Thr125
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Home > Phosphorylation Site Page: > Thr125  -  CASP9 (human)

Site Information
PEVLRPEtPRPVDIG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449563
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 13 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 12 ) , mutation of modification site ( 13 , 15 , 16 ) , phospho-antibody ( 1 , 11 , 13 , 14 , 15 , 16 ) , western blotting ( 1 , 11 , 13 , 14 , 16 )
Disease tissue studied:
bone cancer ( 11 ) , breast cancer ( 3 ) , breast ductal carcinoma ( 3 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , lung cancer ( 4 , 5 , 6 ) , non-small cell lung cancer ( 5 , 6 ) , non-small cell lung adenocarcinoma ( 4 , 5 , 6 ) , non-small cell large cell lung carcinoma ( 5 ) , non-small cell squamous cell lung carcinoma ( 4 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 1 ) , 293 (epithelial) ( 11 , 16 ) , breast ( 2 , 3 ) , DMS153 (pulmonary) ( 6 ) , HCC15 (pulmonary) ( 4 ) , HCC78 (pulmonary) ( 5 ) , HeLa (cervical) ( 10 , 13 , 15 , 16 ) , Jurkat (T lymphocyte) ( 7 , 8 , 9 , 12 ) , LOU-NH91 (squamous) ( 4 ) , lung ( 4 , 5 , 6 ) , NCI-H1437 (pulmonary) ( 6 ) , NCI-H1703 (squamous) ( 4 ) , NCI-H1781 (pulmonary) ( 5 ) , NCI-H2073 (pulmonary) ( 4 , 6 ) , NCI-H209 (pulmonary) ( 6 ) , NCI-H2228 (pulmonary) ( 5 ) , NCI-H2342 (pulmonary) ( 4 ) , NCI-H3122 (pulmonary) ( 5 ) , NCI-H661 (pulmonary) ( 5 ) , NCI-H838 (pulmonary) ( 6 ) , U2OS (bone cell) ( 11 , 13 )

Upstream Regulation
Putative in vivo kinases:
CDK1 (human) ( 13 ) , ERK1 (human) ( 11 , 16 ) , ERK2 (human) ( 11 , 16 )
Kinases, in vitro:
CDK1 (human) ( 13 ) , ERK2 (human) ( 16 ) , ERK2 (rat) ( 11 )
Putative upstream phosphatases:
PPP1CA (mouse) ( 14 )
Phosphatases, in vitro:
PPP1CA (mouse) ( 14 )
Treatments:
AG1296 ( 1 ) , anisomycin ( 11 ) , EGF ( 11 , 16 ) , IL-13 ( 1 ) , IL-2_withdrawal ( 14 ) , IL-33 ( 1 ) , ischemia ( 3 ) , nocodazole ( 13 ) , okadaic_acid ( 14 , 15 ) , PD184352 ( 11 , 13 , 16 ) , PDGF ( 1 ) , phorbol_ester ( 11 , 13 , 16 ) , purvalanol ( 13 ) , SB203580 ( 11 ) , seliciclib ( 13 ) , U0126 ( 11 , 13 , 16 )

Downstream Regulation
Effects of modification on CASP9:
activity, inhibited ( 15 )
Effects of modification on biological processes:
apoptosis, altered ( 15 ) , apoptosis, induced ( 13 )

References 

1

Liao G, Wang R, Tang DD (2021) Plk1 Regulates Caspase-9 Phosphorylation at Ser-196 and Apoptosis of Human Airway Smooth Muscle Cells. Am J Respir Cell Mol Biol
34705620   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Rikova K, Hall B (2013) CST Curation Set: 20739, 21166, 30167, 30168, 30169; Year: 2013; Biosample/Treatment: cell line, H2342, H2073, Lou-NH91, HCC15, H1703; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

5

Rikova K, Hall B (2013) CST Curation Set: 20740, 21167, 30170, 30171, 30172; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

6

Rikova K, Hall B (2013) CST Curation Set: 20742, 21169, 30176, 30177, 30178; Year: 2013; Biosample/Treatment: cell line, H838, DMS153, H2073, H209, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

7

Guo A (2011) CST Curation Set: 12058; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pTXR
Curated Info

8

Guo A (2011) CST Curation Set: 12062; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pTXR
Curated Info

9

Possemato A (2010) CST Curation Set: 10290; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

10

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

11

Martin MC, Allan LA, Mancini EJ, Clarke PR (2008) The docking interaction of caspase-9 with ERK2 provides a mechanism for the selective inhibitory phosphorylation of caspase-9 at threonine 125. J Biol Chem 283, 3854-65
18083711   Curated Info

12

Zhou J (2008) CST Curation Set: 3677; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pTXR Antibodies Used to Purify Peptides prior to LCMS: Phospho-Threonine-X-Arginine Antibody Cat#: 2351, PTMScan(R) Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads Cat#: 1988
Curated Info

13

Allan LA, Clarke PR (2007) Phosphorylation of caspase-9 by CDK1/cyclin B1 protects mitotic cells against apoptosis. Mol Cell 26, 301-10
17466630   Curated Info

14

Dessauge F, et al. (2006) Identification of PP1alpha as a caspase-9 regulator in IL-2 deprivation-induced apoptosis. J Immunol 177, 2441-51
16888006   Curated Info

15

Brady SC, Allan LA, Clarke PR (2005) Regulation of caspase 9 through phosphorylation by protein kinase C zeta in response to hyperosmotic stress. Mol Cell Biol 25, 10543-55
16287866   Curated Info

16

Allan LA, et al. (2003) Inhibition of caspase-9 through phosphorylation at Thr 125 by ERK MAPK. Nat Cell Biol 5, 647-54
12792650   Curated Info

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