Ser277

Site Information
RRRssCVsLGEsAAG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 479408

In vivo Characterization
Methods used to characterize site in vivo:	2D analysis ( 5 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , phosphopeptide mapping ( 5 )
Disease tissue studied:	liver cancer ( 5 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 1 ) , 3T3-L1 (fibroblast) ( 3 , 4 ) , adipocyte ( 5 ) , H4IIe (hepatic) ( 5 ) , liver ( 2 ) , liver [leptin (mouse), homozygous knockout] ( 2 )

Upstream Regulation
Treatments:	calyculin_A ( 5 ) , insulin ( 1 , 5 ) , LY294002 ( 1 ) , MK-2206 ( 1 )

References
1	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
2	Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83 23140645 Curated Info
3	Guo A (2009) CST Curation Set: 7449; Year: 2009; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info
4	Guo A (2009) CST Curation Set: 7451; Year: 2009; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS/T) Immunoaffinity Beads Cat#: 1979 Curated Info
5	Lindh R, et al. (2007) Multisite phosphorylation of adipocyte and hepatocyte phosphodiesterase 3B. Biochim Biophys Acta 1773, 584-92 17320989 Curated Info