Ser13
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Home > Phosphorylation Site Page: > Ser13  -  CDC37 (human)

Site Information
VWDHIEVsDDEDETH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450034

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 6 ) , mass spectrometry ( 1 , 2 , 7 , 9 , 10 , 13 , 14 , 15 , 16 , 17 , 18 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ) , mutation of modification site ( 1 , 6 ) , phospho-antibody ( 1 , 5 , 7 , 11 , 12 , 28 ) , western blotting ( 1 , 6 , 7 , 11 , 12 , 28 )
Disease tissue studied:
brain cancer ( 7 ) , glioblastoma ( 7 ) , glioma ( 7 ) , breast cancer ( 5 , 6 , 9 , 14 , 17 ) , breast ductal carcinoma ( 14 ) , breast cancer, triple negative ( 14 ) , cervical cancer ( 24 , 28 ) , cervical adenocarcinoma ( 24 ) , cervical squamous cell carcinoma ( 28 ) , colorectal cancer ( 5 ) , colorectal carcinoma ( 5 ) , kidney cancer ( 1 ) , leukemia ( 20 ) , acute myelogenous leukemia ( 20 ) , lung cancer ( 17 ) , non-small cell lung cancer ( 17 ) , lymphoma ( 11 ) , B cell lymphoma ( 11 ) , non-Hodgkin's lymphoma ( 11 ) , follicular lymphoma ( 11 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 21 ) , 293 (epithelial) ( 1 , 25 ) , 786-O (renal) [VHL (human), transfection] ( 10 ) , 786-O (renal) ( 1 , 10 ) , A498 (renal) ( 1 , 23 ) , A549 (pulmonary) ( 15 ) , breast ( 14 ) , BT-20 (breast cell) ( 17 ) , BT-474 (breast cell) ( 5 , 9 ) , BT-549 (breast cell) ( 17 ) , Caki-1 (renal) ( 1 ) , Calu 6 (pulmonary) ( 17 ) , COS (fibroblast) ( 28 ) , E.coli (bacterial) ( 1 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 18 ) , Flp-In T-Rex-293 (epithelial) ( 18 ) , glial-brain ( 7 ) , H2009 (pulmonary) ( 17 ) , H2077 (pulmonary) ( 17 ) , H2887 (pulmonary) ( 17 ) , H322M (pulmonary) ( 17 ) , HCC1359 (pulmonary) ( 17 ) , HCC1937 (breast cell) ( 17 ) , HCC2279 (pulmonary) ( 17 ) , HCC366 (pulmonary) ( 17 ) , HCC4006 (pulmonary) ( 17 ) , HCC78 (pulmonary) ( 17 ) , HCT116 (intestinal) ( 5 ) , HeLa (cervical) ( 2 , 13 ) , HeLa S3 (cervical) ( 24 ) , HOP62 (pulmonary) ( 17 ) , HUES-9 ('stem, embryonic') ( 22 ) , Jurkat (T lymphocyte) ( 16 ) , KB (squamous) ( 28 ) , KG-1 (myeloid) ( 20 ) , LOU-NH91 (squamous) ( 17 ) , MCF-7 (breast cell) ( 6 , 9 , 17 ) , MDA-MB-231 (breast cell) ( 17 ) , MDA-MB-468 (breast cell) ( 17 ) , NCI-H1395 (pulmonary) ( 17 ) , NCI-H1648 (pulmonary) ( 17 ) , NCI-H2030 (pulmonary) ( 17 ) , NCI-H2172 (pulmonary) ( 17 ) , NCI-H322 (pulmonary) ( 17 ) , NCI-H460 (pulmonary) ( 17 ) , OCI-ly1 (B lymphocyte) ( 11 ) , OCI-ly10 (B lymphocyte) ( 11 ) , OCI-ly3 (B lymphocyte) ( 11 ) , SUM149 (breast cell) ( 5 ) , WM115 (melanocyte) ( 26 )

