Ser330
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Home > Phosphorylation Site Page: > Ser330  -  PAI-RBP1 (human)

Site Information
FVLHksKsEEAHAED   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 476403

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 )
Disease tissue studied:
breast cancer ( 4 , 7 , 14 , 15 ) , breast ductal carcinoma ( 7 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 , 7 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 13 ) , acute myeloblastic leukemia, without maturation (M1) ( 13 ) , lung cancer ( 5 , 15 , 18 , 25 ) , non-small cell lung cancer ( 15 , 25 ) , non-small cell lung adenocarcinoma ( 5 ) , B cell lymphoma ( 13 ) , non-Hodgkin's lymphoma ( 13 ) , neuroblastoma ( 12 ) , ovarian cancer ( 7 ) , multiple myeloma ( 13 ) , prostate cancer ( 22 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 17 ) , 293 (epithelial) ( 23 ) , 293 (epithelial) [ADRB1 (human), no information, overexpresses human beta1-adrenergic (ß1AR- HEK293)] ( 27 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 21 ) , 293 (epithelial) [AT1 (human), transfection] ( 20 ) , 293T (epithelial) ( 29 ) , breast ( 2 , 7 ) , BT-474 (breast cell) ( 4 ) , BT-549 (breast cell) ( 15 ) , Calu 6 (pulmonary) ( 15 ) , CL1-0 (pulmonary) ( 18 ) , CL1-1 (pulmonary) ( 18 ) , CL1-2 (pulmonary) ( 18 ) , CL1-5 (pulmonary) ( 18 ) , CTS (myeloid) ( 13 ) , DG75 (B lymphocyte) ( 19 ) , DOHH2 ('B lymphocyte, precursor') ( 13 ) , Flp-In T-Rex-293 (epithelial) ( 16 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 16 ) , H2009 (pulmonary) ( 15 ) , H2077 (pulmonary) ( 15 ) , H2887 (pulmonary) ( 15 ) , H322 (pulmonary) ( 15 ) , H322M (pulmonary) ( 15 ) , HCC1359 (pulmonary) ( 15 ) , HCC1937 (breast cell) ( 15 ) , HCC2279 (pulmonary) ( 15 ) , HCC366 (pulmonary) ( 15 ) , HCC4006 (pulmonary) ( 15 ) , HCC78 (pulmonary) ( 15 ) , HCC827 (pulmonary) ( 15 ) , HeLa (cervical) ( 1 , 6 , 24 , 26 , 28 ) , HOP62 (pulmonary) ( 15 ) , Jurkat (T lymphocyte) ( 9 ) , K562 (erythroid) ( 11 ) , LCLC-103H (pulmonary) ( 15 ) , liver ( 8 ) , LNCaP (prostate cell) ( 22 ) , LOU-NH91 (squamous) ( 15 ) , MCF-7 (breast cell) ( 15 ) , MDA-MB-231 (breast cell) ( 15 ) , MV4-11 (macrophage) ( 13 ) , NB10 (neural crest) ( 12 ) , NCI-H1299 (pulmonary) ( 25 ) , NCI-H157 (pulmonary) ( 15 ) , NCI-H1648 (pulmonary) ( 15 ) , NCI-H1666 (pulmonary) ( 15 ) , NCI-H2172 (pulmonary) ( 15 ) , NCI-H460 (pulmonary) ( 15 ) , NCI-H520 (squamous) ( 15 ) , NPC (neural crest) ( 12 ) , OPM-2 (plasma cell) ( 13 ) , ovary ( 7 ) , P31/FUJ (erythroid) ( 13 ) , PC9 (pulmonary) ( 5 , 15 ) , RL ('B lymphocyte, precursor') ( 13 ) , RPMI-8226 (plasma cell) ( 13 ) , SKBr3 (breast cell) ( 14 ) , SU-DHL-6 (B lymphocyte) ( 13 ) , U266 (plasma cell) ( 13 )

Upstream Regulation
Regulatory protein:
PRKD1 (human) ( 16 )
Treatments:
angiotensin_2 ( 21 ) , EGF ( 1 ) , ischemia ( 7 ) , isoproterenol ( 27 ) , metastatic potential ( 18 ) , SII_angiotensin_2 ( 20 , 21 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

4

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

5

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

6

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

7

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

8

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

9

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

10

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

11

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

12

DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513
24349301   Curated Info

13

Casado P, et al. (2013) Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol 14, R37
23628362   Curated Info

14

Imami K, et al. (2012) Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways. Mol Cell Proteomics 11, 1741-57
22964224   Curated Info

15

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

16

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

17

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

18

Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97
20815410   Curated Info

19

Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72
20562096   Curated Info

20

Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304
20686112   Curated Info

21

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

22

Chen L, Giorgianni F, Beranova-Giorgianni S (2010) Characterization of the phosphoproteome in LNCaP prostate cancer cells by in-gel isoelectric focusing and tandem mass spectrometry. J Proteome Res 9, 174-8
20044836   Curated Info

23

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

24

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
19276368   Curated Info

25

Tsai CF, et al. (2008) Immobilized metal affinity chromatography revisited: pH/acid control toward high selectivity in phosphoproteomics. J Proteome Res 7, 4058-69
18707149   Curated Info

26

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

27

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

28

Yu LR, et al. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra. J Proteome Res 6, 4150-62
17924679   Curated Info

29

Molina H, et al. (2007) Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry. Proc Natl Acad Sci U S A 104, 2199-204
17287340   Curated Info