Thr300
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Home > Phosphorylation Site Page: > Thr300  -  NUSAP1 (human)

Site Information
HKRsLtKtPARksAH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 7989914

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 1 ) , mass spectrometry ( 2 , 3 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CDK1 (human) ( 1 )
Kinases, in vitro:
CDK1 (human) ( 1 )
Treatments:
nocodazole ( 1 ) , seliciclib ( 1 )

Downstream Regulation
Effects of modification on NUSAP1:
intracellular localization ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 1 )

References 

1

Chou HY, et al. (2011) Phosphorylation of NuSAP by Cdk1 regulates its interaction with microtubules in mitosis. Cell Cycle 10, 4083-9
22101338   Curated Info

2

Guo A (2011) CST Curation Set: 12023; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

3

Zhou J (2009) CST Curation Set: 6239; Year: 2009; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R)Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info