Ser44
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Home > Phosphorylation Site Page: > Ser44  -  ROMK (rat)

Site Information
RQRARLVsKEGRCNI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449605

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 2 , 3 , 4 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
NRK-52E (epithelial) ( 1 ) , oocyte ( 2 , 4 )

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 2 ) , SGK1 (rat) ( 4 )
Treatments:
colforsin ( 5 ) , gabapentin ( 2 ) , H-89 ( 2 ) , HGF ( 1 ) , IBMX ( 5 ) , KT5720 ( 2 )

Downstream Regulation
Effects of modification on ROMK:
activity, induced ( 2 , 4 ) , intracellular localization ( 1 , 3 ) , protein conformation ( 2 )

References 

1

Kato T, Oka K, Nakamura T (2018) HGF induces the serine‑phosphorylation and cell surface translocation of ROMK (Kir 1.1) channels in rat kidney cells. Mol Med Rep 17, 1031-1034
29115510   Curated Info

2

Lee CH, Tsai TS, Liou HH (2008) Gabapentin activates ROMK1 channels by a protein kinase A (PKA)-dependent mechanism. Br J Pharmacol 154, 216-25
18311184   Curated Info

3

Yoo D, et al. (2005) A phosphorylation-dependent export structure in ROMK (Kir 1.1) channel overrides an endoplasmic reticulum localization signal. J Biol Chem 280, 35281-9
16118216   Curated Info

4

Palmada M, et al. (2003) Negative charge at the consensus sequence for the serum- and glucocorticoid-inducible kinase, SGK1, determines pH sensitivity of the renal outer medullary K+ channel, ROMK1. Biochem Biophys Res Commun 307, 967-72
12878206   Curated Info

5

Xu ZC, Yang Y, Hebert SC (1996) Phosphorylation of the ATP-sensitive, inwardly rectifying K+ channel, ROMK, by cyclic AMP-dependent protein kinase. J Biol Chem 271, 9313-9
8621594   Curated Info

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