Ser1043
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Home > Phosphorylation Site Page: > Ser1043  -  BUB1B (human)

Site Information
WkVGkLtsPGALLFQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 483373

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 10 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , mutation of modification site ( 1 , 7 , 10 ) , phospho-antibody ( 10 ) , western blotting ( 1 )
Disease tissue studied:
cervical cancer ( 7 , 8 ) , cervical adenocarcinoma ( 7 , 8 ) , leukemia ( 2 ) , acute myelogenous leukemia ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 9 ) , HeLa (cervical) ( 1 , 3 , 5 , 6 , 10 , 12 ) , HeLa S3 (cervical) ( 7 , 8 , 11 ) , HeLa_BI (cervical) ( 4 ) , HeLa_Hesp (cervical) ( 4 ) , HeLa_NOC (cervical) ( 4 ) , KG-1 (myeloid) ( 2 )

Upstream Regulation
Putative in vivo kinases:
TTK (human) ( 10 )
Kinases, in vitro:
CDK1 (human) ( 7 )
Treatments:
AZ3146 ( 1 ) , BI2536 ( 1 ) , hesperadin ( 10 ) , nocodazole ( 10 ) , seliciclib ( 10 ) , SP600125 ( 1 ) , staurosporine ( 10 ) , ZM447439 ( 1 )

Downstream Regulation
Effects of modification on biological processes:
cell cycle regulation ( 10 )

References 

1

Tipton AR, et al. (2013) Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex. J Biol Chem 288, 35149-58
24151075   Curated Info

2

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

3

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

4

Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12
22067460   Curated Info

5

Zhou J (2010) CST Curation Set: 10709; Year: 2010; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

6

Zhou J (2010) CST Curation Set: 10684; Year: 2010; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

7

Elowe S, et al. (2010) Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1. J Cell Sci 123, 84-94
20016069   Curated Info

8

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

9

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

10

Huang H, et al. (2008) Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit. J Cell Biol 183, 667-80
19015317   Curated Info

11

Steen JA, et al. (2008) Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A 105, 6069-74
18420821   Curated Info

12

Beausoleil SA, et al. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-92
16964243   Curated Info