Ser81
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Home > Phosphorylation Site Page: > Ser81  -  PR (human)

Site Information
TQDQQsLsDVEGAys   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450160

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 , 9 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 5 ) , peptide sequencing ( 9 ) , phospho-antibody ( 4 , 5 ) , phosphopeptide mapping ( 7 , 9 ) , western blotting ( 4 , 5 )
Disease tissue studied:
breast cancer ( 2 , 3 , 4 , 5 , 7 , 9 ) , breast ductal carcinoma ( 3 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 )
Relevant cell line - cell type - tissue:
breast ( 1 , 3 ) , BT-474 (breast cell) ( 2 ) , COS (fibroblast) ( 4 ) , HeLa (cervical) ( 4 , 5 ) , SF9 ( 7 ) , T47D (breast cell) ( 4 , 5 , 6 , 7 , 9 )

Upstream Regulation
Regulatory protein:
DUSP6 (human) ( 4 )
Putative in vivo kinases:
CK2A1 (human) ( 5 )
Kinases, in vitro:
CK2A1 (human) ( 9 )
Treatments:
DMAT ( 5 ) , EGF ( 5 ) , mifepristone ( 5 ) , mimosine ( 5 ) , PP2 ( 5 ) , progestin_R5020 ( 4 , 5 ) , seliciclib ( 5 ) , serum ( 5 ) , TBB ( 5 ) , U0126 ( 5 )

Downstream Regulation
Effects of modification on PR:
molecular association, regulation ( 4 , 5 )
Effects of modification on biological processes:
carcinogenesis, induced ( 5 ) , cell growth, induced ( 5 ) , transcription, induced ( 4 , 5 , 8 )
Induce interaction with:
DNA ( 4 , 5 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Hagan CR, Knutson TP, Lange CA (2013) A Common Docking Domain in Progesterone Receptor-B links DUSP6 and CK2 signaling to proliferative transcriptional programs in breast cancer cells. Nucleic Acids Res 41, 8926-42
23921636   Curated Info

5

Hagan CR, Regan TM, Dressing GE, Lange CA (2011) ck2-dependent phosphorylation of progesterone receptors (PR) on Ser81 regulates PR-B isoform-specific target gene expression in breast cancer cells. Mol Cell Biol 31, 2439-52
21518957   Curated Info

6

Knotts TA, et al. (2001) Identification of a phosphorylation site in the hinge region of the human progesterone receptor and additional amino-terminal phosphorylation sites. J Biol Chem 276, 8475-83
11110801   Curated Info

7

Beck CA, et al. (1996) Stoichiometry and site-specific phosphorylation of human progesterone receptor in native target cells and in the baculovirus expression system. J Biol Chem 271, 19546-55
8702648   Curated Info

8

Takimoto GS, et al. (1996) Role of phosphorylation on DNA binding and transcriptional functions of human progesterone receptors. J Biol Chem 271, 13308-16
8662865   Curated Info

9

Zhang Y, et al. (1994) Identification of phosphorylation sites unique to the B form of human progesterone receptor. In vitro phosphorylation by casein kinase II. J Biol Chem 269, 31034-40
7983041   Curated Info

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