Thr349
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Home > Phosphorylation Site Page: > Thr349  -  MLKL (mouse)

Site Information
TQNsIsRtAKSTKAE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 8148779

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 3 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , fibrosarcoma of soft tissue ( 1 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 1 ) , C2C12 (myoblast) ( 3 ) , fibroblast-embryo ( 1 ) , HeLa (cervical) ( 1 ) , HT-29 (intestinal) ( 1 ) , L929 (fibroblast) ( 1 ) , SVEC (endothelial) ( 1 )

Upstream Regulation
Putative in vivo kinases:
RIPK3 (human) ( 1 )
Kinases, in vitro:
RIPK3 (mouse) ( 2 )

Downstream Regulation
Effects of modification on MLKL:
protein conformation ( 1 )

References 

1

Rodriguez DA, et al. (2016) Characterization of RIPK3-mediated phosphorylation of the activation loop of MLKL during necroptosis. Cell Death Differ 23, 76-88
26024392   Curated Info

2

Murphy JM, et al. (2013) The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism. Immunity 39, 443-53
24012422   Curated Info

3

Possemato A (2008) CST Curation Set: 5233; Year: 2008; Biosample/Treatment: cell line, C2C12/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info