Ser41
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser41  -  GAP43 (rat)

Site Information
AATkIQAsFRGHITR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448719

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 7 , 9 ) , [32P] ATP in vitro ( 9 ) , [32P] bio-synthetic labeling ( 4 , 5 , 7 , 8 ) , immunoprecipitation ( 9 ) , mass spectrometry ( 5 ) , mutation of modification site ( 1 ) , phospho-antibody ( 2 , 3 , 7 , 9 ) , phosphoamino acid analysis ( 5 , 8 , 9 ) , phosphopeptide mapping ( 4 , 8 , 9 ) , western blotting ( 1 , 2 , 7 , 9 )
Disease tissue studied:
adrenal cancer ( 2 ) , pheochromocytoma ( 2 ) , neuroblastoma ( 1 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 3 ) , 'neuron, cortical'-brain ( 5 ) , 'neuron, superior cervical ganglion'-brain ( 8 ) , neuron-'brain, hippocampus' ( 4 , 9 ) , PC-12 (chromaffin) ( 2 ) , SH-SY5Y (neural crest) ( 1 )

Upstream Regulation
Regulatory protein:
PKCE (rat) ( 2 )
Putative in vivo kinases:
PKCA (rat) ( 5 )
Kinases, in vitro:
PKCA (human) ( 6 , 10 ) , PKCA (rat) ( 5 )
Treatments:
bisindolylmaleimide ( 2 ) , cobalt ( 2 ) , ethanol ( 3 ) , FGF2 ( 4 ) , ganglioside_GD1a ( 7 ) , ganglioside_GM1 ( 7 ) , NAC ( 2 ) , NGF ( 9 ) , okadaic_acid ( 5 ) , PD98059 ( 2 ) , phorbol_ester ( 4 , 5 , 7 , 8 , 9 ) , PKC-epsilon_translocation_inhibitor ( 2 ) , siRNA ( 2 ) , Zn(2+) ( 2 )

Downstream Regulation
Effects of modification on biological processes:
cell adhesion, altered ( 1 ) , cell growth, altered ( 1 ) , cytoskeletal reorganization ( 1 )

References 

1

Nguyen L, He Q, Meiri KF (2009) Regulation of GAP-43 at serine 41 acts as a switch to modulate both intrinsic and extrinsic behaviors of growing neurons, via altered membrane distribution. Mol Cell Neurosci 41, 62-73
19249369   Curated Info

2

Gopalakrishna R, Gundimeda U, Schiffman JE, McNeill TH (2008) A direct redox regulation of protein kinase C isoenzymes mediates oxidant-induced neuritogenesis in PC12 cells. J Biol Chem 283, 14430-44
18375950   Curated Info

3

Kim HJ, et al. (2006) Acute ethanol administration decreases GAP-43 and phosphorylated-GAP-43 in the rat hippocampus. Brain Res 1112, 16-25
16904654   Curated Info

4

Tejero-Díez P, Rodríguez-Sánchez P, Martín-Cófreces NB, Díez-Guerra FJ (2000) bFGF stimulates GAP-43 phosphorylation at ser41 and modifies its intracellular localization in cultured hippocampal neurons. Mol Cell Neurosci 16, 766-80
11124896   Curated Info

5

Edgar MA, et al. (1997) Phosphorylation of the casein kinase II domain of B-50 (GAP-43) in rat cortical growth cones. J Neurochem 69, 2206-15
9349568   Curated Info

6

He Q, Dent EW, Meiri KF (1997) Modulation of actin filament behavior by GAP-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site. J Neurosci 17, 3515-24
9133376   Curated Info

7

Meiri KF, Beverly M (1994) Effects of gangliosides GM1 and GD1a on GAP-43 phosphorylation and dephosphorylation in isolated growth cones. J Neurochem 62, 291-9
8263528   Curated Info

8

Spencer SA, Schuh SM, Liu WS, Willard MB (1992) GAP-43, a protein associated with axon growth, is phosphorylated at three sites in cultured neurons and rat brain. J Biol Chem 267, 9059-64
1533624   Curated Info

9

Meiri KF, Burdick D (1991) Nerve growth factor stimulation of GAP-43 phosphorylation in intact isolated growth cones. J Neurosci 11, 3155-64
1834809   Curated Info

10

Nielander HB, et al. (1990) Mutation of serine 41 in the neuron-specific protein B-50 (GAP-43) prohibits phosphorylation by protein kinase C. J Neurochem 55, 1442-5
2144585   Curated Info