Ser48

Site Information
PQPsRRGsGSsEDMy SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 449320

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 ) , phospho-antibody ( 4 , 5 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:	'fat, brown' ( 3 ) , 'muscle, skeletal' ( 4 , 5 ) , heart ( 2 , 3 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 1 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 1 ) , HL-1 (myocyte) ( 1 ) , lung ( 3 )

Upstream Regulation
Treatments:	insulin ( 4 , 5 ) , racepinefrine ( 4 , 5 )

References
1	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
2	Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11 23737553 Curated Info
3	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
4	Toole BJ, Cohen PT (2007) The skeletal muscle-specific glycogen-targeted protein phosphatase 1 plays a major role in the regulation of glycogen metabolism by adrenaline in vivo. Cell Signal 19, 1044-55 17257813 Curated Info
5	Walker KS, Watt PW, Cohen P (2000) Phosphorylation of the skeletal muscle glycogen-targetting subunit of protein phosphatase 1 in response to adrenaline in vivo. FEBS Lett 466, 121-4 10648825 Curated Info