Upstream Regulation
Putative in vivo kinases:
CK2A2 (human) ( 28 )
Kinases, in vitro:
CK2A1 (human) ( 29 )
Phosphatases, in vitro:
PPP5C (human) ( 1 )
Treatments:
anisomycin ( 28 ) , IGF-1 ( 28 ) , OSU-03012 ( 7 ) , serum ( 28 ) , sildenafil ( 7 ) , silmitasertib ( 11 ) , tanespimycin ( 8 ) , TBB ( 28 ) , UV ( 28 )

Downstream Regulation
Effects of modification on CDC37:
molecular association, regulation ( 6 , 29 )
Induce interaction with:
HRI (human) ( 29 ) , HSP90A (human) ( 29 )

Disease / Diagnostics Relevance
Relevant diseases:
B cell lymphoma ( 11 ) , lymphoplasmacytic lymphoma ( 11 ) , non-Hodgkin's lymphoma ( 11 ) , follicular lymphoma ( 11 )

References 

1

Dushukyan N, et al. (2017) Phosphorylation and Ubiquitination Regulate Protein Phosphatase 5 Activity and Its Prosurvival Role in Kidney Cancer. Cell Rep 21, 1883-1895
29141220   Curated Info

2

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

5

Booth L, et al. (2016) [Pemetrexed + Sorafenib] lethality is increased by inhibition of ERBB1/2/3-PI3K-NFκB compensatory survival signaling. Oncotarget 7, 23608-32
27015562   Curated Info

6

Keramisanou D, et al. (2016) Molecular Mechanism of Protein Kinase Recognition and Sorting by the Hsp90 Kinome-Specific Cochaperone Cdc37. Mol Cell 62, 260-71
27105117   Curated Info

7

Booth L, et al. (2016) Multi-kinase inhibitors can associate with heat shock proteins through their NH2-termini by which they suppress chaperone function. Oncotarget 7, 12975-96
26887051   Curated Info

8

Jin J, et al. (2016) Mutational Analysis of Glycogen Synthase Kinase 3β Protein Kinase Together with Kinome-Wide Binding and Stability Studies Suggests Context-Dependent Recognition of Kinases by the Chaperone Heat Shock Protein 90. Mol Cell Biol 36, 1007-18
26755559   Curated Info

9

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

10

Malec V, Coulson JM, Urbé S, Clague MJ (2015) Combined Analyses of the VHL and Hypoxia Signaling Axes in an Isogenic Pairing of Renal Clear Cell Carcinoma Cells. J Proteome Res 14, 5263-72
26506913   Curated Info

11

Pizzi M, et al. (2015) Protein kinase CK2 is widely expressed in follicular, Burkitt and diffuse large B-cell lymphomas and propels malignant B-cell growth. Oncotarget 6, 6544-52
25788269   Curated Info

12

Smith JR, et al. (2015) Restricting direct interaction of CDC37 with HSP90 does not compromise chaperoning of client proteins. Oncogene 34, 15-26
24292678   Curated Info

13

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

14

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

15

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

16

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

17

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

18

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

19

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

20

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

21

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

22

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

23

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

24

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

25

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

26

Old WM, et al. (2009) Functional proteomics identifies targets of phosphorylation by B-Raf signaling in melanoma. Mol Cell 34, 115-31
19362540   Curated Info

27

Vaughan CK, et al. (2008) Hsp90-dependent activation of protein kinases is regulated by chaperone-targeted dephosphorylation of Cdc37. Mol Cell 31, 886-95
18922470   Curated Info

28

Miyata Y, Nishida E (2008) Evaluating CK2 activity with the antibody specific for the CK2-phosphorylated form of a kinase-targeting cochaperone Cdc37. Mol Cell Biochem 316, 127-34
18566753   Curated Info

29

Shao J, Prince T, Hartson SD, Matts RL (2003) Phosphorylation of serine 13 is required for the proper function of the Hsp90 co-chaperone, Cdc37. J Biol Chem 278, 38117-20
12930845   Curated Info