Ser59
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Home > Phosphorylation Site Page: > Ser59  -  CRYAB (mouse)

Site Information
PSFLRAPsWIDTGLS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448783

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 , 4 , 5 , 7 , 9 ) , mutation of modification site ( 1 , 11 ) , phospho-antibody ( 1 , 6 , 8 , 10 , 11 , 12 , 13 , 14 ) , western blotting ( 1 , 6 , 8 , 10 , 11 , 14 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
ADRB1 (mouse) ( 5 ) , MKK6 (human) ( 14 ) , PKN1 (mouse) ( 6 )
Putative in vivo kinases:
P38A (human) ( 10 , 12 ) , PKN1 (mouse) ( 6 )
Treatments:
arsenite ( 14 ) , bisindolylmaleimide ( 12 ) , cytochalasin_D ( 12 ) , H-89 ( 12 ) , heat_shock ( 12 ) , hyperoxia ( 8 ) , ischemia/reperfusion ( 10 , 11 ) , PD98059 ( 14 ) , PDGF ( 13 ) , SB202190 ( 11 ) , SB203580 ( 12 , 13 , 14 ) , sorbitol ( 14 ) , vinblastine ( 12 ) , virus infection ( 1 ) , Y27632 ( 12 )

Downstream Regulation
Effects of modification on CRYAB:
intracellular localization ( 10 ) , protein degradation ( 1 )
Effects of modification on biological processes:
apoptosis, inhibited ( 14 ) , cytoskeletal reorganization ( 12 )

References 

1

Fung G, et al. (2017) Phosphorylation and degradation of αB-crystallin during enterovirus infection facilitates viral replication and induces viral pathogenesis. Oncotarget 8, 74767-74780
29088822   Curated Info

2

Kuipers HF, et al. (2017) Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A 114, E1745-E1754
28196893   Curated Info

3

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

6

Takagi H, et al. (2010) Activation of PKN mediates survival of cardiac myocytes in the heart during ischemia/reperfusion. Circ Res 107, 642-9
20595653   Curated Info

7

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

8

Kase S, et al. (2010) alphaB-crystallin regulation of angiogenesis by modulation of VEGF. Blood 115, 3398-406
20023214   Curated Info

9

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

10

Whittaker R, et al. (2009) Kinetics of the translocation and phosphorylation of alphaB-crystallin in mouse heart mitochondria during ex vivo ischemia. Am J Physiol Heart Circ Physiol 296, H1633-42
19252088   Curated Info

11

Jin JK, et al. (2008) Localization of phosphorylated {alpha}B-crystallin to heart mitochondria during ischemia-reperfusion. Am J Physiol Heart Circ Physiol 294, H337-44
17993600   Curated Info

12

Launay N, et al. (2006) Cell signaling pathways to alphaB-crystallin following stresses of the cytoskeleton. Exp Cell Res 312, 3570-84
16979163   Curated Info

13

Shu E, et al. (2005) alphaB-crystallin is phosphorylated during myocardial infarction: involvement of platelet-derived growth factor-BB. Arch Biochem Biophys 438, 111-8
15907784   Curated Info

14

Hoover HE, Thuerauf DJ, Martindale JJ, Glembotski CC (2000) alpha B-crystallin gene induction and phosphorylation by MKK6-activated p38. A potential role for alpha B-crystallin as a target of the p38 branch of the cardiac stress response. J Biol Chem 275, 23825-33
10816593   Curated Info