 5 ) , HCC78 (pulmonary) ( 5 ) , HeLa (cervical) ( 6 ) , HOP62 (pulmonary) ( 5 ) , K562 (erythroid) ( 4 ) , MCF-7 (breast cell) ( 5 ) , MDA-MB-468 (breast cell) ( 5 ) , NCI-H2172 (pulmonary) ( 5 ) , NCI-H322 (pulmonary) ( 5 ) , U2OS (bone cell) ( 2 )

Upstream Regulation
Regulatory protein:	PARK2 (human) ( 2 ) , PINK1 (human) ( 2 ) , USP2 (human) ( 2 )
Treatments:	BI2536 ( 2 ) , CCCP ( 2 ) , colcemid ( 2 )

References
1	Zhang CL, et al. (2022) The retracted conformation of ubiquitin Ser65 phosphorylation inhibits the formation of K48-linked ubiquitin chains. Biosci Rep 36205098 Curated Info
2	Mann G, et al. (2022) Antibody for Serine 65 Phosphorylated Ubiquitin Identifies PLK1-Mediated Phosphorylation of Mitotic Proteins and APC1. Molecules 27 35956818 Curated Info
3	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
4	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
5	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
6	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info