Thr11

Site Information
QRQRsLstsGEsLyH SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 486100

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 1 ) , 'brain, cerebral cortex' ( 8 , 11 ) , 3T3-L1 (fibroblast) ( 9 ) , brain ( 6 ) , heart ( 6 ) , kidney ( 6 ) , liver ( 3 ) , liver [leptin (mouse), homozygous knockout] ( 3 ) , lung ( 6 ) , macrophage-peritoneum ( 2 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 5 ) , mpkCCD (renal) ( 7 ) , P19 (testicular) ( 10 ) , spleen ( 6 ) , testis ( 6 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 )

Relevant cell line - cell type - tissue:

'3T3-L1, differentiated' (adipocyte) ( 1 ) , 'brain, cerebral cortex' ( 8 , 11 ) , 3T3-L1 (fibroblast) ( 9 ) , brain ( 6 ) , heart ( 6 ) , kidney ( 6 ) , liver ( 3 ) , liver [leptin (mouse), homozygous knockout] ( 3 ) , lung ( 6 ) , macrophage-peritoneum ( 2 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 5 ) , mpkCCD (renal) ( 7 ) , P19 (testicular) ( 10 ) , spleen ( 6 ) , testis ( 6 )

Upstream Regulation
Treatments:	retinoic_acid ( 10 )

References
1	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
2	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
3	Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83 23140645 Curated Info
4	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
5	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
6	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
7	Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7 20139300 Curated Info
8	Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82 19737024 Curated Info
9	Guo A (2009) CST Curation Set: 7448; Year: 2009; Biosample/Treatment: cell line, 3T3-L1/control; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info
10	Smith JC, et al. (2007) A differential phosphoproteomic analysis of retinoic acid-treated P19 cells. J Proteome Res 6, 3174-86 17622165 Curated Info
11	Munton RP, et al. (2007) Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations. Mol Cell Proteomics 6, 283-93 17114649 Curated Info