Tyr1015
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Home > Phosphorylation Site Page: > Tyr1015  -  Ret (human)

Site Information
MMVKRRDyLDLAAST   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449894

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , microscopy-colocalization with upstream kinase ( 7 ) , mutation of modification site ( 2 , 3 , 5 , 6 , 7 , 8 , 10 ) , phospho-antibody ( 2 , 4 , 5 , 7 , 8 , 9 ) , phosphopeptide mapping ( 10 ) , western blotting ( 2 , 4 , 7 , 8 )
Disease tissue studied:
adrenal cancer ( 7 ) , pheochromocytoma ( 7 ) , neuroblastoma ( 8 ) , thyroid cancer ( 4 )
Relevant cell line - cell type - tissue:
'neuron, superior cervical ganglion'-brain [Ret (mouse), homozygous knockout] ( 3 ) , 293 (epithelial) ( 2 , 5 ) , COS (fibroblast) ( 7 , 8 , 10 ) , HEK293T (epithelial) ( 4 ) , MG87 (fibroblast) ( 8 ) , MN1 (neuron) ( 8 ) , Neuro-2a (neuron) ( 8 ) , neuron-dorsal root ganglia ( 8 ) , PC CL 3 (thyroid cell) ( 6 ) , PC-12 (chromaffin) ( 7 ) , thyroid ( 7 ) , TPC-1 (thyroid cell) ( 4 , 9 ) , TT (thyroid cell) ( 9 )

Upstream Regulation
Regulatory protein:
Ret (human) ( 7 )
Kinases, in vitro:
Ret (human) ( 8 , 10 )
Putative upstream phosphatases:
PTPRJ (human) ( 4 )
Treatments:
ART ( 8 ) , GDNF ( 5 , 8 ) , neurturin ( 8 )

Downstream Regulation
Effects of modification on biological processes:
cell growth, altered ( 7 ) , transcription, altered ( 6 )

References 

1

Plaza-Menacho I, et al. (2016) RET Functions as a Dual-Specificity Kinase that Requires Allosteric Inputs from Juxtamembrane Elements. Cell Rep 17, 3319-3332
28009299   Curated Info

2

Lodyga M, et al. (2009) XB130, a tissue-specific adaptor protein that couples the RET/PTC oncogenic kinase to PI 3-kinase pathway. Oncogene 28, 937-49
19060924   Curated Info

3

Encinas M, et al. (2008) Analysis of Ret knockin mice reveals a critical role for IKKs, but not PI 3-K, in neurotrophic factor-induced survival of sympathetic neurons. Cell Death Differ 15, 1510-21
18497757   Curated Info

4

Iervolino A, et al. (2006) The receptor-type protein tyrosine phosphatase J antagonizes the biochemical and biological effects of RET-derived oncoproteins. Cancer Res 66, 6280-7
16778204   Curated Info

5

Encinas M, Crowder RJ, Milbrandt J, Johnson EM (2004) Tyrosine 981, a novel ret autophosphorylation site, binds c-Src to mediate neuronal survival. J Biol Chem 279, 18262-9
14766744   Curated Info

6

Castellone MD, et al. (2004) Autocrine stimulation by osteopontin plays a pivotal role in the expression of the mitogenic and invasive phenotype of RET/PTC-transformed thyroid cells. Oncogene 23, 2188-96
14981541   Curated Info

7

Buckwalter TL, et al. (2002) The roles of phosphotyrosines-294, -404, and -451 in RET/PTC1-induced thyroid tumor formation. Oncogene 21, 8166-72
12444552   Curated Info

8

Coulpier M, Anders J, Ibáñez CF (2002) Coordinated activation of autophosphorylation sites in the RET receptor tyrosine kinase: importance of tyrosine 1062 for GDNF mediated neuronal differentiation and survival. J Biol Chem 277, 1991-9
11713247   Curated Info

9

Salvatore D, et al. (2000) Tyrosines 1015 and 1062 are in vivo autophosphorylation sites in ret and ret-derived oncoproteins. J Clin Endocrinol Metab 85, 3898-907
11061555   Curated Info

10

Liu X, et al. (1996) Oncogenic RET receptors display different autophosphorylation sites and substrate binding specificities. J Biol Chem 271, 5309-12
8621380   Curated Info