Ser617
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Home > Phosphorylation Site Page: > Ser617  -  eNOS (cow)

Site Information
SYKIRFNsVSCSDPL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448656

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 8 ) , mutation of modification site ( 4 , 7 , 8 , 9 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , phosphopeptide mapping ( 8 ) , western blotting ( 1 , 3 , 4 , 5 , 8 , 9 )
Relevant cell line - cell type - tissue:
BAEC (endothelial) ( 4 , 7 ) , BAE ( 3 , 8 ) , COS (fibroblast) ( 4 , 7 , 9 ) , endothelial-aorta ( 5 , 6 ) , PPAEC (endothelial) ( 1 )

Upstream Regulation
Regulatory protein:
eNOS (cow) ( 4 ) , PTEN (human) ( 9 )
Kinases, in vitro:
Akt1 (human) ( 2 )
Treatments:
17-beta-estradiol ( 1 ) , apoA-I ( 6 ) , ATP ( 7 , 8 ) , BAPTA-AM ( 8 ) , bradykinin ( 8 ) , calyculin_A ( 4 ) , HDL ( 6 ) , IBMX ( 8 ) , ionomycin ( 4 ) , KT5720 ( 8 ) , LDL ( 6 ) , lovastatin ( 5 ) , LY294002 ( 5 , 8 ) , pravastatin ( 5 ) , quercetin ( 3 ) , VEGF ( 7 , 8 ) , wortmannin ( 5 , 8 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, induced ( 2 , 4 ) , molecular association, regulation ( 7 ) , phosphorylation ( 7 )
Inhibit interaction with:
Akt1 (human) ( 7 ) , HSP90A (human) ( 7 )

References 

1

Tran QK, et al. (2016) Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1. J Biol Chem 291, 10805-23
26987903   Curated Info

2

Tran QK, et al. (2009) Effects of combined phosphorylation at Ser-617 and Ser-1179 in endothelial nitric-oxide synthase on EC50(Ca2+) values for calmodulin binding and enzyme activation. J Biol Chem 284, 11892-9
19251696   Curated Info

3

Jackson SJ, Venema RC (2006) Quercetin inhibits eNOS, microtubule polymerization, and mitotic progression in bovine aortic endothelial cells. J Nutr 136, 1178-84
16614401   Curated Info

4

Church JE, Fulton D (2006) Differences in eNOS activity because of subcellular localization are dictated by phosphorylation state rather than the local calcium environment. J Biol Chem 281, 1477-88
16257964   Curated Info

5

Harris MB, et al. (2004) Acute activation and phosphorylation of endothelial nitric oxide synthase by HMG-CoA reductase inhibitors. Am J Physiol Heart Circ Physiol 287, H560-6
15087285   Curated Info

6

Drew BG, et al. (2004) High-density lipoprotein and apolipoprotein AI increase endothelial NO synthase activity by protein association and multisite phosphorylation. Proc Natl Acad Sci U S A 101, 6999-7004
15107497   Curated Info

7

Bauer PM, et al. (2003) Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase. J Biol Chem 278, 14841-9
12591925   Curated Info

8

Michell BJ, et al. (2002) Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635. J Biol Chem 277, 42344-51
12171920   Curated Info

9

Church JE, et al. Inhibition of endothelial nitric oxide synthase by the lipid phosphatase PTEN. Vascul Pharmacol 52, 191-8
19962452   Curated Info