Ser16
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser16  -  PSMA5 (human)

Site Information
DrGVNtFsPEGrLFQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 483589

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 12 , 13 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
breast cancer ( 1 , 7 , 13 ) , breast ductal carcinoma ( 7 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 7 ) , cervical cancer ( 22 ) , cervical adenocarcinoma ( 22 ) , leukemia ( 15 ) , acute myelogenous leukemia ( 15 ) , lung cancer ( 1 , 9 , 13 ) , non-small cell lung cancer ( 1 , 13 ) , non-small cell lung adenocarcinoma ( 1 , 9 ) , neuroblastoma ( 1 ) , ovarian cancer ( 7 ) , pancreatic ductal adenocarcinoma ( 8 ) , melanoma skin cancer ( 5 )
Relevant cell line - cell type - tissue:
'pancreatic, ductal'-pancreas ( 8 ) , 293 (epithelial) ( 24 ) , breast ( 2 , 7 ) , BT-20 (breast cell) ( 13 ) , BT-549 (breast cell) ( 13 ) , Calu 6 (pulmonary) ( 13 ) , H2077 (pulmonary) ( 13 ) , H322M (pulmonary) ( 13 ) , HCC1937 (breast cell) ( 13 ) , HCC2279 (pulmonary) ( 13 ) , HCC366 (pulmonary) ( 13 ) , HCC78 (pulmonary) ( 13 ) , HCC827 (pulmonary) ( 13 ) , HCT116 (intestinal) ( 25 ) , HEK293-A (epithelial) ( 1 ) , HEK293T (epithelial) ( 1 , 4 ) , HeLa (cervical) ( 6 , 12 , 16 , 20 , 23 , 25 , 28 , 30 , 31 ) , HeLa S3 (cervical) [PLK1 (human), knockdown, Tet-inducible PLK1 siRNA] ( 18 ) , HeLa S3 (cervical) ( 18 , 22 , 27 ) , HeLa_Meta (cervical) ( 21 ) , HeLa_Pro (cervical) ( 21 ) , HeLa_Telo (cervical) ( 21 ) , HOP62 (pulmonary) ( 13 ) , HUES-9 ('stem, embryonic') ( 19 ) , Jurkat (T lymphocyte) ( 10 , 17 , 26 , 29 ) , K562 (erythroid) ( 12 , 23 ) , KG-1 (myeloid) ( 15 ) , LCLC-103H (pulmonary) ( 13 ) , LOU-NH91 (squamous) ( 13 ) , lung ( 9 ) , MCF-7 (breast cell) ( 1 , 13 ) , MDA-MB-231 (breast cell) ( 13 ) , MDA-MB-468 (breast cell) ( 13 ) , NCI-H1395 (pulmonary) ( 13 ) , NCI-H1568 (pulmonary) ( 13 ) , NCI-H157 (pulmonary) ( 13 ) , NCI-H1648 (pulmonary) ( 13 ) , NCI-H1666 (pulmonary) ( 13 ) , NCI-H1975 (pulmonary) ( 1 ) , NCI-H2030 (pulmonary) ( 13 ) , NCI-H2172 (pulmonary) ( 13 ) , NCI-H322 (pulmonary) ( 13 ) , NCI-H460 (pulmonary) ( 13 , 25 ) , NCI-H520 (squamous) ( 13 ) , NCI-H647 (pulmonary) ( 13 ) , Neuro-2a (neuron) ( 1 ) , ovary ( 7 ) , PC9 (pulmonary) ( 13 ) , WM239A (melanocyte) ( 5 )

Upstream Regulation
Putative in vivo kinases:
CDK1 (human) ( 1 )
Treatments:
BI_4834 ( 16 ) , cold_shock ( 1 ) , dinaciclib ( 1 ) , ischemia ( 7 ) , MG132_withdrawal ( 21 ) , nocodazole ( 1 , 22 , 25 ) , RO-3306 ( 1 )

Downstream Regulation
Effects of modification on PSMA5:
molecular association, regulation ( 1 ) , phosphorylation ( 1 )
Effects of modification on biological processes:
cell cycle regulation ( 1 ) , cell growth, induced ( 1 ) , signaling pathway regulation ( 1 )
Induce interaction with:
AMPD2 (human) ( 1 ) , CLCC1 (human) ( 1 ) , PKN2 (human) ( 1 ) , PLK1 (human) ( 1 ) , RP1 (human) ( 1 )

References 

1

Duan J, et al. (2021) Conserved Mitotic Phosphorylation of a Proteasome Subunit Regulates Cell Proliferation. Cells 10
34831298   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

4

Franchin C, et al. (2015) Quantitative analysis of a phosphoproteome readily altered by the protein kinase CK2 inhibitor quinalizarin in HEK-293T cells. Biochim Biophys Acta 1854, 609-23
25278378   Curated Info

5

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

6

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

7

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

8

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

9

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

10

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

11

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

12

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

13

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

14

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

15

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

16

Grosstessner-Hain K, et al. (2011) Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics 10, M111.008540
21857030   Curated Info

17

Guo A (2011) CST Curation Set: 11733; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

18

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

19

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

20

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

21

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

22

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

23

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

24

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

25

Nagano K, et al. (2009) Phosphoproteomic analysis of distinct tumor cell lines in response to nocodazole treatment. Proteomics 9, 2861-74
19415658   Curated Info

26

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

27

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

28

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

29

Stokes M (2008) CST Curation Set: 3885; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

30

Yu LR, et al. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra. J Proteome Res 6, 4150-62
17924679   Curated Info

31

Beausoleil SA, et al. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-92
16964243   Curated Info