Thr2511
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Home > Phosphorylation Site Page: > Thr2511  -  Notch 1 (human)

Site Information
VPEHPFLtPsPEsPD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 483083

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 5 ) , electrophoretic mobility shift ( 2 ) , immunoprecipitation ( 2 , 3 , 4 , 5 ) , mass spectrometry ( 5 ) , microscopy-colocalization with upstream kinase ( 2 ) , mutation of modification site ( 2 , 3 , 4 , 5 ) , phospho-antibody ( 4 , 5 ) , western blotting ( 2 , 3 , 4 , 5 )
Disease tissue studied:
breast cancer ( 2 , 4 ) , breast adenocarcinoma ( 2 ) , breast cancer, triple negative ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CDK19 (human) ( 5 ) , CDK3 (human) ( 5 ) , CDK8 (human) ( 5 ) , DYRK2 (human) ( 2 ) , HIPK2 (human) ( 4 ) , MEKK1 (human) ( 3 )
Kinases, in vitro:
CDK19 (human) ( 5 ) , CDK3 (human) ( 5 ) , CDK8 (human) ( 5 ) , DYRK2 (human) ( 2 )
Treatments:
1NM-PP1 ( 2 ) , adriamycin ( 2 , 4 ) , CPT ( 2 ) , curcumin ( 2 ) , etoposide ( 2 ) , harmine ( 2 ) , siRNA ( 2 )

Downstream Regulation
Effects of modification on Notch 1:
activity, inhibited ( 2 ) , molecular association, regulation ( 5 ) , protein degradation ( 2 , 3 , 4 , 5 ) , ubiquitination ( 2 , 3 , 4 , 5 )
Effects of modification on biological processes:
carcinogenesis, altered ( 2 ) , carcinogenesis, inhibited ( 5 ) , cell growth, inhibited ( 4 ) , cell motility, altered ( 2 ) , cell motility, inhibited ( 4 ) , signaling pathway regulation ( 2 ) , transcription, inhibited ( 2 )
Induce interaction with:
FBXW7 (human) ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
breast cancer ( 4 )

References 

1

Choi HY, et al. (2024) NOTCH localizes to mitochondria through the TBC1D15-FIS1 interaction and is stabilized via blockade of E3 ligase and CDK8 recruitment to reprogram tumor-initiating cells. Exp Mol Med 56, 461-477
38409448   Curated Info

2

Morrugares R, et al. (2019) Phosphorylation-dependent regulation of the NOTCH1 intracellular domain by dual-specificity tyrosine-regulated kinase 2. Cell Mol Life Sci
31605148   Curated Info

3

Ahn JS, et al. (2016) Autophagy negatively regulates tumor cell proliferation through phosphorylation dependent degradation of the Notch1 intracellular domain. Oncotarget 7, 79047-79063
27806347   Curated Info

4

Ann EJ, et al. (2016) Tumor Suppressor HIPK2 Regulates Malignant Growth via Phosphorylation of Notch1. Cancer Res 76, 4728-40
27335110   Curated Info

5

Li N, et al. (2014) Cyclin C is a haploinsufficient tumour suppressor. Nat Cell Biol 16, 1080-91
25344755   Curated Info