Tyr1096
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Home > Phosphorylation Site Page: > Tyr1096  -  Ret (human)

Site Information
RyPNDSVyANWMLSP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449891
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 11 ) , mass spectrometry ( 1 , 2 , 4 , 5 , 6 ) , mutation of modification site ( 7 , 9 , 10 , 11 ) , phospho-antibody ( 3 , 9 ) , phosphopeptide mapping ( 11 ) , western blotting ( 3 , 9 )
Disease tissue studied:
bone cancer ( 2 ) , Ewing's sarcoma ( 2 ) , breast cancer ( 6 ) , neuroblastoma ( 1 , 4 , 5 , 9 )
Relevant cell line - cell type - tissue:
'neuron, superior cervical ganglion'-brain ( 10 ) , 3T3 (fibroblast) ( 10 ) , A673 (muscle cell) ( 2 ) , COS (fibroblast) ( 7 , 9 , 11 ) , EVSA-T (breast cell) ( 6 ) , LAN-6 (neural crest) ( 1 ) , MG87 (fibroblast) ( 9 , 10 ) , MN1 (neuron) ( 9 ) , Neuro-2a (neuron) ( 9 ) , neuron-dorsal root ganglia ( 9 ) , SH-SY5Y (neural crest) ( 1 ) , SiMa (neural crest) ( 4 ) , SKNFI (neural crest) ( 1 , 5 )

Upstream Regulation
Kinases, in vitro:
Ret (human) ( 8 , 9 , 11 )
Treatments:
ART ( 9 ) , GDNF ( 3 , 9 , 10 ) , neurturin ( 9 )

Downstream Regulation
Effects of modification on Ret:
molecular association, regulation ( 7 , 10 )
Induce interaction with:
GRB2 (human) ( 7 , 10 ) , PIK3R1 (human) ( 10 )

References 

1

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

2

Bai Y, et al. (2012) Phosphoproteomics identifies driver tyrosine kinases in sarcoma cell lines and tumors. Cancer Res 72, 2501-11
22461510   Curated Info

3

Runeberg-Roos P, Virtanen H, Saarma M (2007) RET(MEN 2B) is active in the endoplasmic reticulum before reaching the cell surface. Oncogene 26, 7909-15
17599050   Curated Info

4

Stokes M (2006) CST Curation Set: 1888; Year: 2006; Biosample/Treatment: cell line, SiMa/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Stokes M (2006) CST Curation Set: 1764; Year: 2006; Biosample/Treatment: cell line, SKNFI/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Michaud C (2006) CST Curation Set: 1701; Year: 2006; Biosample/Treatment: cell line, EVSA-T/serum starved; Disease: breast cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Scott RP, Eketjäll S, Aineskog H, Ibáñez CF (2005) Distinct turnover of alternatively spliced isoforms of the RET kinase receptor mediated by differential recruitment of the Cbl ubiquitin ligase. J Biol Chem 280, 13442-9
15677445   Curated Info

8

Kawamoto Y, et al. (2004) Identification of RET autophosphorylation sites by mass spectrometry. J Biol Chem 279, 14213-24
14711813   Curated Info

9

Coulpier M, Anders J, Ibáñez CF (2002) Coordinated activation of autophosphorylation sites in the RET receptor tyrosine kinase: importance of tyrosine 1062 for GDNF mediated neuronal differentiation and survival. J Biol Chem 277, 1991-9
11713247   Curated Info

10

Besset V, Scott RP, Ibáñez CF (2000) Signaling complexes and protein-protein interactions involved in the activation of the Ras and phosphatidylinositol 3-kinase pathways by the c-Ret receptor tyrosine kinase. J Biol Chem 275, 39159-66
10995764   Curated Info

11

Liu X, et al. (1996) Oncogenic RET receptors display different autophosphorylation sites and substrate binding specificities. J Biol Chem 271, 5309-12
8621380   Curated Info