Thr354
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr354  -  SSTR2 (rat)

Site Information
KsRLNEttEtQRtLL   SwissProt
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 7652101

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 4 , 5 ) , phospho-antibody ( 2 , 3 , 4 , 5 ) , western blotting ( 2 , 3 , 4 , 5 )
Disease tissue studied:
pancreatic cancer ( 4 ) , pancreatic carcinoma ( 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 , 4 , 5 ) , CHO (fibroblast) ( 2 , 5 ) , GH3 (epithelial) ( 4 ) , GH4C1 (pituitary cell) [SSTR2 (rat), transfection] ( 5 ) , INS-1 (pancreatic) ( 4 ) , pancreas ( 4 ) , pituitary gland ( 4 )

Upstream Regulation
Putative in vivo kinases:
GRK2 (human) ( 4 ) , GRK3 (human) ( 4 )
Treatments:
BIM23627 ( 4 ) , KE108 ( 2 ) , L779,976 ( 4 ) , octreotide ( 3 , 4 ) , pasireotide ( 2 , 3 , 4 ) , phorbol_ester ( 4 ) , siRNA ( 4 ) , SST14 ( 2 , 3 , 4 , 5 )

Downstream Regulation
Effects of modification on SSTR2:
receptor internalization, altered ( 2 )

References 

1

Pöll F, Doll C, Schulz S (2011) Rapid Dephosphorylation of G Protein-coupled Receptors by Protein Phosphatase 1{beta} Is Required for Termination of {beta}-Arrestin-dependent Signaling. J Biol Chem 286, 32931-6
21795688   Curated Info

2

Kao YJ, Ghosh M, Schonbrunn A (2011) Ligand-Dependent Mechanisms of sst2A Receptor Trafficking: Role of Site-Specific Phosphorylation and Receptor Activation in the Actions of Biased Somatostatin Agonists. Mol Endocrinol 25, 1040-54
21493671   Curated Info

3

Nagel F, et al. (2011) Structural Determinants of Agonist-Selective Signaling at the sst2A Somatostatin Receptor. Mol Endocrinol 25, 859-66
21330405   Curated Info

4

Pöll F, et al. (2010) Pasireotide and octreotide stimulate distinct patterns of sst2A somatostatin receptor phosphorylation. Mol Endocrinol 24, 436-46
20051480   Curated Info

5

Liu Q, Bee MS, Schonbrunn A (2009) Site specificity of agonist and second messenger-activated kinases for somatostatin receptor subtype 2A (Sst2A) phosphorylation. Mol Pharmacol 76, 68-80
19389921   Curated Info