Thr130
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Home > Phosphorylation Site Page: > Thr130  -  CDC25C (human)

Site Information
PAQLLCStPNGLDRG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449911

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 10 ) , [32P] bio-synthetic labeling ( 10 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 ) , mutation of modification site ( 5 , 9 ) , peptide sequencing ( 10 ) , phospho-antibody ( 5 , 9 ) , phosphoamino acid analysis ( 10 ) , phosphopeptide mapping ( 10 ) , western blotting ( 5 , 9 )
Disease tissue studied:
bone cancer ( 5 ) , colorectal cancer ( 9 ) , colorectal carcinoma ( 9 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 , 9 ) , fibroblast ( 5 ) , HCT116 (intestinal) ( 9 ) , HeLa (cervical) ( 1 , 2 , 5 , 6 , 7 , 8 , 9 ) , Jurkat (T lymphocyte) ( 4 ) , oocyte ( 9 ) , REF52 (fibroblast) ( 10 ) , U2OS (bone cell) ( 5 )

Upstream Regulation
Kinases, in vitro:
CDK1 (human) ( 10 )
Treatments:
nocodazole ( 9 )

Downstream Regulation
Effects of modification on biological processes:
cell adhesion, altered ( 10 ) , cell cycle regulation ( 5 , 10 )

References 

1

Zhou J (2010) CST Curation Set: 10691; Year: 2010; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb Cat#: 5243, PTMScan(R) Phospho-PLK Binding Motif (ST*P) Immunoaffinity Beads Cat#: 5756
Curated Info

2

Zhou J (2010) CST Curation Set: 10690; Year: 2010; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb Cat#: 5243, PTMScan(R) Phospho-PLK Binding Motif (ST*P) Immunoaffinity Beads Cat#: 5756
Curated Info

3

Guo A (2010) CST Curation Set: 9969; Year: 2010; Biosample/Treatment: cell line, 293/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Millipore 05-368
Curated Info

4

Possemato A (2010) CST Curation Set: 9437; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb Cat#: 5243, PTMScan(R) Phospho-PLK Binding Motif (ST*P) Immunoaffinity Beads Cat#: 5756
Curated Info

5

Franckhauser C, et al. (2010) Distinct pools of cdc25C are phosphorylated on specific TP sites and differentially localized in human mitotic cells. PLoS One 5, e11798
20668692   Curated Info

6

Moritz A (2008) CST Curation Set: 5060; Year: 2008; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

7

Moritz A (2008) CST Curation Set: 5061; Year: 2008; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

8

Moritz A (2008) CST Curation Set: 5071; Year: 2008; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

9

Margolis SS, et al. (2006) Role for the PP2A/B56delta phosphatase in regulating 14-3-3 release from Cdc25 to control mitosis. Cell 127, 759-73
17110335   Curated Info

10

Strausfeld U, et al. (1994) Activation of p34cdc2 protein kinase by microinjection of human cdc25C into mammalian cells. Requirement for prior phosphorylation of cdc25C by p34cdc2 on sites phosphorylated at mitosis. J Biol Chem 269, 5989-6000
8119945   Curated Info

11

Izumi T, Maller JL (1993) Elimination of cdc2 phosphorylation sites in the cdc25 phosphatase blocks initiation of M-phase. Mol Biol Cell 4, 1337-50
7513216   Curated Info

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