Ser1232
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser1232  -  NMDAR2A (rat)

Site Information
sGHFtMRsPFkCDAC   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448260

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , mass spectrometry ( 2 , 4 , 5 , 6 , 7 , 8 ) , mutation of modification site ( 9 , 10 ) , phospho-antibody ( 3 , 9 ) , phosphopeptide mapping ( 2 ) , western blotting ( 3 , 10 )
Relevant cell line - cell type - tissue:
'neuron, hippocampal'-brain ( 10 ) , 'neuron, hippocampal, CA1 pyramidal'-brain [CDK5 (rat)] ( 9 ) , 'neuron, hippocampal, CA1 pyramidal'-brain [NMDAR2A (rat)] ( 9 ) , 293T (epithelial) ( 10 ) , brain ( 4 , 5 , 6 , 7 , 8 ) , neuron ( 3 ) , neuron:synaptosome-brain ( 2 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (rat) ( 9 , 10 )
Kinases, in vitro:
CDK5 (rat) ( 10 )
Treatments:
glutamic acid ( 3 ) , ischemia ( 9 ) , ischemia/reperfusion ( 2 ) , painful stimulus ( 1 ) , seliciclib ( 1 , 10 )

Downstream Regulation
Effects of modification on NMDAR2A:
activity, induced ( 10 ) , intracellular localization ( 3 )

References 

1

Liu X, et al. (2014) Intrathecal administration of roscovitine prevents remifentanil-induced postoperative hyperalgesia and decreases the phosphorylation of N-methyl-D-aspartate receptor and metabotropic glutamate receptor 5 in spinal cord. Brain Res Bull 106, 9-16
24769228   Curated Info

2

Zhang F, et al. (2013) Phosphorylation and assembly of glutamate receptors after brain ischemia. Stroke 44, 170-6
23212166   Curated Info

3

Miao Y, et al. (2012) Involvement of Calpain/p35-p25/Cdk5/NMDAR Signaling Pathway in Glutamate-Induced Neurotoxicity in Cultured Rat Retinal Neurons. PLoS One 7, e42318
22870316   Curated Info

4

Guo A (2009) CST Curation Set: 5869; Year: 2009; Biosample/Treatment: tissue, brain/No seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Guo A (2008) CST Curation Set: 5217; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Guo A (2008) CST Curation Set: 5219; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Guo A (2008) CST Curation Set: 5221; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Guo A (2008) CST Curation Set: 5222; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Wang J, et al. (2003) Cdk5 activation induces hippocampal CA1 cell death by directly phosphorylating NMDA receptors. Nat Neurosci 6, 1039-47
14502288   Curated Info

10

Li BS, et al. (2001) Regulation of NMDA receptors by cyclin-dependent kinase-5. Proc Natl Acad Sci U S A 98, 12742-7
11675505   Curated Info