Thr80

Site Information
GFINRNDtkEDVFVH SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 9412957

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 3 , 4 ) , mass spectrometry (in vitro) ( 2 )
Disease tissue studied:	gastric cancer ( 3 ) , gastric carcinoma ( 3 )
Relevant cell line - cell type - tissue:	HeLa (cervical) ( 2 ) , Jurkat (T lymphocyte) ( 4 ) , MKN-45 (gastric) ( 3 )

Upstream Regulation
Kinases, in vitro:	Chk1 (human) ( 2 )

References
1	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
2	Blasius M, et al. (2011) A phospho-proteomic screen identifies substrates of the checkpoint kinase Chk1. Genome Biol 12, R78 21851590 Curated Info
3	Guo A (2011) CST Curation Set: 11302; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS/T) Immunoaffinity Beads Cat#: 1979 Curated Info
4	Possemato A (2010) CST Curation Set: 10792; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Curated Info